RegulonDB RegulonDB 11.1: Gene Form
   

gadW gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

mdtF gadW gadY GadX GadX GadX GadW GadX GadW PhoP YdeO gadYp gadYp gadWp2 gadWp2 gadWp1 gadWp1

Gene      
Name: gadW    Texpresso search in the literature
Synonym(s): ECK3499, EG12242, b3515, yhiW
Genome position(nucleotides): 3663890 <-- 3664618
Strand: reverse
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
 41.84
Note(s): ArcA appears to activate gadW gene expression under anaerobiosis. A putative ArcA binding site was identified 131 bp upstream of this gene Salmon KA,2005, but no promoter upstream of it has been identified.
Reference(s): [1] Ma Z., et al., 2002
External database links:  
ASAP:
 ABE-0011482
ECHOBASE:
 EB2153
ECOLIHUB:
 gadW
OU-MICROARRAY:
 b3515
STRING:
 511145.b3515
COLOMBOS: gadW


Product      
Name: DNA-binding transcriptional dual regulator GadW
Synonym(s): GadW, YhiW
Sequence: Get amino acid sequence Fasta Format
Regulator Family: AraC/XylS
Cellular location: cytosol
Molecular weight: 28.028
Isoelectric point: 8.77
Motif(s):
 
Type Positions Sequence Comment
139 -> 236 GKVERLISFDIAKRWYLRDIAERMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYFINTFRQYYGVTPHQFAQH UniProt: HTH araC/xylS-type.
156 -> 177 RDIAERMYTSESLIKKKLQDEN UniProt: H-T-H motif.
159 -> 235 AERMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYFINTFRQYYGVTPHQFAQ
203 -> 226 LHTIAEKCGYSSTSYFINTFRQYY UniProt: H-T-H motif.

 
Classification:
Multifun Terms (GenProtEC)  
  2 - information transfer --> 2.2 - RNA related --> 2.2.2 - Transcription related
  3 - regulation --> 3.1 - type of regulation --> 3.1.2 - transcriptional level --> 3.1.2.3 - repressor
Gene Ontology Terms (GO)  
cellular_component GO:0005829 - cytosol
molecular_function GO:0003677 - DNA binding
GO:0003700 - DNA-binding transcription factor activity
GO:0043565 - sequence-specific DNA binding
biological_process GO:0006351 - transcription, DNA-templated
GO:0006355 - regulation of transcription, DNA-templated
GO:0006974 - cellular response to DNA damage stimulus
Note(s): Note(s): ...[more].
Evidence: [EXP] Inferred from experiment
[EXP-IDA-PURIFIED-PROTEIN] Assay of protein purified to homogeneity
Reference(s): [1] Ma Z., et al., 2002
External database links:  
ALPHAFOLD:
 P63201
ECOCYC:
 EG12242-MONOMER
ECOLIWIKI:
 b3515
INTERPRO:
 IPR018062
INTERPRO:
 IPR020449
INTERPRO:
 IPR018060
INTERPRO:
 IPR009057
MODBASE:
 P63201
PFAM:
 PF12833
PRIDE:
 P63201
PRINTS:
 PR00032
PRODB:
 PRO_000022744
PROSITE:
 PS01124
PROSITE:
 PS00041
REFSEQ:
 NP_417972
SMART:
 SM00342
SMR:
 P63201
UNIPROT:
 P63201


Operon      
Name: gadAXW         
Operon arrangement:
Transcription unit        Promoter
gadW
gadW
gadX
gadXW
gadAX
gadAX


Transcriptional Regulation      
Display Regulation             
Activated by: PhoP, GadX, PhoB, SdiA, YdeO, GadE
Repressed by: GadX, FNR, H-NS, GadW, RutR


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References


Reference(s)    

 [1] Ma Z., Richard H., Tucker DL., Conway T., Foster JW., 2002, Collaborative regulation of Escherichia coli glutamate-dependent acid resistance by two AraC-like regulators, GadX and GadW (YhiW)., J Bacteriol 184(24):7001-12

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