RegulonDB

Gene
Name:	tktB
Synonym(s):	ECK2460, EG12100, b2465
Genome position(nucleotides):	2579636 --> 2581639
Strand:	forward
Sequence:	Get nucleotide sequence FastaFormat
GC content %:	54.64
External database links:

ASAP:	ABE-0008117
CGSC:	29992
ECHOBASE:	EB2024
ECOLIHUB:	tktB
OU-MICROARRAY:	b2465
STRING:	511145.b2465
COLOMBOS:	tktB

Gene

Name:

tktB

Synonym(s):

ECK2460, EG12100, b2465

Genome position(nucleotides):

2579636 --> 2581639

Strand:

forward

Sequence:

Get nucleotide sequence FastaFormat

GC content %:

54.64

External database links:

ASAP:

CGSC:

ECHOBASE:

ECOLIHUB:

OU-MICROARRAY:

STRING:

COLOMBOS:

Type

Positions

Sequence

Comment

3 -> 333

RKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFLKHNPTDPTWYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPEIGYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEGISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVIHEIDGHDPQAVKEAILEAQSVKDKPSLIICRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKLGWHHPPFEIPKEIYHAWDAREKGEKAQQSWNEKFAAYKKAHPQLAEEFTRRMSGGLP

113 -> 115

GPL

UniProt: Thiamine pyrophosphate.

353 -> 523

KIATRKASQNTLNAYGPMLPELLGGSADLAPSNLTIWKGSVSLKEDPAGNYIHYGVREFGMTAIANGIAHHGGFVPYTATFLMFVEYARNAARMAALMKARQIMVYTHDSIGLGEDGPTHQAVEQLASLRLTPNFSTWRPCDQVEAAVGWKLAVERHNGPTALILSRQNLA

539 -> 654

GYVLKDSGGKPDIILIATGSEMEITLQAAEKLAGEGRNVRVVSLPSTDIFDAQDEEYRESVLPSNVAARVAVEAGIADYWYKYVGLKGAIVGMTGYGESAPADKLFPFFGFTAENI

Type

Positions

Sequence

Comment

3 -> 333

113 -> 115

GPL

UniProt: Thiamine pyrophosphate.

353 -> 523

KIATRKASQNTLNAYGPMLPELLGGSADLAPSNLTIWKGSVSLKEDPAGNYIHYGVREFGMTAIANGIAHHGGFVPYTATFLMFVEYARNAARMAALMKARQIMVYTHDSIGLGEDGPTHQAVEQLASLRLTPNFSTWRPCDQVEAAVGWKLAVERHNGPTALILSRQNLA

539 -> 654

GYVLKDSGGKPDIILIATGSEMEITLQAAEKLAGEGRNVRVVSLPSTDIFDAQDEEYRESVLPSNVAARVAVEAGIADYWYKYVGLKGAIVGMTGYGESAPADKLFPFFGFTAENI

Multifun Terms (GenProtEC)

1 - metabolism --> 1.1 - carbon utilization --> 1.1.1 - carbon compounds

1 - metabolism --> 1.7 - central intermediary metabolism --> 1.7.3 - non-oxidative branch, pentose pathway

Gene Ontology Terms (GO)

cellular_component

GO:0005829 - cytosol

molecular_function

GO:0003824 - catalytic activity
GO:0016740 - transferase activity
GO:0046872 - metal ion binding
GO:0004802 - transketolase activity

biological_process

GO:0009052 - pentose-phosphate shunt, non-oxidative branch
GO:0006098 - pentose-phosphate shunt

Transcriptional Regulation
	Display Regulation
Activated by:	PhoB

Transcriptional Regulation

Display Regulation

Activated by:

PhoB

	Type	Name	Post Left	Post Right	Strand	Notes	Evidence (Confirmed, Strong, Weak)	References
	promoter	TSS_2750	2578793		reverse	nd	[RS-EPT-CBR]	[4]

Type

Name

Post Left

Post Right

Strand

Notes

Evidence (Confirmed, Strong, Weak)

References

promoter

TSS_2750

2578793

reverse

[RS-EPT-CBR]

[4]

Evidence
[RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates

Evidence

[RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates

Reference(s)
[1] Li RD., Liu L., 2012, Characterizing criticality of proteins by systems dynamics: Escherichia coli central carbon metabolism as a working example., BMC Syst Biol 6 Suppl 1:S11
[2] Matsuoka Y., Shimizu K., 2013, Catabolite regulation analysis of Escherichia coli for acetate overflow mechanism and co-consumption of multiple sugars based on systems biology approach using computer simulation., J Biotechnol 168(2):155-73
[3] Zhang Z., Zhang Q., Guan S., Shi H., 2020, Quantitative Connection between Cell Size and Growth Rate by Phospholipid Metabolism., Cells 9(2)
[4] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

Reference(s)

[1] Li RD., Liu L., 2012, Characterizing criticality of proteins by systems dynamics: Escherichia coli central carbon metabolism as a working example., BMC Syst Biol 6 Suppl 1:S11

[2] Matsuoka Y., Shimizu K., 2013, Catabolite regulation analysis of Escherichia coli for acetate overflow mechanism and co-consumption of multiple sugars based on systems biology approach using computer simulation., J Biotechnol 168(2):155-73

[3] Zhang Z., Zhang Q., Guan S., Shi H., 2020, Quantitative Connection between Cell Size and Growth Rate by Phospholipid Metabolism., Cells 9(2)

[4] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.