RegulonDB RegulonDB 10.9: Gene Form
   

pitA gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

pitA yhiN uspB pitAp pitAp yhiNp6 yhiNp6

Gene      
Name: pitA    Texpresso search in the literature
Synonym(s): ECK3478, EG12230, b3493, pit
Genome position(nucleotides): 3637642 --> 3639141 Genome Browser
Strand: forward
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
54.07
External database links:  
ASAP:
ABE-0011407
ECHOBASE:
EB2142
ECOLIHUB:
pitA
OU-MICROARRAY:
b3493
STRING:
511145.b3493
COLOMBOS: pitA


Product      
Name: metal phosphate:H+ symporter PitA
Synonym(s): Pit, PitA
Sequence: Get amino acid sequence Fasta Format
Cellular location: inner membrane
Molecular weight: 53.389
Isoelectric point: 9.984
Motif(s):
 
Type Positions Sequence
124 -> 144 LIGAIIGIGLTNALMTGTSVV
94 -> 114 LAMVFSMLLAAIIWNLGTWYF
155 -> 175 IFGSLIVSPIVGLVFAGGLIF
30 -> 488 NGFHDTANAVATVIYTRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLLAAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLIVSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSAIGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQHPALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLTTDVESYDKLSLDQRSQMRRIMLCVSDTIDKVVKMPGVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVALALGIGTMIGWRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVLSSSVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAVLLS
207 -> 227 PFWTRIALILSAIGVAFSHGA

 

Classification:
Multifun Terms (GenProtEC)  
  1 - metabolism --> 1.8 - metabolism of other compounds --> 1.8.1 - phosphorous metabolism
  4 - transport --> 4.2 - Electrochemical potential driven transporters --> 4.2.A - Porters (Uni-, Sym- and Antiporters)
  6 - cell structure --> 6.1 - membrane
Gene Ontology Terms (GO)  
cellular_component GO:0016020 - membrane
GO:0005886 - plasma membrane
GO:0005887 - integral component of plasma membrane
GO:0016021 - integral component of membrane
molecular_function GO:0005515 - protein binding
GO:0015295 - solute:proton symporter activity
GO:0005385 - zinc ion transmembrane transporter activity
GO:0005315 - inorganic phosphate transmembrane transporter activity
GO:1901683 - arsenate ion transmembrane transporter activity
GO:0015654 - tellurite transmembrane transporter activity
biological_process GO:0010960 - magnesium ion homeostasis
GO:0006817 - phosphate ion transport
GO:0071577 - zinc ion transmembrane transport
GO:0035435 - phosphate ion transmembrane transport
GO:1901684 - arsenate ion transmembrane transport
GO:1902600 - proton transmembrane transport
GO:0015710 - tellurite transport
Note(s): Note(s): ...[more].
Reference(s): [1] Iglesias Neves H., et al., 2015
[2] Jindal S., et al., 2019
[3] Willsky GR., et al., 1976
External database links:  
ECOCYC:
PITA-MONOMER
ECOLIWIKI:
b3493
INTERPRO:
IPR001204
PANTHER:
PTHR11101
PFAM:
PF01384
PRIDE:
P0AFJ7
PRODB:
PRO_000023554
REFSEQ:
NP_417950
UNIPROT:
P0AFJ7


Operon      
Name: pitA         
Operon arrangement:
Transcription unit        Promoter
pitA


Transcriptional Regulation      
Display Regulation             
Activated by: FNR


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter yhiNp6 3637500 reverse Similarity to the consensus
Read more >
[ICWHO] [4]


Evidence    

 [ICWHO] Inferred computationally without human oversight



Reference(s)    

 [1] Iglesias Neves H., Pereira TF., Yagil E., Spira B., 2015, Ugp and PitA participate in the selection of PHO-constitutive mutants., J Bacteriol 197(8):1378-85

 [2] Jindal S., Yang L., Day PJ., Kell DB., 2019, Involvement of multiple influx and efflux transporters in the accumulation of cationic fluorescent dyes by Escherichia coli., BMC Microbiol 19(1):195

 [3] Willsky GR., Malamy MH., 1976, Control of the synthesis of alkaline phosphatase and the phosphate-binding protein in Escherichia coli., J Bacteriol 127(1):595-609

 [4] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78


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