RegulonDB

Gene
Name:	fadE
Synonym(s):	ECK0222, G6105, b0221, yafH
Genome position(nucleotides):	240859 <-- 243303 Genome Browser
Strand:	reverse
Sequence:	Get nucleotide sequence FastaFormat
GC content %:	56.89
External database links:

ASAP:	ABE-0000743
ECHOBASE:	EB2939
ECOLIHUB:	fadE
OU-MICROARRAY:	b0221
STRING:	511145.b0221
COLOMBOS:	fadE

Product

Name:

acyl-CoA dehydrogenase

Synonym(s):

FadE, YafH

Sequence:

Get amino acid sequence Fasta Format

Cellular location:

cytosol,inner membrane

Molecular weight:

89.224

Isoelectric point:

8.214

Motif(s):

Type	Positions	Sequence
	514 -> 792	IRCHPYVLEEMEAAKNNDVNAFDKLLFKHIGHVGSNKVRSFWLGLTRGLTSSTPTGDATKRYYQHLNRLSANLALLSDVSMAVLGGSLKRRERISARLGDILSQLYLASAVLKRYDDEGRNEADLPLVHWGVQDALYQAEQAMDDLLQNFPNRVVAGLLNVVIFPTGRHYLAPSDKLDHKVAKILQVPNATRSRIGRGQYLTPSEHNPVGLLEEALVDVIAADPIHQRICKELGKNLPFTRLDELAHNALVKGLIDKDEAAILVKAEESRLRSINVDDF
	147 -> 232	ELADLPPELWAYLKEHRFFAMIIKKEYGGLEFSAYAQSRVLQKLSGVSGILAITVGVPNSLGPGELLQHYGTDEQKDHYLPRLARG
	360 -> 504	GQGWRMLVECLSVGRGITLPSNSTGGVKSVALATGAYAHIRRQFKISIGKMEGIEEPLARIAGNAYVMDAAASLITYGIMLGEKPAVLSAIVKYHCTHRGQQSIIDAMDITGGKGIMLGQSNFLARAYQGAPIAITVEGANILTR
	237 -> 328	CFALTSPEAGSDAGAIPDTGIVCMGEWQGQQVLGMRLTWNKRYITLAPIATVLGLAFKLSDPEKLLGGAEDLGITCALIPTTTPGVEIGRRH

Classification:

Multifun Terms (GenProtEC)
Multifun Terms (GenProtEC)		1 - metabolism --> 1.1 - carbon utilization --> 1.1.2 - fatty acids
Gene Ontology Terms (GO)
	cellular_component	GO:0005737 - cytoplasm GO:0005886 - plasma membrane
	molecular_function	GO:0004466 - long-chain-acyl-CoA dehydrogenase activity GO:0016491 - oxidoreductase activity GO:0016627 - oxidoreductase activity, acting on the CH-CH group of donors GO:0050660 - flavin adenine dinucleotide binding GO:0003995 - acyl-CoA dehydrogenase activity
	biological_process	GO:0006629 - lipid metabolic process GO:0006631 - fatty acid metabolic process GO:0009062 - fatty acid catabolic process GO:0006635 - fatty acid beta-oxidation GO:0033539 - fatty acid beta-oxidation using acyl-CoA dehydrogenase

Note(s):

Note(s): ...[more].

Reference(s):

[1] Clomburg JM., et al., 2012
[2] Cronan JE., et al., 1975
[3] Gulevich AY., et al., 2016
[4] Harley JB., et al., 1978
[5] Iram SH., et al., 2006
[6] Lau C., et al., 1983
[7] Lu X., et al., 2003
[8] Olsen WL., et al., 1979

External database links:

ECOCYC:

ACYLCOADEHYDROG-MONOMER

ECOLIWIKI:

INTERPRO:

INTERPRO:

INTERPRO:

INTERPRO:

INTERPRO:

INTERPRO:

INTERPRO:

MODBASE:

PFAM:

PFAM:

PFAM:

PFAM:

PRIDE:

PRODB:

PROSITE:

REFSEQ:

UNIPROT:

Operon

Name:

fadE

Operon arrangement:

Transcription unit	Promoter
fadE	fadEp

Transcriptional Regulation
	Display Regulation
Repressed by:	FadR, ArcA

Elements in the selected gene context region unrelated to any object in RegulonDB

Type	Name	Post Left	Strand	Notes	Evidence (Confirmed, Strong, Weak)	References
promoter	lpcAp1	243468	forward	nd	[ICWHO]	[9]
promoter	TSS_437	243512	forward	nd	[RS-EPT-CBR]	[10]
promoter	TSS_438	244083	forward	nd	[RS-EPT-CBR]	[10]
promoter	TSS_439	244089	forward	nd	[RS-EPT-CBR]	[10]
promoter	TSS_440 (cluster)	244094	forward	nd	[RS-EPT-CBR]	[10]
promoter	TSS_441	244103	forward	nd	[RS-EPT-CBR]	[10]
promoter	TSS_442	244121	forward	nd	[RS-EPT-CBR]	[10]

Evidence
[ICWHO] Inferred computationally without human oversight
[RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates

Reference(s)
[1] Clomburg JM., Vick JE., Blankschien MD., Rodriguez-Moya M., Gonzalez R., 2012, A synthetic biology approach to engineer a functional reversal of the β-oxidation cycle., ACS Synth Biol 1(11):541-54
[2] Cronan JE., Weisberg LJ., Allen RG., 1975, Regulation of membrane lipid synthesis in Escherichia coli. Accumulation of free fatty acids of abnormal length during inhibition of phospholipid synthesis., J Biol Chem 250(15):5835-40
[3] Gulevich AY., Skorokhodova AY., Debabov VG., 2016, [Escherichia coli ydiO and ydiQRST genes encode components of acyl-CoA dehydrogenase complex of anaerobic fatty acid β-oxidation pathway]., Genetika 52(10):1210-4
[4] Harley JB., Santangelo GM., Rasmussen H., Goldfine H., 1978, Dependence of Escherichia coli hyperbaric oxygen toxicity on the lipid acyl chain composition., J Bacteriol 134(3):808-20
[5] Iram SH., Cronan JE., 2006, The beta-oxidation systems of Escherichia coli and Salmonella enterica are not functionally equivalent., J Bacteriol 188(2):599-608
[6] Lau C., Greenway DL., Freter CE., Neungton N., Wattenberg B., Silbert DF., 1983, Conditions influencing formation of 16:0/16:0 molecular species in membrane phospholipids of Escherichia coli., J Biol Chem 258(21):13027-33
[7] Lu X., Zhang J., Wu Q., Chen GQ., 2003, Enhanced production of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) via manipulating the fatty acid beta-oxidation pathway in E. coli., FEMS Microbiol Lett 221(1):97-101
[8] Olsen WL., Schaechter M., Khorana HG., 1979, Incorporation of synthetic fatty acid analogs into phospholipids of Escherichia coli., J Bacteriol 137(3):1443-6
[9] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78
[10] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.