RegulonDB RegulonDB 10.9: Gene Form
   

dusC gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

dusC yohJ yohP yohK terminator TSS_2454 TSS_2454 yohJp yohJp yohJp4 yohJp4 dusCp dusCp

Gene      
Name: dusC    Texpresso search in the literature
Synonym(s): ECK2133, EG12022, b2140, yohI
Genome position(nucleotides): 2229438 <-- 2230385 Genome Browser
Strand: reverse
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
 54.22
External database links:  
ASAP:
 ABE-0007074
ECHOBASE:
 EB1957
ECOLIHUB:
 dusC
OU-MICROARRAY:
 b2140
STRING:
 511145.b2140
COLOMBOS: dusC


Product      
Name: tRNA-dihydrouridine16 synthase
Synonym(s): DusC, YohI
Sequence: Get amino acid sequence Fasta Format
Cellular location: cytosol
Molecular weight: 35.199
Isoelectric point: 6.517
Motif(s):
 
Type Positions Sequence
98 -> 98 C
5 -> 306 LAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVKVFHRICPELQNASRTPSGTLVRVQLLGQFPQWLAENAARAVELGSWGVDLNCGCPSKTVNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQAGATELVVHGRTKEQGYRAEHIDWQAIGDIRQRLNIPVIANGEIWDWQSAQQCMAISGCDAVMIGRGALNIPNLSRVVKYNEPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQWLSYLRKEYDEATELFQHVRVLNNSPDIARA
224 -> 225 GR
7 -> 9 PME
200 -> 202 NGE

 
Classification:
Multifun Terms (GenProtEC)  
  2 - information transfer --> 2.2 - RNA related --> 2.2.3 - RNA modification
Gene Ontology Terms (GO)  
cellular_component GO:0005829 - cytosol
molecular_function GO:0003824 - catalytic activity
GO:0016491 - oxidoreductase activity
GO:0003723 - RNA binding
GO:0000049 - tRNA binding
GO:0050660 - flavin adenine dinucleotide binding
GO:0010181 - FMN binding
GO:0017150 - tRNA dihydrouridine synthase activity
biological_process GO:0008033 - tRNA processing
GO:0055114 - oxidation-reduction process
GO:0002943 - tRNA dihydrouridine synthesis
Note(s): Note(s): ...[more].
External database links:  
DIP:
 DIP-12808N
ECOCYC:
 EG12022-MONOMER
ECOLIWIKI:
 b2140
INTERPRO:
 IPR018517
INTERPRO:
 IPR035587
INTERPRO:
 IPR013785
INTERPRO:
 IPR042270
INTERPRO:
 IPR001269
INTERPRO:
 IPR032886
MODBASE:
 P33371
PANTHER:
 PTHR11082:SF26
PDB:
 4YCP
PDB:
 4YCO
PDB:
 4BFA
PDB:
 4BF9
PDB:
 3W9Z
PFAM:
 PF01207
PRIDE:
 P33371
PRODB:
 PRO_000022492
PROSITE:
 PS01136
REFSEQ:
 NP_416645
SMR:
 P33371
UNIPROT:
 P33371


Operon      
Name: dusC         
Operon arrangement:
Transcription unit        Promoter
dusC


RNA cis-regulatory element    
Attenuation: Translational


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter yohJp4 2230493 forward Similarity to the consensus
Read more > 		[ICWHO] [1]
  promoter TSS_2454 2230603 forward nd [RS-EPT-CBR] [2]


Evidence    

 [ICWHO] Inferred computationally without human oversight

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates


Reference(s)    

 [1] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78

 [2] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

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