RegulonDB RegulonDB 11.1: Gene Form
   

wbbH gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

rfbX wbbH glf wbbI wbbJ rfbC TSS_2388 TSS_2388 rfcp11 rfcp11 rfcp7 rfcp7 rfcp10 rfcp10 rfcp8 rfcp8 TSS_2387 TSS_2387 TSS_2386 TSS_2386 TSS_2385 TSS_2385

Gene      
Name: wbbH    Texpresso search in the literature
Synonym(s): ECK2029, EG11982, b2035, rfc, yefF
Genome position(nucleotides): 2106060 <-- 2107226
Strand: reverse
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
 32.31
External database links:  
ASAP:
 ABE-0006753
ECHOBASE:
 EB1925
ECOLIHUB:
 rfc
OU-MICROARRAY:
 b2035
STRING:
 511145.b2035
COLOMBOS: wbbH


Product      
Name: putative O-antigen polymerase
Synonym(s): Rfc, WbbH, YefF
Sequence: Get amino acid sequence Fasta Format
Cellular location: inner membrane
Molecular weight: 44.744
Isoelectric point: 9.092
Motif(s):
 
Type Positions Sequence Comment
23 -> 43 IFYPAVCVNIIFALVLLGYEI UniProt: Helical.
57 -> 77 LIFLLCNVLTFTLSCLLTESV UniProt: Helical.
97 -> 117 VHNVGLLVISFSMIYICMRLS UniProt: Helical.
143 -> 163 NFSAYMQPIILTTFALFIWSK UniProt: Helical.
180 -> 200 IVFIFAIILNTGKQIVFMVII UniProt: Helical.

 
Classification:
Multifun Terms (GenProtEC)  
  1 - metabolism --> 1.6 - biosynthesis of macromolecules (cellular constituents) --> 1.6.3 - lipopolysaccharide --> 1.6.3.1 - O antigen
  6 - cell structure --> 6.3 - surface antigens (ECA, O antigen of LPS)
Gene Ontology Terms (GO)  
cellular_component GO:0016020 - membrane
GO:0005886 - plasma membrane
GO:0016021 - integral component of membrane
biological_process GO:0009103 - lipopolysaccharide biosynthetic process
Note(s): Note(s): ...[more].
Evidence: [COMP-HINF-FN-FROM-SEQ] Human inference of function from sequence
Reference(s): [1] Stevenson G., et al., 1994
External database links:  
ALPHAFOLD:
 P37748
ECOCYC:
 EG11982-MONOMER
ECOLIWIKI:
 b2035
PRIDE:
 P37748
PRODB:
 PRO_000023731
REFSEQ:
 NP_416539
UNIPROT:
 P37748


Operon      
Name: glf-wbbH         
Operon arrangement:
Transcription unit        Promoter
glf-rfc


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter TSS_2385 2105457 reverse nd [RS-EPT-CBR] [2]
  promoter TSS_2386 2106618 reverse nd [RS-EPT-CBR] [2]
  promoter TSS_2387 2106661 reverse nd [RS-EPT-CBR] [2]
  promoter rfcp8 2107234 reverse nd [COMP-AINF] [3]
  promoter rfcp10 2107283 reverse nd [COMP-AINF] [3]
  promoter rfcp7 2107394 reverse nd [COMP-AINF] [3]
  promoter rfcp11 2107444 reverse nd [COMP-AINF] [3]
  promoter TSS_2388 2108494 reverse nd [RS-EPT-CBR] [2]


Evidence    

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates

 [COMP-AINF] Inferred computationally without human oversight


Reference(s)    

 [1] Stevenson G., Neal B., Liu D., Hobbs M., Packer NH., Batley M., Redmond JW., Lindquist L., Reeves P., 1994, Structure of the O antigen of Escherichia coli K-12 and the sequence of its rfb gene cluster., J Bacteriol 176(13):4144-56

 [2] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

 [3] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78

RegulonDB