RegulonDB RegulonDB 11.1: Gene Form
   

lpxH gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

lpxH purE ppiB purK PurR TSS_675 TSS_675 TSS_674 (cluster) TSS_674 (cluster) purEp purEp

Gene      
Name: lpxH    Texpresso search in the literature
Synonym(s): ECK0517, EG12666, b0524, ybbF
Genome position(nucleotides): 553218 <-- 553940
Strand: reverse
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
 54.5
External database links:  
ASAP:
 ABE-0001804
ECHOBASE:
 EB2532
ECOLIHUB:
 lpxH
OU-MICROARRAY:
 b0524
STRING:
 511145.b0524
COLOMBOS: lpxH


Product      
Name: UDP-2,3-diacylglucosamine diphosphatase
Synonym(s): LpxH, YbbF
Sequence: Get amino acid sequence Fasta Format
Cellular location: inner membrane,cytosol
Molecular weight: 26.894
Isoelectric point: 7.761
Motif(s):
 
Type Positions Sequence Comment
1 -> 203 MATLFIADLHLCVEEPAITAGFLRFLAGEARKADALYILGDLFEAWIGDDDPNPLHRKMAAAIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKVLELYGRRVLIMHGDTLCTDDAGYQAFRAKVHKPWLQTLFLALPLFVRKRIAARMRANSKEANSSKSLAIMDVNQNAVVSAMEKHQVQWLIHGHTHRPAVHE
79 -> 80 NR UniProt: Substrate binding; Sequence Annotation Type: region of interest.

 
Classification:
Multifun Terms (GenProtEC)  
  1 - metabolism --> 1.6 - biosynthesis of macromolecules (cellular constituents) --> 1.6.3 - lipopolysaccharide --> 1.6.3.3 - lipid A
  6 - cell structure --> 6.3 - surface antigens (ECA, O antigen of LPS)
Gene Ontology Terms (GO)  
cellular_component GO:0005737 - cytoplasm
GO:0005829 - cytosol
GO:0016020 - membrane
GO:0019897 - extrinsic component of plasma membrane
GO:0005886 - plasma membrane
GO:0031234 - extrinsic component of cytoplasmic side of plasma membrane
molecular_function GO:0016787 - hydrolase activity
GO:0016462 - pyrophosphatase activity
GO:0046872 - metal ion binding
GO:0008758 - UDP-2,3-diacylglucosamine hydrolase activity
GO:0030145 - manganese ion binding
biological_process GO:0006629 - lipid metabolic process
GO:0009245 - lipid A biosynthetic process
Note(s): Note(s): ...[more].
Reference(s): [1] Takayama K., et al., 1983
External database links:  
ALPHAFOLD:
 P43341
DIP:
 DIP-10125N
ECOCYC:
 EG12666-MONOMER
ECOLIWIKI:
 b0524
INTERPRO:
 IPR024654
INTERPRO:
 IPR043461
INTERPRO:
 IPR010138
MODBASE:
 P43341
PANTHER:
 PTHR34990:SF1
PANTHER:
 PTHR34990
PDB:
 5WLY
PFAM:
 PF12850
PRIDE:
 P43341
PRODB:
 PRO_000023124
REFSEQ:
 NP_415057
SMR:
 P43341
UNIPROT:
 P43341


Operon      
Name: ppiB-lpxH         
Operon arrangement:
Transcription unit        Promoter
ppiB-lpxH


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter TSS_674 (cluster) 554360 reverse nd [RS-EPT-CBR] [2]
  promoter TSS_675 554367 reverse nd [RS-EPT-CBR] [2]


Evidence    

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates


Reference(s)    

 [1] Takayama K., Qureshi N., Mascagni P., Nashed MA., Anderson L., Raetz CR., 1983, Fatty acyl derivatives of glucosamine 1-phosphate in Escherichia coli and their relation to lipid A. Complete structure of A diacyl GlcN-1-P found in a phosphatidylglycerol-deficient mutant., J Biol Chem 258(12):7379-85

 [2] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

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