RegulonDB

Gene
Name:	recT
Synonym(s):	ECK1346, EG11899, b1349
Genome position(nucleotides):	1413984 <-- 1414793 Genome Browser
Strand:	reverse
Sequence:	Get nucleotide sequence FastaFormat
GC content %:	47.16
External database links:

ASAP:	ABE-0004529
CGSC:	32070
ECHOBASE:	EB1844
OU-MICROARRAY:	b1349
PortEco:	recT
STRING:	511145.b1349
COLOMBOS:	recT

Product

Name:

Rac prophage; recombinase RecT

Synonym(s):

RecT, recombinase, DNA renaturation

Sequence:

Get amino acid sequence Fasta Format

Cellular location:

cytosol

Molecular weight:

29.721

Isoelectric point:

8.668

Motif(s):

Type	Positions	Sequence
	54 -> 244	RMIRIATTEIRKVPALGNCDTMSFVSAIVQCSQLGLEPGSALGHAYLLPFGNKNEKSGKKNVQLIIGYRGMIDLARRSGQIASLSARVVREGDEFSFEFGLDEKLIHRPGENEDAPVTHVYAVARLKDGGTQFEVMTRKQIELVRSLSKAGNNGPWVTHWEEMAKKTAIRRLFKYLPVSIEIQRAVSMDEK

Classification:

Multifun Terms (GenProtEC)
Multifun Terms (GenProtEC)		8 - extrachromosomal --> 8.1 - prophage genes and phage related functions
Gene Ontology Terms (GO)
	cellular_component	GO:0005829 - cytosol GO:0032993 - protein-DNA complex
	molecular_function	GO:0003677 - DNA binding GO:0003697 - single-stranded DNA binding GO:0005515 - protein binding
	biological_process	GO:0006259 - DNA metabolic process GO:0006310 - DNA recombination GO:0032508 - DNA duplex unwinding

Note(s):

Note(s): ...[more].

Evidence:

[APPH] Assay of protein purified to homogeneity
[IGI] Inferred from genetic interaction

Reference(s):

[1] Clark AJ., et al., 1993
[2] Hall SD., et al., 1993
[3] Hall SD., et al., 1994
[4] Handa N., et al., 2003
[5] Handa N., et al., 2005
[6] Kingston AW., et al., 2017
[7] Kusano K., et al., 1994
[8] Shiraishi K., et al., 2002

External database links:

DIP:

ECOCYC:

ECOLIWIKI:

INTERPRO:

INTERPRO:

PFAM:

PRIDE:

PRODB:

REFSEQ:

UNIPROT:

Operon

Name:

recET-ralR-rcbA-xisR-intR

Operon arrangement:

Transcription unit	Promoter

Elements in the selected gene context region unrelated to any object in RegulonDB

	Type	Name	Post Left	Post Right	Strand	Notes	Evidence (Confirmed, Strong, Weak)	References

Reference(s)
[1] Clark AJ., Sharma V., Brenowitz S., Chu CC., Sandler S., Satin L., Templin A., Berger I., Cohen A., 1993, Genetic and molecular analyses of the C-terminal region of the recE gene from the Rac prophage of Escherichia coli K-12 reveal the recT gene., J Bacteriol 175(23):7673-82
[2] Hall SD., Kane MF., Kolodner RD., 1993, Identification and characterization of the Escherichia coli RecT protein, a protein encoded by the recE region that promotes renaturation of homologous single-stranded DNA., J Bacteriol 175(1):277-87
[3] Hall SD., Kolodner RD., 1994, Homologous pairing and strand exchange promoted by the Escherichia coli RecT protein., Proc Natl Acad Sci U S A 91(8):3205-9
[4] Handa N., Kobayashi I., 2003, Accumulation of large non-circular forms of the chromosome in recombination-defective mutants of Escherichia coli., BMC Mol Biol 4:5
[5] Handa N., Kobayashi I., 2005, Type III restriction is alleviated by bacteriophage (RecE) homologous recombination function but enhanced by bacterial (RecBCD) function., J Bacteriol 187(21):7362-73
[6] Kingston AW., Ponkratz C., Raleigh EA., 2017, Rpn (YhgA-Like) Proteins of Escherichia coli K-12 and Their Contribution to RecA-Independent Horizontal Transfer., J Bacteriol 199(7)
[7] Kusano K., Takahashi NK., Yoshikura H., Kobayashi I., 1994, Involvement of RecE exonuclease and RecT annealing protein in DNA double-strand break repair by homologous recombination., Gene 138(1-2):17-25
[8] Shiraishi K., Hanada K., Iwakura Y., Ikeda H., 2002, Roles of RecJ, RecO, and RecR in RecET-mediated illegitimate recombination in Escherichia coli., J Bacteriol 184(17):4715-21