RegulonDB

Gene
Name:	fadE
Synonym(s):	ECK0222, G6105, b0221, yafH
Genome position(nucleotides):	240859 <-- 243303 Genome Browser
Strand:	reverse
Sequence:	Get nucleotide sequence FastaFormat
GC content %:	56.89
External database links:

ASAP:	ABE-0000743
ECHOBASE:	EB2939
ECOLIHUB:	fadE
OU-MICROARRAY:	b0221
STRING:	511145.b0221
COLOMBOS:	fadE

Product

Name:

acyl-CoA dehydrogenase

Synonym(s):

FadE, YafH

Sequence:

Get amino acid sequence Fasta Format

Cellular location:

cytosol,inner membrane

Molecular weight:

89.224

Isoelectric point:

8.214

Motif(s):

Type	Positions	Sequence
	514 -> 792	IRCHPYVLEEMEAAKNNDVNAFDKLLFKHIGHVGSNKVRSFWLGLTRGLTSSTPTGDATKRYYQHLNRLSANLALLSDVSMAVLGGSLKRRERISARLGDILSQLYLASAVLKRYDDEGRNEADLPLVHWGVQDALYQAEQAMDDLLQNFPNRVVAGLLNVVIFPTGRHYLAPSDKLDHKVAKILQVPNATRSRIGRGQYLTPSEHNPVGLLEEALVDVIAADPIHQRICKELGKNLPFTRLDELAHNALVKGLIDKDEAAILVKAEESRLRSINVDDF
	147 -> 232	ELADLPPELWAYLKEHRFFAMIIKKEYGGLEFSAYAQSRVLQKLSGVSGILAITVGVPNSLGPGELLQHYGTDEQKDHYLPRLARG
	360 -> 504	GQGWRMLVECLSVGRGITLPSNSTGGVKSVALATGAYAHIRRQFKISIGKMEGIEEPLARIAGNAYVMDAAASLITYGIMLGEKPAVLSAIVKYHCTHRGQQSIIDAMDITGGKGIMLGQSNFLARAYQGAPIAITVEGANILTR
	237 -> 328	CFALTSPEAGSDAGAIPDTGIVCMGEWQGQQVLGMRLTWNKRYITLAPIATVLGLAFKLSDPEKLLGGAEDLGITCALIPTTTPGVEIGRRH

Classification:

Multifun Terms (GenProtEC)
Multifun Terms (GenProtEC)		1 - metabolism --> 1.1 - carbon utilization --> 1.1.2 - fatty acids
Gene Ontology Terms (GO)
	cellular_component	GO:0005737 - cytoplasm GO:0005886 - plasma membrane
	molecular_function	GO:0004466 - long-chain-acyl-CoA dehydrogenase activity GO:0016491 - oxidoreductase activity GO:0016627 - oxidoreductase activity, acting on the CH-CH group of donors GO:0050660 - flavin adenine dinucleotide binding GO:0003995 - acyl-CoA dehydrogenase activity
	biological_process	GO:0006629 - lipid metabolic process GO:0006631 - fatty acid metabolic process GO:0009062 - fatty acid catabolic process GO:0006635 - fatty acid beta-oxidation GO:0055114 - oxidation-reduction process GO:0033539 - fatty acid beta-oxidation using acyl-CoA dehydrogenase

Note(s):

Note(s): ...[more].

Reference(s):

[1] Clomburg JM., et al., 2012
[2] Cronan JE., et al., 1975
[3] Gulevich AY., et al., 2016
[4] Harley JB., et al., 1978
[5] Iram SH., et al., 2006
[6] Lau C., et al., 1983
[7] Lu X., et al., 2003
[8] Olsen WL., et al., 1979

External database links:

ECOCYC:

ACYLCOADEHYDROG-MONOMER

ECOLIWIKI:

INTERPRO:

INTERPRO:

INTERPRO:

INTERPRO:

INTERPRO:

INTERPRO:

INTERPRO:

MODBASE:

PFAM:

PFAM:

PFAM:

PFAM:

PRIDE:

PRODB:

PROSITE:

REFSEQ:

UNIPROT:

Operon

Name:

fadE

Operon arrangement:

Transcription unit	Promoter
fadE	fadEp

Transcriptional Regulation
	Display Regulation
Repressed by:	FadR, ArcA

Elements in the selected gene context region unrelated to any object in RegulonDB

Type	Name	Post Left	Strand	Notes	Evidence (Confirmed, Strong, Weak)	References
promoter	lpcAp1	243468	forward	Similarity to the consensus sequence of the set of known functional promoters for this sigma factor with strong experimental evidence: poor homology. Score: -0.33. P-value: 6.459e-6 Read more >	[ICWHO]	[9]
promoter	TSS_437	243512	forward	nd	[RS-EPT-CBR]	[10]
promoter	TSS_438	244083	forward	nd	[RS-EPT-CBR]	[10]
promoter	TSS_439	244089	forward	nd	[RS-EPT-CBR]	[10]
promoter	TSS_440 (cluster)	244094	forward	For this promoter, there is a cluster of 2 transcription start sites,in the interval 244094 to 244095, where the highest frequency is at position 244095. The most upstream position of this cluster is assigned as TSS position in the promoter. Read more >	[RS-EPT-CBR]	[10]
promoter	TSS_441	244103	forward	nd	[RS-EPT-CBR]	[10]
promoter	TSS_442	244121	forward	nd	[RS-EPT-CBR]	[10]

Evidence
[ICWHO] Inferred computationally without human oversight
[RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates

Reference(s)
[1] Clomburg JM., Vick JE., Blankschien MD., Rodriguez-Moya M., Gonzalez R., 2012, A synthetic biology approach to engineer a functional reversal of the β-oxidation cycle., ACS Synth Biol 1(11):541-54
[2] Cronan JE., Weisberg LJ., Allen RG., 1975, Regulation of membrane lipid synthesis in Escherichia coli. Accumulation of free fatty acids of abnormal length during inhibition of phospholipid synthesis., J Biol Chem 250(15):5835-40
[3] Gulevich AY., Skorokhodova AY., Debabov VG., 2016, [Escherichia coli ydiO and ydiQRST genes encode components of acyl-CoA dehydrogenase complex of anaerobic fatty acid β-oxidation pathway]., Genetika 52(10):1210-4
[4] Harley JB., Santangelo GM., Rasmussen H., Goldfine H., 1978, Dependence of Escherichia coli hyperbaric oxygen toxicity on the lipid acyl chain composition., J Bacteriol 134(3):808-20
[5] Iram SH., Cronan JE., 2006, The beta-oxidation systems of Escherichia coli and Salmonella enterica are not functionally equivalent., J Bacteriol 188(2):599-608
[6] Lau C., Greenway DL., Freter CE., Neungton N., Wattenberg B., Silbert DF., 1983, Conditions influencing formation of 16:0/16:0 molecular species in membrane phospholipids of Escherichia coli., J Biol Chem 258(21):13027-33
[7] Lu X., Zhang J., Wu Q., Chen GQ., 2003, Enhanced production of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) via manipulating the fatty acid beta-oxidation pathway in E. coli., FEMS Microbiol Lett 221(1):97-101
[8] Olsen WL., Schaechter M., Khorana HG., 1979, Incorporation of synthetic fatty acid analogs into phospholipids of Escherichia coli., J Bacteriol 137(3):1443-6
[9] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78
[10] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.