RegulonDB RegulonDB 10.9: Gene Form
   

aqpZ gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

ybjD lysO aqpZ ybjX ArgR ArgR NarP NarL aqpZp2 aqpZp2 aqpZp3 aqpZp3 ybjEp5 ybjEp5 ybjEp3 ybjEp3 lysOp lysOp hcpp2 hcpp2

Gene      
Name: aqpZ    Texpresso search in the literature
Synonym(s): ECK0866, EG13270, b0875, bniP
Genome position(nucleotides): 915352 <-- 916047 Genome Browser
Strand: reverse
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
52.3
External database links:  
ASAP:
ABE-0002976
ECHOBASE:
EB3055
ECOLIHUB:
aqpZ
MIM:
110450
OU-MICROARRAY:
b0875
STRING:
511145.b0875
COLOMBOS: aqpZ


Product      
Name: water channel AqpZ
Synonym(s): AqpZ, BniP
Sequence: Get amino acid sequence Fasta Format
Cellular location: inner membrane
Molecular weight: 23.703
Isoelectric point: 7.629
Motif(s):
 
Type Positions Sequence
2 -> 223 FRKLAAECFGTFWLVFGGCGSAVLAAGFPELGIGFAGVALAFGLTVLTMAFAVGHISGGHFNPAVTIGLWAGGRFPAKEVVGYVIAQVVGGIVAAALLYLIASGKTGFDAAASGFASNGYGEHSPGGYSMLSALVVELVLSAGFLLVIHGATDKFAPAGFAPIAIGLALTLIHLISIPVTNTSVNPARSTAVAIFQGGWALEQLWFFWVVPIVGGIIGGLIY
34 -> 54 IGFAGVALAFGLTVLTMAFAV
206 -> 229 WFFWVVPIVGGIIGGLIYRTLLEK
26 -> 26 A
79 -> 103 KEVVGYVIAQVVGGIVAAALLYLIA

 

Classification:
Multifun Terms (GenProtEC)  
  4 - transport --> 4.1 - Channel-type Transporters --> 4.1.A - alpha-type channels
  5 - cell processes --> 5.5 - adaptations --> 5.5.1 - osmotic pressure
  6 - cell structure --> 6.1 - membrane
Gene Ontology Terms (GO)  
cellular_component GO:0016020 - membrane
GO:0005886 - plasma membrane
GO:0005887 - integral component of plasma membrane
GO:0016021 - integral component of membrane
molecular_function GO:0005515 - protein binding
GO:0015267 - channel activity
GO:0015250 - water channel activity
GO:0042802 - identical protein binding
biological_process GO:0006970 - response to osmotic stress
GO:0009992 - cellular water homeostasis
GO:0006833 - water transport
GO:0055085 - transmembrane transport
Note(s): Note(s): ...[more].
Reference(s): [1] Borgnia MJ., et al., 2001
[2] Calamita G., et al., 1997
[3] Fushimi K., et al., 1997
[4] Hashido M., et al., 2005
[5] Hu G., et al., 2012
[6] Jensen MO., et al., 2006
[7] Mallo RC., et al., 2006
[8] Mohanty AK., et al., 2004
[9] Pohl P., et al., 2001
[10] Savage DF., et al., 2010
[11] Scheuring S., et al., 2002
[12] Scheuring S., et al., 2000
[13] Sipe SN., et al., 2020
[14] Stroud RM., et al., 2003
[15] Wang Y., et al., 2005
[16] Xie H., et al., 2018
[17] Yue K., et al., 2019
External database links:  
DIP:
DIP-35499N
ECOCYC:
AQPZ-MONOMER
ECOLIWIKI:
b0875
INTERPRO:
IPR023743
INTERPRO:
IPR034294
INTERPRO:
IPR023271
INTERPRO:
IPR022357
INTERPRO:
IPR000425
MODBASE:
P60844
PANTHER:
PTHR45724
PDB:
3NKA
PDB:
3NK5
PDB:
3NKC
PDB:
2abm
PDB:
2o9d
PDB:
2o9e
PDB:
2o9g
PDB:
2o9f
PDB:
1RC2
PFAM:
PF00230
PRIDE:
P60844
PRINTS:
PR00783
PRODB:
PRO_000022113
PROSITE:
PS00221
REFSEQ:
NP_415396
SMR:
P60844
UNIPROT:
P60844


Operon      
Name: aqpZ         
Operon arrangement:
Transcription unit        Promoter
aqpZ


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter ybjEp3 914901 reverse Similarity to the consensus
Read more >
[ICWHO] [18]
  promoter ybjEp5 915077 reverse Similarity to the consensus
Read more >
[ICWHO] [18]
  promoter aqpZp3 916133 reverse Similarity to the consensus
Read more >
[ICWHO] [18]
  promoter aqpZp2 916212 reverse Similarity to the consensus
Read more >
[ICWHO] [18]


Evidence    

 [ICWHO] Inferred computationally without human oversight



Reference(s)    

 [1] Borgnia MJ., Agre P., 2001, Reconstitution and functional comparison of purified GlpF and AqpZ, the glycerol and water channels from Escherichia coli., Proc Natl Acad Sci U S A 98(5):2888-93

 [2] Calamita G., Kempf B., Rudd KE., Bonhivers M., Kneip S., Bishai WR., Bremer E., Agre P., 1997, The aquaporin-Z water channel gene of Escherichia coli: structure, organization and phylogeny., Biol Cell 89(5-6):321-9

 [3] Fushimi K., Bai L., Marumo F., Sasaki S., 1997, Isolation of a gene encoding nodulin-like intrinsic protein of Escherichia coli., Biochem Mol Biol Int 41(5):995-1003

 [4] Hashido M., Ikeguchi M., Kidera A., 2005, Comparative simulations of aquaporin family: AQP1, AQPZ, AQP0 and GlpF., FEBS Lett 579(25):5549-52

 [5] Hu G., Chen LY., Wang J., 2012, Insights into the mechanisms of the selectivity filter of Escherichia coli aquaporin Z., J Mol Model 18(8):3731-41

 [6] Jensen MO., Mouritsen OG., 2006, Single-channel water permeabilities of Escherichia coli aquaporins AqpZ and GlpF., Biophys J 90(7):2270-84

 [7] Mallo RC., Ashby MT., 2006, AqpZ-mediated water permeability in Escherichia coli measured by stopped-flow spectroscopy., J Bacteriol 188(2):820-2

 [8] Mohanty AK., Wiener MC., 2004, Membrane protein expression and production: effects of polyhistidine tag length and position., Protein Expr Purif 33(2):311-25

 [9] Pohl P., Saparov SM., Borgnia MJ., Agre P., 2001, Highly selective water channel activity measured by voltage clamp: analysis of planar lipid bilayers reconstituted with purified AqpZ., Proc Natl Acad Sci U S A 98(17):9624-9

 [10] Savage DF., O'Connell JD., Miercke LJ., Finer-Moore J., Stroud RM., 2010, Structural context shapes the aquaporin selectivity filter., Proc Natl Acad Sci U S A 107(40):17164-9

 [11] Scheuring S., Muller DJ., Stahlberg H., Engel HA., Engel A., 2002, Sampling the conformational space of membrane protein surfaces with the AFM., Eur Biophys J 31(3):172-8

 [12] Scheuring S., Tittmann P., Stahlberg H., Ringler P., Borgnia M., Agre P., Gross H., Engel A., 2000, The aquaporin sidedness revisited., J Mol Biol 299(5):1271-8

 [13] Sipe SN., Patrick JW., Laganowsky A., Brodbelt JS., 2020, Enhanced Characterization of Membrane Protein Complexes by Ultraviolet Photodissociation Mass Spectrometry., Anal Chem 92(1):899-907

 [14] Stroud RM., Savage D., Miercke LJ., Lee JK., Khademi S., Harries W., 2003, Selectivity and conductance among the glycerol and water conducting aquaporin family of channels., FEBS Lett 555(1):79-84

 [15] Wang Y., Schulten K., Tajkhorshid E., 2005, What makes an aquaporin a glycerol channel? A comparative study of AqpZ and GlpF., Structure 13(8):1107-18

 [16] Xie H., Zhao Y., Wang J., Zhang Z., Yang J., 2018, Solid-state NMR chemical shift assignments of aquaporin Z in lipid bilayers., Biomol NMR Assign 12(2):323-328

 [17] Yue K., Trung TN., Zhu Y., Kaldenhoff R., Kai L., 2019, Co-Translational Insertion of Aquaporins into Liposome for Functional Analysis via an E. coli Based Cell-Free Protein Synthesis System., Cells 8(11)

 [18] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78


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