RegulonDB

Gene
Name:	aqpZ
Synonym(s):	ECK0866, EG13270, b0875, bniP
Genome position(nucleotides):	915352 <-- 916047
Strand:	reverse
Sequence:	Get nucleotide sequence FastaFormat
GC content %:	52.3
External database links:

ASAP:	ABE-0002976
ECHOBASE:	EB3055
ECOLIHUB:	aqpZ
MIM:	110450
OU-MICROARRAY:	b0875
STRING:	511145.b0875
COLOMBOS:	aqpZ

Gene

Name:

aqpZ

Synonym(s):

ECK0866, EG13270, b0875, bniP

Genome position(nucleotides):

915352 <-- 916047

Strand:

reverse

Sequence:

Get nucleotide sequence FastaFormat

GC content %:

52.3

External database links:

ASAP:

ECHOBASE:

ECOLIHUB:

MIM:

OU-MICROARRAY:

STRING:

COLOMBOS:

Product

Name:

water channel AqpZ

Synonym(s):

AqpZ, BniP

Sequence:

Get amino acid sequence Fasta Format

Cellular location:

inner membrane

Molecular weight:

23.703

Isoelectric point:

7.629

Motif(s):

Type	Positions	Sequence	Comment
	1 -> 26	MFRKLAAECFGTFWLVFGGCGSAVLA
	2 -> 223	FRKLAAECFGTFWLVFGGCGSAVLAAGFPELGIGFAGVALAFGLTVLTMAFAVGHISGGHFNPAVTIGLWAGGRFPAKEVVGYVIAQVVGGIVAAALLYLIASGKTGFDAAASGFASNGYGEHSPGGYSMLSALVVELVLSAGFLLVIHGATDKFAPAGFAPIAIGLALTLIHLISIPVTNTSVNPARSTAVAIFQGGWALEQLWFFWVVPIVGGIIGGLIY
	9 -> 9	C	UniProt: No effect..
	14 -> 14	W	UniProt: In Ref. 2; BAA08441..
	20 -> 20	C	UniProt: Loss of oligomerization; no alteration of water permeability..

Classification:

Multifun Terms (GenProtEC)
		4 - transport --> 4.1 - Channel-type Transporters --> 4.1.A - alpha-type channels
		5 - cell processes --> 5.5 - adaptations --> 5.5.1 - osmotic pressure
		6 - cell structure --> 6.1 - membrane
Gene Ontology Terms (GO)
	cellular_component	GO:0016020 - membrane GO:0005886 - plasma membrane GO:0005887 - integral component of plasma membrane GO:0016021 - integral component of membrane
	molecular_function	GO:0005515 - protein binding GO:0015267 - channel activity GO:0015250 - water channel activity GO:0042802 - identical protein binding
	biological_process	GO:0006970 - response to osmotic stress GO:0009992 - cellular water homeostasis GO:0006833 - water transport GO:0055085 - transmembrane transport

Note(s):

Note(s): ...[more].

Reference(s):

[1] Borgnia MJ., et al., 2001
[2] Calamita G., et al., 1997
[3] Fushimi K., et al., 1997
[4] Hashido M., et al., 2005
[5] Hu G., et al., 2012
[6] Jensen MO., et al., 2006
[7] Mallo RC., et al., 2006
[8] Mohanty AK., et al., 2004
[9] Pohl P., et al., 2001
[10] Savage DF., et al., 2010
[11] Scheuring S., et al., 2002
[12] Scheuring S., et al., 2000
[13] Sipe SN., et al., 2020
[14] Stroud RM., et al., 2003
[15] Tan H., et al., 2021
[16] Wang Y., et al., 2005
[17] Xie H., et al., 2018
[18] Yue K., et al., 2019

External database links:

ALPHAFOLD:

DIP:

ECOCYC:

ECOLIWIKI:

INTERPRO:

INTERPRO:

INTERPRO:

INTERPRO:

INTERPRO:

MODBASE:

PANTHER:

PDB:

PDB:

PDB:

PDB:

PDB:

PDB:

PDB:

PDB:

PDB:

PFAM:

PRIDE:

PRINTS:

PRODB:

PROSITE:

REFSEQ:

SMR:

UNIPROT:

Type	Name	Post Left	Strand	Notes	Evidence (Confirmed, Strong, Weak)	References
promoter	ybjEp3	914901	reverse	nd	[COMP-AINF]	[19]
promoter	ybjEp5	915077	reverse	nd	[COMP-AINF]	[19]
promoter	aqpZp3	916133	reverse	nd	[COMP-AINF]	[19]
promoter	aqpZp2	916212	reverse	nd	[COMP-AINF]	[19]

Type

Name

Post Left

Post Right

Strand

Notes

Evidence (Confirmed, Strong, Weak)

References

promoter

ybjEp3

914901

reverse

[COMP-AINF]

[19]

promoter

ybjEp5

915077

reverse

[COMP-AINF]

[19]

promoter

aqpZp3

916133

reverse

[COMP-AINF]

[19]

promoter

aqpZp2

916212

reverse

[COMP-AINF]

[19]

Evidence
[COMP-AINF] Inferred computationally without human oversight

Evidence

[COMP-AINF] Inferred computationally without human oversight

Reference(s)
[1] Borgnia MJ., Agre P., 2001, Reconstitution and functional comparison of purified GlpF and AqpZ, the glycerol and water channels from Escherichia coli., Proc Natl Acad Sci U S A 98(5):2888-93
[2] Calamita G., Kempf B., Rudd KE., Bonhivers M., Kneip S., Bishai WR., Bremer E., Agre P., 1997, The aquaporin-Z water channel gene of Escherichia coli: structure, organization and phylogeny., Biol Cell 89(5-6):321-9
[3] Fushimi K., Bai L., Marumo F., Sasaki S., 1997, Isolation of a gene encoding nodulin-like intrinsic protein of Escherichia coli., Biochem Mol Biol Int 41(5):995-1003
[4] Hashido M., Ikeguchi M., Kidera A., 2005, Comparative simulations of aquaporin family: AQP1, AQPZ, AQP0 and GlpF., FEBS Lett 579(25):5549-52
[5] Hu G., Chen LY., Wang J., 2012, Insights into the mechanisms of the selectivity filter of Escherichia coli aquaporin Z., J Mol Model 18(8):3731-41
[6] Jensen MO., Mouritsen OG., 2006, Single-channel water permeabilities of Escherichia coli aquaporins AqpZ and GlpF., Biophys J 90(7):2270-84
[7] Mallo RC., Ashby MT., 2006, AqpZ-mediated water permeability in Escherichia coli measured by stopped-flow spectroscopy., J Bacteriol 188(2):820-2
[8] Mohanty AK., Wiener MC., 2004, Membrane protein expression and production: effects of polyhistidine tag length and position., Protein Expr Purif 33(2):311-25
[9] Pohl P., Saparov SM., Borgnia MJ., Agre P., 2001, Highly selective water channel activity measured by voltage clamp: analysis of planar lipid bilayers reconstituted with purified AqpZ., Proc Natl Acad Sci U S A 98(17):9624-9
[10] Savage DF., O'Connell JD., Miercke LJ., Finer-Moore J., Stroud RM., 2010, Structural context shapes the aquaporin selectivity filter., Proc Natl Acad Sci U S A 107(40):17164-9
[11] Scheuring S., Muller DJ., Stahlberg H., Engel HA., Engel A., 2002, Sampling the conformational space of membrane protein surfaces with the AFM., Eur Biophys J 31(3):172-8
[12] Scheuring S., Tittmann P., Stahlberg H., Ringler P., Borgnia M., Agre P., Gross H., Engel A., 2000, The aquaporin sidedness revisited., J Mol Biol 299(5):1271-8
[13] Sipe SN., Patrick JW., Laganowsky A., Brodbelt JS., 2020, Enhanced Characterization of Membrane Protein Complexes by Ultraviolet Photodissociation Mass Spectrometry., Anal Chem 92(1):899-907
[14] Stroud RM., Savage D., Miercke LJ., Lee JK., Khademi S., Harries W., 2003, Selectivity and conductance among the glycerol and water conducting aquaporin family of channels., FEBS Lett 555(1):79-84
[15] Tan H., Zhao Y., Zhao W., Xie H., Chen Y., Tong Q., Yang J., 2021, Dynamics properties of membrane proteins in native cell membranes revealed by solid-state NMR spectroscopy., Biochim Biophys Acta Biomembr 1864(1):183791
[16] Wang Y., Schulten K., Tajkhorshid E., 2005, What makes an aquaporin a glycerol channel? A comparative study of AqpZ and GlpF., Structure 13(8):1107-18
[17] Xie H., Zhao Y., Wang J., Zhang Z., Yang J., 2018, Solid-state NMR chemical shift assignments of aquaporin Z in lipid bilayers., Biomol NMR Assign 12(2):323-328
[18] Yue K., Trung TN., Zhu Y., Kaldenhoff R., Kai L., 2019, Co-Translational Insertion of Aquaporins into Liposome for Functional Analysis via an E. coli Based Cell-Free Protein Synthesis System., Cells 8(11)
[19] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78

Reference(s)

[1] Borgnia MJ., Agre P., 2001, Reconstitution and functional comparison of purified GlpF and AqpZ, the glycerol and water channels from Escherichia coli., Proc Natl Acad Sci U S A 98(5):2888-93

[2] Calamita G., Kempf B., Rudd KE., Bonhivers M., Kneip S., Bishai WR., Bremer E., Agre P., 1997, The aquaporin-Z water channel gene of Escherichia coli: structure, organization and phylogeny., Biol Cell 89(5-6):321-9

[3] Fushimi K., Bai L., Marumo F., Sasaki S., 1997, Isolation of a gene encoding nodulin-like intrinsic protein of Escherichia coli., Biochem Mol Biol Int 41(5):995-1003

[4] Hashido M., Ikeguchi M., Kidera A., 2005, Comparative simulations of aquaporin family: AQP1, AQPZ, AQP0 and GlpF., FEBS Lett 579(25):5549-52

[5] Hu G., Chen LY., Wang J., 2012, Insights into the mechanisms of the selectivity filter of Escherichia coli aquaporin Z., J Mol Model 18(8):3731-41

[6] Jensen MO., Mouritsen OG., 2006, Single-channel water permeabilities of Escherichia coli aquaporins AqpZ and GlpF., Biophys J 90(7):2270-84

[7] Mallo RC., Ashby MT., 2006, AqpZ-mediated water permeability in Escherichia coli measured by stopped-flow spectroscopy., J Bacteriol 188(2):820-2

[8] Mohanty AK., Wiener MC., 2004, Membrane protein expression and production: effects of polyhistidine tag length and position., Protein Expr Purif 33(2):311-25

[9] Pohl P., Saparov SM., Borgnia MJ., Agre P., 2001, Highly selective water channel activity measured by voltage clamp: analysis of planar lipid bilayers reconstituted with purified AqpZ., Proc Natl Acad Sci U S A 98(17):9624-9

[10] Savage DF., O'Connell JD., Miercke LJ., Finer-Moore J., Stroud RM., 2010, Structural context shapes the aquaporin selectivity filter., Proc Natl Acad Sci U S A 107(40):17164-9

[11] Scheuring S., Muller DJ., Stahlberg H., Engel HA., Engel A., 2002, Sampling the conformational space of membrane protein surfaces with the AFM., Eur Biophys J 31(3):172-8