RegulonDB RegulonDB 10.8: Gene Form
   

diaA gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

diaA yraP yraN lpoA yraPp yraPp yraPp2 yraPp2 TSS_3418 TSS_3418 TSS_3417 TSS_3417 TSS_3416 TSS_3416 TSS_3415 TSS_3415

Gene      
Name: diaA    Texpresso search in the literature
Synonym(s): ECK3137, EG12780, b3149, yraO
Genome position(nucleotides): 3295809 --> 3296399 Genome Browser
Strand: forward
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
50.25
External database links:  
ASAP:
ABE-0010348
ECHOBASE:
EB2633
OU-MICROARRAY:
b3149
PortEco:
diaA
STRING:
511145.b3149
COLOMBOS: diaA


Product      
Name: DnaA initiator-associating factor for replication initiation
Synonym(s): DiaA, YraO
Sequence: Get amino acid sequence Fasta Format
Cellular location: cytosol
Molecular weight: 21.106
Isoelectric point: 5.219
Motif(s):
 
Type Positions Sequence
72 -> 72 P
191 -> 191 F
9 -> 144 FTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKILCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQVRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTG
101 -> 101 K
190 -> 191 LF

 

Classification:
Multifun Terms (GenProtEC)  
  2 - information transfer --> 2.1 - DNA related --> 2.1.1 - DNA replication
  5 - cell processes --> 5.2 - cell cycle physiology
Gene Ontology Terms (GO)  
cellular_component GO:0005829 - cytosol
molecular_function GO:0005515 - protein binding
GO:0042802 - identical protein binding
GO:0042803 - protein homodimerization activity
GO:0097367 - carbohydrate derivative binding
biological_process GO:0006260 - DNA replication
GO:1901135 - carbohydrate derivative metabolic process
GO:0032298 - positive regulation of DNA-dependent DNA replication initiation
Note(s): Note(s): ...[more].
Reference(s): [1] Fujimitsu K., et al., 2008
[2] Molt KL., et al., 2009
[3] Natrajan G., et al., 2007
[4] Zawilak-Pawlik A., et al., 2011
External database links:  
DIP:
DIP-35981N
ECOCYC:
YRAO-MONOMER
ECOLIWIKI:
b3149
INTERPRO:
IPR001347
INTERPRO:
IPR035461
INTERPRO:
IPR023070
MODBASE:
P66817
PDB:
4U6N
PDB:
2YVA
PFAM:
PF13580
PRIDE:
P66817
PRODB:
PRO_000022442
PROSITE:
PS51464
REFSEQ:
NP_417618
SMR:
P66817
SWISSMODEL:
P66817
UNIPROT:
P66817


Operon      
Name: diaA         
Operon arrangement:
Transcription unit        Promoter
diaA


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter TSS_3415 3295433 forward nd [RS-EPT-CBR] [5]
  promoter TSS_3416 3295436 forward nd [RS-EPT-CBR] [5]
  promoter TSS_3417 3295444 forward nd [RS-EPT-CBR] [5]
  promoter TSS_3418 3295942 forward nd [RS-EPT-CBR] [5]


Evidence    

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates



Reference(s)    

 [1] Fujimitsu K., Su'etsugu M., Yamaguchi Y., Mazda K., Fu N., Kawakami H., Katayama T., 2008, Modes of overinitiation, dnaA gene expression, and inhibition of cell division in a novel cold-sensitive hda mutant of Escherichia coli., J Bacteriol 190(15):5368-81

 [2] Molt KL., Sutera VA., Moore KK., Lovett ST., 2009, A role for nonessential domain II of initiator protein, DnaA, in replication control., Genetics 183(1):39-49

 [3] Natrajan G., Hall DR., Thompson AC., Gutsche I., Terradot L., 2007, Structural similarity between the DnaA-binding proteins HobA (HP1230) from Helicobacter pylori and DiaA from Escherichia coli., Mol Microbiol 65(4):995-1005

 [4] Zawilak-Pawlik A., Donczew R., Szafranski S., Mackiewicz P., Terradot L., Zakrzewska-Czerwinska J., 2011, DiaA/HobA and DnaA: a pair of proteins co-evolved to cooperate during bacterial orisome assembly., J Mol Biol 408(2):238-51

 [5] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.


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