RegulonDB RegulonDB 10.9: Gene Form
   

bcsZ gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

bcsC bcsB pdeK bcsZ TSS_4136 TSS_4136 TSS_4135 TSS_4135 yhjKp2 yhjKp2

Gene      
Name: bcsZ    Texpresso search in the literature
Synonym(s): ECK3516, EG12258, b3531, bcsC, yhjM
Genome position(nucleotides): 3689155 <-- 3690261 Genome Browser
Strand: reverse
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
54.74
External database links:  
ASAP:
ABE-0011541
ECHOBASE:
EB2167
ECOLIHUB:
bcsZ
OU-MICROARRAY:
b3531
STRING:
511145.b3531
COLOMBOS: bcsZ


Product      
Name: endo-1,4-D-glucanase
Synonym(s): BcsC, BcsZ, YhjM
Sequence: Get amino acid sequence Fasta Format
Cellular location: extracellular space
Molecular weight: 41.7
Isoelectric point: 8.873
Motif(s):
 
Type Positions Sequence
1 -> 346 MNVLRSGIVTMLLLAAFSVQAACTWPAWEQFKKDYISQEGRVIDPSDARKITTSEGQSYGMFSALAANDRAAFDNILDWTQNNLAQGSLKERLPAWLWGKKENSKWEVLDSNSASDGDVWMAWSLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLGSMLLPGKVGFAEDNSWRFNPSYLPPTLAQYFTRFGAPWTTLRETNQRLLLETAPKGFSPDWVRYEKDKGWQLKAEKTLISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTEKNGYPPEKVDVATGKAQGKGPVGFSAAMLPFLQNRDAQAVQRQRVADNFPGSDAYYNYVLTLFGQGWDQH
22 -> 368 ACTWPAWEQFKKDYISQEGRVIDPSDARKITTSEGQSYGMFSALAANDRAAFDNILDWTQNNLAQGSLKERLPAWLWGKKENSKWEVLDSNSASDGDVWMAWSLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLGSMLLPGKVGFAEDNSWRFNPSYLPPTLAQYFTRFGAPWTTLRETNQRLLLETAPKGFSPDWVRYEKDKGWQLKAEKTLISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTEKNGYPPEKVDVATGKAQGKGPVGFSAAMLPFLQNRDAQAVQRQRVADNFPGSDAYYNYVLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH

 

Classification:
Multifun Terms (GenProtEC)  
  1 - metabolism --> 1.2 - degradation of macromolecules --> 1.2.4 - polysaccharides
Gene Ontology Terms (GO)  
cellular_component GO:0005576 - extracellular region
molecular_function GO:0016787 - hydrolase activity
GO:0004553 - hydrolase activity, hydrolyzing O-glycosyl compounds
GO:0016798 - hydrolase activity, acting on glycosyl bonds
GO:0008810 - cellulase activity
biological_process GO:0008152 - metabolic process
GO:0000272 - polysaccharide catabolic process
GO:0005975 - carbohydrate metabolic process
GO:0030245 - cellulose catabolic process
Note(s): Note(s): ...[more].
Reference(s): [1] Acheson JF., et al., 2019
[2] Krasteva PV., et al., 2017
[3] Omadjela O., et al., 2013
External database links:  
CAZY:
GH8
ECOCYC:
EG12258-MONOMER
ECOLIWIKI:
b3531
INTERPRO:
IPR012341
INTERPRO:
IPR019834
INTERPRO:
IPR002037
INTERPRO:
IPR008928
MODBASE:
P37651
PDB:
3QXQ
PDB:
3QXF
PFAM:
PF01270
PRIDE:
P37651
PRINTS:
PR00735
PRODB:
PRO_000022205
PROSITE:
PS00812
REFSEQ:
NP_417988
SMR:
P37651
UNIPROT:
P37651


Operon      
Name: bcsABZC         
Operon arrangement:
Transcription unit        Promoter
bcsBZ
bcsABZC


Transcriptional Regulation      
Display Regulation             
Activated by: FNR


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter yhjKp2 3685648 reverse Similarity to the consensus
Read more >
[ICWHO] [4]
  promoter TSS_4135 3686203 reverse nd [RS-EPT-CBR] [5]
  promoter TSS_4136 3687811 reverse nd [RS-EPT-CBR] [5]


Evidence    

 [ICWHO] Inferred computationally without human oversight

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates



Reference(s)    

 [1] Acheson JF., Derewenda ZS., Zimmer J., 2019, Architecture of the Cellulose Synthase Outer Membrane Channel and Its Association with the Periplasmic TPR Domain., Structure 27(12):1855-1861.e3

 [2] Krasteva PV., Bernal-Bayard J., Travier L., Martin FA., Kaminski PA., Karimova G., Fronzes R., Ghigo JM., 2017, Insights into the structure and assembly of a bacterial cellulose secretion system., Nat Commun 8(1):2065

 [3] Omadjela O., Narahari A., Strumillo J., Melida H., Mazur O., Bulone V., Zimmer J., 2013, BcsA and BcsB form the catalytically active core of bacterial cellulose synthase sufficient for in vitro cellulose synthesis., Proc Natl Acad Sci U S A 110(44):17856-61

 [4] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78

 [5] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.


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