RegulonDB

Gene
Name:	mlrA
Synonym(s):	ECK2119, EG12008, b2127, yehV
Genome position(nucleotides):	2214866 --> 2215597 Genome Browser
Strand:	forward
Sequence:	Get nucleotide sequence FastaFormat
GC content %:	52.87
Reference(s):	[1] Brown PK., et al., 2001
External database links:

ASAP:	ABE-0007030
ECHOBASE:	EB1946
ECOLIHUB:	mlrA
OU-MICROARRAY:	b2127
STRING:	511145.b2127
COLOMBOS:	mlrA

Product

Name:

DNA-binding transcriptional activator MlrA

Synonym(s):

MlrA, YehV

Sequence:

Get amino acid sequence Fasta Format

Regulator Family:

MerR

Cellular location:

cytosol

Molecular weight:

28.046

Isoelectric point:

8.931

Motif(s):

Type	Positions	Sequence
	4 -> 72	YTIGEVALLCDINPVTLRAWQRRYGLLKPQRTDGGHRLFNDADIDRIREIKRWIDNGVQVSKVKMLLSN
	3 -> 72	LYTIGEVALLCDINPVTLRAWQRRYGLLKPQRTDGGHRLFNDADIDRIREIKRWIDNGVQVSKVKMLLSN
	6 -> 25	IGEVALLCDINPVTLRAWQR

Classification:

Multifun Terms (GenProtEC)
		2 - information transfer --> 2.2 - RNA related --> 2.2.2 - Transcription related
		3 - regulation --> 3.1 - type of regulation --> 3.1.2 - transcriptional level
Gene Ontology Terms (GO)
	cellular_component	GO:0005829 - cytosol
	molecular_function	GO:0003677 - DNA binding GO:0005515 - protein binding GO:0003700 - DNA-binding transcription factor activity
	biological_process	GO:0006355 - regulation of transcription, DNA-templated

Note(s):

Note(s): ...[more].

Evidence:

[AIFS] Automated inference of function from sequence
[APPHINH] Assay of protein purified to homogeneity from its native host
[IEP] Inferred from expression pattern
[IHBCE] Inferred by a human based on computational evidence

Reference(s):

[1] Brown PK., et al., 2001
[2] Ogasawara H., et al., 2010
[3] Perez-Rueda E., et al., 2004
[4] Shaikh N., et al., 2003

External database links:

ECOCYC:

ECOLIWIKI:

INTERPRO:

INTERPRO:

MINT:

MODBASE:

PFAM:

PRIDE:

PRODB:

PROSITE:

PROSITE:

REFSEQ:

SMART:

SMR:

UNIPROT:

Operon

Name:

mlrA

Operon arrangement:

Transcription unit	Promoter
mlrA	mlrAp
mlrA	mlrAp2

Transcriptional Regulation
	Display Regulation
Repressed by:	FliZ

Elements in the selected gene context region unrelated to any object in RegulonDB

Type	Name	Post Left	Strand	Notes	Evidence (Confirmed, Strong, Weak)	References
promoter	TSS_2442	2212969	reverse	nd	[RS-EPT-CBR]	[5]
promoter	TSS_2443	2214673	reverse	nd	[RS-EPT-CBR]	[5]
promoter	mlrAp6	2214749	forward	Similarity to the consensus sequence of the set of known functional promoters for this sigma factor with strong experimental evidence: good homology. Score: 3.74. P-value: 7.791e-5 Read more >	[ICWHO]	[6]
promoter	mlrAp9	2214785	forward	Similarity to the consensus sequence of the set of known functional promoters for this sigma factor with strong experimental evidence: good homology. Score: 4.615. P-value: 2.123e-5 Read more >	[ICWHO]	[6]
promoter	yohOp6	2215586	forward	Similarity to the consensus sequence of the set of known functional promoters for this sigma factor with strong experimental evidence: high homology. Score: 4.28. P-value: 4.276e-5 Read more >	[ICWHO]	[6]

Evidence
[RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates
[ICWHO] Inferred computationally without human oversight

Reference(s)
[1] Brown PK., Dozois CM., Nickerson CA., Zuppardo A., Terlonge J., Curtiss R., 2001, MlrA, a novel regulator of curli (AgF) and extracellular matrix synthesis by Escherichia coli and Salmonella enterica serovar Typhimurium., Mol Microbiol 41(2):349-63
[2] Ogasawara H., Yamamoto K., Ishihama A., 2010, Regulatory role of MlrA in transcription activation of csgD, the master regulator of biofilm formation in Escherichia coli., FEMS Microbiol Lett 312(2):160-8
[3] Perez-Rueda E., Collado-Vides J., Segovia L., 2004, Phylogenetic distribution of DNA-binding transcription factors in bacteria and archaea., Comput Biol Chem 28(5-6):341-50
[4] Shaikh N., Tarr PI., 2003, Escherichia coli O157:H7 Shiga toxin-encoding bacteriophages: integrations, excisions, truncations, and evolutionary implications., J Bacteriol 185(12):3596-605
[5] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.
[6] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78