RegulonDB

Gene
Name:	aspV
Synonym(s):	ECK0216, EG30025, b0216
Genome position(nucleotides):	236931 --> 237007 Genome Browser
Strand:	forward
Sequence:	Get nucleotide sequence FastaFormat
GC content %:	63.64
External database links:

ASAP:	ABE-0000724
CGSC:	987
ECHOBASE:	EB4188
ECOLIHUB:	aspV
OU-MICROARRAY:	b0216
COLOMBOS:	aspV

Product

Name:

tRNA-Asp(GUC)

Synonym(s):

tRNA^aspV

Type:

tRNAs

Cellular location:

cytosol

Classification:

Multifun Terms (GenProtEC)
Multifun Terms (GenProtEC)		2 - information transfer --> 2.2 - RNA related --> 2.2.5 - tRNA
Gene Ontology Terms (GO)
	cellular_component	GO:0005737 - cytoplasm GO:0005829 - cytosol
	molecular_function	GO:0030533 - triplet codon-amino acid adaptor activity

Note(s):

Note(s): ...[more].

Evidence:

[TAS] Traceable author statement

Reference(s):

[1] Horiuchi T., et al., 1987
[2] Siekevitz P., et al., 1981

External database links:

ECOCYC:

ECOLIWIKI:

Operon

Name:

aspV

Operon arrangement:

Transcription unit	Promoter
aspV	aspVp

Transcriptional Regulation
	Display Regulation
Activated by:	Fis

Elements in the selected gene context region unrelated to any object in RegulonDB

Type	Name	Post Left	Strand	Notes	Evidence (Confirmed, Strong, Weak)	References
promoter	TSS_435	236931	forward	nd	[RS-EPT-CBR]	[3]
promoter	yafTp4	237235	forward	Similarity to the consensus sequence of the set of known functional promoters for this sigma factor with strong experimental evidence: high homology. Score: 4.38. P-value: 4.586e-5 Read more >	[ICWHO]	[4]
promoter	yafTp5	237260	forward	Similarity to the consensus sequence of the set of known functional promoters for this sigma factor with strong experimental evidence: high homology. Score: 4.63. P-value: 2.953e-5 Read more >	[ICWHO]	[4]
promoter	yafTp6	237278	forward	Similarity to the consensus sequence of the set of known functional promoters for this sigma factor with strong experimental evidence: high homology. Score: 5.143. P-value: 4.330e-6 Read more >	[ICWHO]	[4]

Evidence
[RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates
[ICWHO] Inferred computationally without human oversight

Reference(s)
[1] Horiuchi T., Nagasawa T., Takano K., Sekiguchi M., 1987, A newly discovered tRNA(1Asp) gene (aspV) of Escherichia coli K12., Mol Gen Genet 206(2):356-7
[2] Siekevitz P., Zamecnik PC., 1981, Ribosomes and protein synthesis., J Cell Biol 91(3 Pt 2):53s-65s
[3] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.
[4] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78