RegulonDB RegulonDB 11.1: Gene Form
   

yeiG gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

cirA yeiG folE MetJ MetJ MetJ MetJ yeiGp6 yeiGp6 TSS_2458 (cluster) TSS_2458 (cluster) folEp folEp TSS_2457 TSS_2457 TSS_2456 TSS_2456 yeiBp2 yeiBp2

Gene      
Name: yeiG    Texpresso search in the literature
Synonym(s): ECK2147, EG12026, b2154
Genome position(nucleotides): 2243910 --> 2244746
Strand: forward
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
 54.48
External database links:  
ASAP:
 ABE-0007124
ECHOBASE:
 EB1961
ECOLIHUB:
 yeiG
OU-MICROARRAY:
 b2154
STRING:
 511145.b2154
COLOMBOS: yeiG


Product      
Name: S-formylglutathione hydrolase / S-lactoylglutathione hydrolase
Synonym(s): YeiG
Sequence: Get amino acid sequence Fasta Format
Cellular location: cytosol
Molecular weight: 31.259
Isoelectric point: 5.08
Motif(s):
 
Type Positions Sequence Comment
21 -> 271 SSTLNCPMTFSIFLPPPRDHTPPPVLYWLSGLTCNDENFTTKAGAQRVAAELGIVLVMPDTSPRGEKVANDDGYDLGQGAGFYLNATQPPWATHYRMYDYLRDELPALVQSQFNVSDRCAISGHSMGGHGALIMALKNPGKYTSVSAFAPIVNPCSVPWGIKAFSSYLGEDKNAWLEWDSCALMYASNAQDAIPTLIDQGDNDQFLADQLQPAVLAEAARQKAWPMTLRIQPGYDHSYYFIASFIEDHLRF
26 -> 26 C UniProt: Reduces catalytic efficiency, but has no effect on affinity for substrate..
54 -> 54 C UniProt: Reduces affinity for substrate and catalytic efficiency..
80 -> 80 D UniProt: Increases affinity for substrate, but reduces catalytic efficiency..
145 -> 145 S UniProt: Loss of activity..

 
Classification:
Multifun Terms (GenProtEC)  
  5 - cell processes --> 5.6 - protection --> 5.6.2 - detoxification
Gene Ontology Terms (GO)  
cellular_component GO:0005829 - cytosol
GO:0032991 - protein-containing complex
molecular_function GO:0016787 - hydrolase activity
GO:0018738 - S-formylglutathione hydrolase activity
GO:0004416 - hydroxyacylglutathione hydrolase activity
GO:0042802 - identical protein binding
GO:0052689 - carboxylic ester hydrolase activity
biological_process GO:0019243 - methylglyoxal catabolic process to D-lactate via S-lactoyl-glutathione
GO:0046294 - formaldehyde catabolic process
GO:0051289 - protein homotetramerization
Note(s): Note(s): ...[more].
Reference(s): [1] McCloskey D., et al., 2018
External database links:  
ALPHAFOLD:
 P33018
DIP:
 DIP-11919N
ECOCYC:
 EG12026-MONOMER
ECOLIWIKI:
 b2154
INTERPRO:
 IPR014186
INTERPRO:
 IPR000801
INTERPRO:
 IPR029058
MODBASE:
 P33018
PANTHER:
 PTHR10061
PFAM:
 PF00756
PRIDE:
 P33018
REFSEQ:
 NP_416659
SMR:
 P33018
UNIPROT:
 P33018


Operon      
Name: yeiG         
Operon arrangement:
Transcription unit        Promoter
yeiG


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter yeiBp2 2243073 reverse nd [COMP-AINF] [2]
  promoter TSS_2456 2243404 reverse nd [RS-EPT-CBR] [3]
  promoter TSS_2457 2243799 reverse nd [RS-EPT-CBR] [3]
  promoter TSS_2458 (cluster) 2243879 forward nd [RS-EPT-CBR] [3]
  promoter yeiGp6 2243888 forward nd [COMP-AINF] [2]


Evidence    

 [COMP-AINF] Inferred computationally without human oversight

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates


Reference(s)    

 [1] McCloskey D., Xu S., Sandberg TE., Brunk E., Hefner Y., Szubin R., Feist AM., Palsson BO., 2018, Adaptation to the coupling of glycolysis to toxic methylglyoxal production in tpiA deletion strains of Escherichia coli requires synchronized and counterintuitive genetic changes., Metab Eng 48:82-93

 [2] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78

 [3] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

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