RegulonDB

Gene
Name:	znuB
Synonym(s):	ECK1860, EG12368, b1859, yebI
Genome position(nucleotides):	1943414 --> 1944199 Genome Browser
Strand:	forward
Sequence:	Get nucleotide sequence FastaFormat
GC content %:	51.91
External database links:

ASAP:	ABE-0006201
ECHOBASE:	EB2271
OU-MICROARRAY:	b1859
PortEco:	znuB
STRING:	511145.b1859
COLOMBOS:	znuB

Product

Name:

Zn²⁺ ABC transporter membrane subunit

Synonym(s):

YebI, ZnuB

Sequence:

Get amino acid sequence Fasta Format

Cellular location:

inner membrane

Molecular weight:

27.729

Isoelectric point:

8.712

Motif(s):

Type	Positions	Sequence
	1 -> 257	MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLLGVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLAIDTLLGIMAHSALSLGLVVVSLMSNIRVDLMAYLFGDLLAVTPEDLISIAIGVVIVVAILFWQWRNLLSMTISPDLAFVDGVKLQRVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAVLVGMVAVTGGLTFSAVYDTPAGPSVVLCAALLFILSMMK
	84 -> 104	TLLGIMAHSALSLGLVVVSLM
	178 -> 198	LTIGVAMKFVGALIITSLLII
	54 -> 74	VNPFYAVIAVTLLLAGGLVWL
	236 -> 256	DTPAGPSVVLCAALLFILSMM

Classification:

Multifun Terms (GenProtEC)
		6 - cell structure --> 6.1 - membrane
		4 - transport --> 4.3 - Primary Active Transporters --> 4.3.A - Pyrophosphate Bond (ATP; GTP; P2) Hydrolysis-driven Active Transporters --> 4.3.A.1 - The ATP-binding Cassette (ABC) Superfamily + ABC-type Uptake Permeases --> 4.3.A.1.m - ABC superfamily, membrane component
Gene Ontology Terms (GO)
	cellular_component	GO:0016020 - membrane GO:0005886 - plasma membrane GO:0005887 - integral component of plasma membrane GO:0016021 - integral component of membrane GO:0043190 - ATP-binding cassette (ABC) transporter complex
	molecular_function	GO:0000006 - high-affinity zinc transmembrane transporter activity GO:0042626 - ATPase-coupled transmembrane transporter activity
	biological_process	GO:0071578 - zinc ion import across plasma membrane GO:0006811 - ion transport GO:0006829 - zinc ion transport GO:0055085 - transmembrane transport GO:0010043 - response to zinc ion

Note(s):

Note(s): ...[more].

External database links:

ECOCYC:

ECOLIWIKI:

INTERPRO:

INTERPRO:

MODBASE:

PANTHER:

PFAM:

PRIDE:

PRODB:

REFSEQ:

UNIPROT:

Operon

Name:

znuCB

Operon arrangement:

Transcription unit	Promoter
znuCB	znuCp

Transcriptional Regulation

Display Regulation

Activated by:

OxyR

Repressed by:

Zur

Growth Conditions:

[1]	C: Escherichia coli\| znuB knockout mutant\| MacConkey agar\| (NH4)2Fe(SO4)2 30 µM\| 30.0 C E: Escherichia coli\| znuB knockout mutant\| MacConkey agar\| (NH4)2Fe(SO4)2 30 µM; zinc sulfate 1 mM\| 30.0 C
[2]	C: Escherichia coli\| znuB knockout mutant\| MacConkey agar\| 30.0 C E: Escherichia coli\| znuB knockout mutant\| MacConkey agar\| MnCl2 1 mM\| 30.0 C
[3]	C: Escherichia coli\| znuB knockout mutant\| MacConkey agar\| 30.0 C E: Escherichia coli\| znuB knockout mutant\| MacConkey agar\| (NH4)2Fe(SO4)2 10 mM\| 30.0 C
[4]	C: Escherichia coli\| znuB knockout mutant\| MacConkey agar\| (NH4)2Fe(SO4)2 30 µM\| 30.0 C E: Escherichia coli\| znuB knockout mutant\| MacConkey agar\| (NH4)2Fe(SO4)2 30 µM; desferrioxamine B mesylate 20 mM\| 30.0 C
[5]	C: Escherichia coli\| znuB knockout mutant\| MacConkey agar\| 30.0 C E: Escherichia coli\| znuB knockout mutant\| MacConkey agar\| EGTA 50 mM\| 30.0 C

Elements in the selected gene context region unrelated to any object in RegulonDB

Type	Name	Post Left	Strand	Notes	Evidence (Confirmed, Strong, Weak)	References
promoter	yebAp1	1941683	reverse	Similarity to the consensus sequence of the set of known functional promoters for this sigma factor with strong experimental evidence: good homology. Score: 2.696. P-value: 4.067e-5 Read more >	[ICWHO]	[1]
promoter	yebAp6	1941721	reverse	Similarity to the consensus sequence of the set of known functional promoters for this sigma factor with strong experimental evidence: high homology. Score: 7.3. P-value: 8.918e-7 Read more >	[ICWHO]	[1]
promoter	yebAp5	1941833	reverse	Similarity to the consensus sequence of the set of known functional promoters for this sigma factor with strong experimental evidence: good homology. Score: 5.556. P-value: 2.467e-5 Read more >	[ICWHO]	[1]

Evidence
[ICWHO] Inferred computationally without human oversight

Reference(s)
[1] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78