RegulonDB RegulonDB 11.1: Gene Form
   

yojI gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

mqo yojI alkB Lrp Lrp Lrp Lrp Lrp Lrp terminator yojIp yojIp mqop1 mqop1 mqop5 mqop5 mqop8 mqop8

Gene      
Name: yojI    Texpresso search in the literature
Synonym(s): ECK2203, EG12070, b2211, yojJ
Genome position(nucleotides): 2306972 <-- 2308615
Strand: reverse
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
 53.41
External database links:  
ASAP:
 ABE-0007303
ECHOBASE:
 EB1997
ECOLIHUB:
 yojI
OU-MICROARRAY:
 b2211
STRING:
 511145.b2211
COLOMBOS: yojI


Product      
Name: ABC transporter family protein / microcin J25 efflux protein
Synonym(s): YojI, YojJ
Sequence: Get amino acid sequence Fasta Format
Cellular location: inner membrane
Molecular weight: 61.553
Isoelectric point: 7.19
Motif(s):
 
Type Positions Sequence Comment
15 -> 291 FISVMALSLASAALGIGLIAFINQRLIETADTSLLVLPEFLGLLLLLMAVTLGSQLALTTLGHHFVYRLRSEFIKRILDTHVERIEQLGSASLLAGLTSDVRNITIAFVRLPELVQGIILTIGSAAYLWMLSGKMLLVTAIWMAITIWGGFVLVARVYKHMATLRETEDKLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDAQEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLRTPLLSAVGALPTLLT UniProt: ABC transmembrane type-1.
16 -> 36 ISVMALSLASAALGIGLIAFI UniProt: Helical.
47 -> 67 SLLVLPEFLGLLLLLMAVTLG UniProt: Helical.
125 -> 145 LPELVQGIILTIGSAAYLWML UniProt: Helical.
149 -> 169 MLLVTAIWMAITIWGGFVLVA UniProt: Helical.

 
Classification:
Multifun Terms (GenProtEC)  
  6 - cell structure --> 6.1 - membrane
  4 - transport --> 4.3 - Primary Active Transporters --> 4.3.A - Pyrophosphate Bond (ATP; GTP; P2) Hydrolysis-driven Active Transporters --> 4.3.A.1 - The ATP-binding Cassette (ABC) Superfamily + ABC-type Uptake Permeases --> 4.3.A.1.am - ABC superfamily, ATP binding and membrane component
Gene Ontology Terms (GO)  
cellular_component GO:0016020 - membrane
GO:0005886 - plasma membrane
GO:0016021 - integral component of membrane
GO:0043190 - ATP-binding cassette (ABC) transporter complex
molecular_function GO:0140359 - ABC-type transporter activity
GO:0042626 - ATPase-coupled transmembrane transporter activity
GO:0000166 - nucleotide binding
GO:0005524 - ATP binding
GO:1904680 - peptide transmembrane transporter activity
biological_process GO:0046677 - response to antibiotic
GO:0015833 - peptide transport
GO:0042884 - microcin transport
GO:0055085 - transmembrane transport
Note(s): Note(s): ...[more].
Reference(s): [1] Feng Y., et al., 2018
[2] Jindal S., et al., 2019
[3] Upadhyay HC., et al., 2014
[4] Yamada S., et al., 2006
External database links:  
ALPHAFOLD:
 P33941
ECOCYC:
 YOJI-MONOMER
ECOLIWIKI:
 b2211
INTERPRO:
 IPR027417
INTERPRO:
 IPR036640
INTERPRO:
 IPR017871
INTERPRO:
 IPR011527
INTERPRO:
 IPR005898
INTERPRO:
 IPR003593
INTERPRO:
 IPR003439
MODBASE:
 P33941
PANTHER:
 PTHR43553:SF11
PFAM:
 PF00005
PRIDE:
 P33941
PROSITE:
 PS50893
PROSITE:
 PS00211
PROSITE:
 PS50929
REFSEQ:
 NP_416715
SMART:
 SM00382
UNIPROT:
 P33941


Operon      
Name: yojI         
Operon arrangement:
Transcription unit        Promoter
yojI


Transcriptional Regulation      
Display Regulation             
Activated by: Lrp


RNA cis-regulatory element    
Attenuation: Translational


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter mqop8 2306846 reverse nd [COMP-AINF] [5]
  promoter mqop5 2306858 reverse nd [COMP-AINF] [5]
  promoter mqop1 2306924 reverse nd [COMP-AINF] [5]


Evidence    

 [COMP-AINF] Inferred computationally without human oversight


Reference(s)    

 [1] Feng Y., Chen CS., Ho J., Pearce D., Hu S., Wang B., Desai P., Kim KS., Zhu H., 2018, High-Throughput Chip Assay for Investigating Escherichia coli Interaction with the Blood-Brain Barrier Using Microbial and Human Proteome Microarrays (Dual-Microarray Technology)., Anal Chem 90(18):10958-10966

 [2] Jindal S., Yang L., Day PJ., Kell DB., 2019, Involvement of multiple influx and efflux transporters in the accumulation of cationic fluorescent dyes by Escherichia coli., BMC Microbiol 19(1):195

 [3] Upadhyay HC., Dwivedi GR., Roy S., Sharma A., Darokar MP., Srivastava SK., 2014, Phytol derivatives as drug resistance reversal agents., ChemMedChem 9(8):1860-8

 [4] Yamada S., Awano N., Inubushi K., Maeda E., Nakamori S., Nishino K., Yamaguchi A., Takagi H., 2006, Effect of drug transporter genes on cysteine export and overproduction in Escherichia coli., Appl Environ Microbiol 72(7):4735-42

 [5] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78

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