RegulonDB

Gene
Name:	yrbG
Synonym(s):	ECK3185, EG12802, b3196
Genome position(nucleotides):	3340275 --> 3341252 Genome Browser
Strand:	forward
Sequence:	Get nucleotide sequence FastaFormat
GC content %:	52.04
External database links:

ASAP:	ABE-0010502
ECHOBASE:	EB2654
ECOLIHUB:	yrbG
OU-MICROARRAY:	b3196
STRING:	511145.b3196
COLOMBOS:	yrbG

Product

Name:

putative transport protein YrbG

Synonym(s):

YrbG

Sequence:

Get amino acid sequence Fasta Format

Cellular location:

inner membrane

Molecular weight:

34.741

Isoelectric point:

5.296

Motif(s):

Type	Positions	Sequence
	170 -> 190	GGLPVAFLWLGIALIIMPVAT
	175 -> 317	AFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMGLTAIAIGTSLPELATAIAGVRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIDPLAYSRDYSVMLLVSIIFALLCWRRSPQPGRGVGVLLTGGFIVWLAMLY
	6 -> 26	ALLIVGLLLVVYSADRLVFAA
	270 -> 290	LAYSRDYSVMLLVSIIFALLC
	303 -> 323	VLLTGGFIVWLAMLYWLSPIL

Classification:

Multifun Terms (GenProtEC)
		4 - transport --> 4.2 - Electrochemical potential driven transporters --> 4.2.A - Porters (Uni-, Sym- and Antiporters)
		6 - cell structure --> 6.1 - membrane
Gene Ontology Terms (GO)
	cellular_component	GO:0016020 - membrane GO:0005886 - plasma membrane GO:0005887 - integral component of plasma membrane GO:0016021 - integral component of membrane
	molecular_function	GO:0022857 - transmembrane transporter activity GO:0005262 - calcium channel activity GO:0008273 - calcium, potassium:sodium antiporter activity
	biological_process	GO:0006874 - cellular calcium ion homeostasis GO:0055085 - transmembrane transport GO:0035725 - sodium ion transmembrane transport GO:0071805 - potassium ion transmembrane transport GO:0098656 - anion transmembrane transport GO:0070588 - calcium ion transmembrane transport

Note(s):

Note(s): ...[more].

Reference(s):

[1] Hsieh JM., et al., 2010
[2] Jindal S., et al., 2019

External database links:

ECOCYC:

ECOLIWIKI:

INTERPRO:

INTERPRO:

PANTHER:

PFAM:

PRIDE:

REFSEQ:

UNIPROT:

Operon

Name:

yrbG-kdsDC-lptCAB-rpoN-hpf-ptsN-rapZ-npr

Operon arrangement:

Transcription unit	Promoter
yrbG-kdsD	yrbGp
yrbG-kdsDC-lptCAB	yrbGp
kdsC-lptCAB	kdsCp3
kdsC-lptCAB	kdsCp2
kdsC-lptCAB	kdsCp1
lptAB	lptAp2
lptAB	lptAp1
lptB-rpoN-hpf-ptsN-yhbJ	lptBp
rpoN-hpf-ptsN-yhbJ-npr	rpoNp

RNA cis-regulatory element

Attenuation:	Transcriptional

Elements in the selected gene context region unrelated to any object in RegulonDB

Type	Name	Post Left	Strand	Notes	Evidence (Confirmed, Strong, Weak)	References
promoter	TSS_3505	3341124	forward	nd	[RS-EPT-CBR]	[3]
promoter	TSS_3506	3341718	forward	nd	[RS-EPT-CBR]	[3]
promoter	TSS_3508	3342094	forward	nd	[RS-EPT-CBR]	[3]

Evidence
[RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates

Reference(s)
[1] Hsieh JM., Besserer GM., Madej MG., Bui HQ., Kwon S., Abramson J., 2010, Bridging the gap: a GFP-based strategy for overexpression and purification of membrane proteins with intra and extracellular C-termini., Protein Sci 19(4):868-80
[2] Jindal S., Yang L., Day PJ., Kell DB., 2019, Involvement of multiple influx and efflux transporters in the accumulation of cationic fluorescent dyes by Escherichia coli., BMC Microbiol 19(1):195
[3] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.