RegulonDB RegulonDB 11.0: Gene Form
   

yajI gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

tsx yajI nrdR DeoR CRP CytR CytR CRP CytR yajIp6 yajIp6 nrdRp nrdRp TSS_526 TSS_526 tsxp1 tsxp1 TSS_525 TSS_525 tsxp2 tsxp2 TSS_524 TSS_524

Gene      
Name: yajI    Texpresso search in the literature
Synonym(s): ECK0406, EG12874, b0412
Genome position(nucleotides): 432312 <-- 432851
Strand: reverse
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
 47.59
External database links:  
ASAP:
 ABE-0001433
ECHOBASE:
 EB2712
ECOLIHUB:
 yajI
OU-MICROARRAY:
 b0412
STRING:
 511145.b0412
COLOMBOS: yajI


Product      
Name: putative lipoprotein YajI
Synonym(s): YajI
Sequence: Get amino acid sequence Fasta Format
Cellular location: inner membrane,periplasmic space
Molecular weight: 19.56
Isoelectric point: 5.13
Motif(s):
 
Type Positions Sequence Comment
1 -> 19 MNTNVFRLLLLGSLFSLSA
20 -> 176 CVQQSEVRQMKHSVSTLNQEMTQLNQETVKITQQNRLNAKSSSGVYLLPGAKTPARLESQIGTLRMSLVNITPDADGTTLTLRIQGESNDPLPAFSGTVEYGQIQGTIDNFQEINVQNQLINAPASVLAPSDVDIPLQLKGISVDQLGFVRIHDIQP

 
Classification:
Multifun Terms (GenProtEC)  
Gene Ontology Terms (GO)  
cellular_component GO:0016020 - membrane
GO:0030288 - outer membrane-bounded periplasmic space
GO:0005886 - plasma membrane
Note(s): Note(s): ...[more].
Reference(s): [1] Rao S., et al., 2020
External database links:  
ALPHAFOLD:
 P46122
ECOCYC:
 EG12874-MONOMER
ECOLIWIKI:
 b0412
INTERPRO:
 IPR037125
INTERPRO:
 IPR021658
PDB:
 2JWY
PFAM:
 PF11622
PRIDE:
 P46122
PROSITE:
 PS51257
REFSEQ:
 NP_414946
SMR:
 P46122
UNIPROT:
 P46122


Operon      
Name: yajI         
Operon arrangement:
Transcription unit        Promoter
yajI


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter TSS_524 432002 reverse nd [RS-EPT-CBR] [2]
  promoter TSS_525 432185 reverse nd [RS-EPT-CBR] [2]
  promoter TSS_526 432975 forward nd [RS-EPT-CBR] [2]
  promoter yajIp6 433034 reverse nd [ICWHO] [3]


Evidence    

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates

 [ICWHO] Inferred computationally without human oversight


Reference(s)    

 [1] Rao S., Bates GT., Matthews CR., Newport TD., Vickery ON., Stansfeld PJ., 2020, Characterizing Membrane Association and Periplasmic Transfer of Bacterial Lipoproteins through Molecular Dynamics Simulations., Structure 28(4):475-487.e3

 [2] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

 [3] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78

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