RegulonDB

Gene
Name:	dinJ
Synonym(s):	ECK0227, G6110, b0226, sosA
Genome position(nucleotides):	246242 <-- 246502 Genome Browser
Strand:	reverse
Sequence:	Get nucleotide sequence FastaFormat
GC content %:	46.74
External database links:

ASAP:	ABE-0000764
ECHOBASE:	EB2936
ECOLIHUB:	dinJ
OU-MICROARRAY:	b0226
STRING:	511145.b0226
COLOMBOS:	dinJ

Product

Name:

antitoxin/DNA-binding transcriptional repressor DinJ

Synonym(s):

DinJ, DinJ antitoxin of YafQ-DinJ toxin-antitoxin system / DNA-binding transcriptional repressor, SosA

Sequence:

Get amino acid sequence Fasta Format

Cellular location:

cytosol

Molecular weight:

9.406

Isoelectric point:

5.08

Motif(s):

Type	Positions	Sequence
	4 -> 83	NAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGIDVHKAKDADDLFDK
	35 -> 35	R
	2 -> 44	AANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVARE
	10 -> 10	R

Classification:

Multifun Terms (GenProtEC)
Multifun Terms (GenProtEC)		5 - cell processes --> 5.6 - protection --> 5.6.3 - cell killing
Gene Ontology Terms (GO)
	cellular_component	GO:0005829 - cytosol
	molecular_function	GO:0003677 - DNA binding GO:0005515 - protein binding GO:0015643 - toxic substance binding GO:0042803 - protein homodimerization activity GO:0000986 - bacterial-type cis-regulatory region sequence-specific DNA binding
	biological_process	GO:0006351 - transcription, DNA-templated GO:0006355 - regulation of transcription, DNA-templated GO:0044010 - single-species biofilm formation GO:2000143 - negative regulation of DNA-templated transcription, initiation

Note(s):

Note(s): ...[more].

External database links:

DIP:

ECOCYC:

ECOLIWIKI:

INTERPRO:

INTERPRO:

INTERPRO:

PANTHER:

PDB:

PFAM:

PRIDE:

REFSEQ:

SMR:

UNIPROT:

Operon

Name:

dinJ-yafQ

Operon arrangement:

Transcription unit	Promoter
dinJ-yafQ	dinJp

Transcriptional Regulation
	Display Regulation
Repressed by:	DinJ-YafQ, LexA

Elements in the selected gene context region unrelated to any object in RegulonDB

Type	Name	Post Left	Strand	Notes	Evidence (Confirmed, Strong, Weak)	References
promoter	yafKp6	245993	reverse	nd	[ICWHO]	[1]
promoter	TSS_444	246205	reverse	nd	[RS-EPT-CBR]	[2]
promoter	TSS_445 (cluster)	246533	reverse	nd	[RS-EPT-CBR]	[2]
promoter	yafLp6	246641	forward	nd	[ICWHO]	[1]

Evidence
[ICWHO] Inferred computationally without human oversight
[RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates

Reference(s)
[1] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78
[2] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

Reference(s)

[1] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78

[2] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.