RegulonDB RegulonDB 11.0: Gene Form
   

cas2 gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

iap cas1 cas2 ygbFp ygbFp

Gene      
Name: cas2    Texpresso search in the literature
Synonym(s): ECK2749, EG12845, b2754, ygbF
Genome position(nucleotides): 2878569 <-- 2878853
Strand: reverse
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
 46.32
External database links:  
ASAP:
 ABE-0009036
ECHOBASE:
 EB2694
ECOLIHUB:
 cas2
OU-MICROARRAY:
 b2754
STRING:
 511145.b2754
COLOMBOS: cas2


Product      
Name: CRISPR-associated endoribonuclease Cas2
Synonym(s): Cas2, YgbF
Sequence: Get amino acid sequence Fasta Format
Cellular location: cytosol
Molecular weight: 10.518
Isoelectric point: 4.724
Motif(s):
 
Type Positions Sequence Comment
3 -> 88 MLVVVTENVPPRLRGRLAIWLLEVRAGVYVGDVSAKIREMIWEQIAGLAEEGNVVMAWATNTETGFEFQTFGLNRRTPVDLDGLRL
9 -> 9 E UniProt: No effect on spacer acquisition, Cas1-Cas2 complex formation or CRISPR DNA-binding by complex..
10 -> 10 N UniProt: No effect on spacer acquisition..
14 -> 14 R UniProt: Slight decrease in spacer acquisition..
16 -> 16 R UniProt: Slight decrease in spacer acquisition..

 
Classification:
Multifun Terms (GenProtEC)  
  5 - cell processes --> 5.10 - defense/survival
Gene Ontology Terms (GO)  
cellular_component GO:0005829 - cytosol
molecular_function GO:0003677 - DNA binding
GO:0004518 - nuclease activity
GO:0004519 - endonuclease activity
GO:0005515 - protein binding
GO:0016787 - hydrolase activity
biological_process GO:0099048 - CRISPR-cas system
GO:0051607 - defense response to virus
GO:0090305 - nucleic acid phosphodiester bond hydrolysis
GO:0043571 - maintenance of CRISPR repeat elements
External database links:  
ALPHAFOLD:
 P45956
DIP:
 DIP-12109N
ECOCYC:
 EG12845-MONOMER
ECOLIWIKI:
 b2754
INTERPRO:
 IPR010152
PDB:
 5DS4
PDB:
 5WFE
PDB:
 5VVL
PDB:
 5VVK
PDB:
 5VVJ
PDB:
 4MAK
PDB:
 4P6I
PDB:
 4QDL
PDB:
 5DLJ
PDB:
 5DQT
PDB:
 5DQU
PDB:
 5DQZ
PDB:
 5DS5
PDB:
 5DS6
PFAM:
 PF09707
PRIDE:
 P45956
REFSEQ:
 NP_417234
SMR:
 P45956
UNIPROT:
 P45956


Operon      
Name: casABCDE12         
Operon arrangement:
Transcription unit        Promoter
ygbF
casABCDE12
casDE-ygbTF


Transcriptional Regulation      
Display Regulation             
Activated by: SlyA, LeuO, StpA
Repressed by: CRP, H-NS


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References



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