RegulonDB RegulonDB 11.1: Gene Form
   

rfbX gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

rfbX glf rfbA rfbC rfbXp7 rfbXp7 rfbXp8 rfbXp8 rfbXp5 rfbXp5 rfbXp6 rfbXp6 TSS_2388 TSS_2388 rfcp11 rfcp11 rfcp7 rfcp7 rfcp10 rfcp10 rfcp8 rfcp8

Gene      
Name: rfbX    Texpresso search in the literature
Synonym(s): ECK2031, EG11980, b2037, wzx, wzxB
Genome position(nucleotides): 2108337 <-- 2109584
Strand: reverse
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
 35.42
External database links:  
ASAP:
 ABE-0006757
CGSC:
 37353
ECHOBASE:
 EB1923
ECOLIHUB:
 rfbX
OU-MICROARRAY:
 b2037
STRING:
 511145.b2037
COLOMBOS: rfbX


Product      
Name: polyisoprenol-linked O16-antigen repeat unit flippase
Synonym(s): RfbX, Wzx, WzxB
Sequence: Get amino acid sequence Fasta Format
Cellular location: inner membrane
Molecular weight: 45.383
Isoelectric point: 10.292
Motif(s):
 
Type Positions Sequence Comment
9 -> 278 RRNVIYLAVVQGSNYLLPLLTFPYLVRTLGPENFGIFGFCQATMLYMIMFVEYGFNLTATQSIAKAADSKDKVTSIFWAVIFSKIVLIVITLIFLTSMTLLVPEYNKHAVIIWSFVPALVGNLIYPIWLFQGKEKMKWLTLSSILSRLAIIPLTFIFVNTKSDIAIAGFIQSSANLVAGIIALAIVVHEGWIGKVTLSLHNVRRSLADGFHVFISTSAISLYSTGIVIILGFISGPTSVGNFNAANTIRNALQGLLNPITQAIYPRISST
12 -> 32 VIYLAVVQGSNYLLPLLTFPY UniProt: Helical.
42 -> 62 FGIFGFCQATMLYMIMFVEYG UniProt: Helical.
84 -> 104 IFWAVIFSKIVLIVITLIFLT UniProt: Helical.
118 -> 138 VIIWSFVPALVGNLIYPIWLF UniProt: Helical.

 
Classification:
Multifun Terms (GenProtEC)  
  1 - metabolism --> 1.6 - biosynthesis of macromolecules (cellular constituents) --> 1.6.3 - lipopolysaccharide --> 1.6.3.1 - O antigen
  4 - transport --> 4.9.A - Transporters of Unknown Classification
  6 - cell structure --> 6.1 - membrane
Gene Ontology Terms (GO)  
cellular_component GO:0016020 - membrane
GO:0005886 - plasma membrane
GO:0005887 - integral component of plasma membrane
GO:0016021 - integral component of membrane
biological_process GO:0006974 - cellular response to DNA damage stimulus
GO:0009103 - lipopolysaccharide biosynthetic process
GO:0009243 - O antigen biosynthetic process
GO:0046677 - response to antibiotic
Note(s): Note(s): ...[more].
External database links:  
ALPHAFOLD:
 P37746
ECOCYC:
 RFBX-MONOMER
ECOLIWIKI:
 b2037
INTERPRO:
 IPR002797
PFAM:
 PF01943
PRIDE:
 P37746
REFSEQ:
 NP_416541
UNIPROT:
 P37746


Operon      
Name: rfbBDACX         
Operon arrangement:
Transcription unit        Promoter
rfbACX
rfbBDACX


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter rfcp8 2107234 reverse nd [COMP-AINF] [1]
  promoter rfcp10 2107283 reverse nd [COMP-AINF] [1]
  promoter rfcp7 2107394 reverse nd [COMP-AINF] [1]
  promoter rfcp11 2107444 reverse nd [COMP-AINF] [1]
  promoter TSS_2388 2108494 reverse nd [RS-EPT-CBR] [2]
  promoter rfbXp6 2109586 reverse nd [COMP-AINF] [1]
  promoter rfbXp5 2109676 reverse nd [COMP-AINF] [1]
  promoter rfbXp8 2109716 reverse nd [COMP-AINF] [1]
  promoter rfbXp7 2109718 reverse nd [COMP-AINF] [1]


Evidence    

 [COMP-AINF] Inferred computationally without human oversight

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates


Reference(s)    

 [1] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78

 [2] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

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