RegulonDB

Gene
Name:	insH1
Synonym(s):	ECK0261, G6134, b0259, trs5, trs5-1, yi52_1
Genome position(nucleotides):	274101 <-- 275081 Genome Browser
Strand:	reverse
Sequence:	Get nucleotide sequence FastaFormat
GC content %:	55.05
External database links:

ASAP:	ABE-0000888
OU-MICROARRAY:	b0259
PortEco:	insH-1
STRING:	511145.b3505
COLOMBOS:	insH1

Product

Name:

CP4-6 prophage; IS5 transposase and trans-activator

Synonym(s):

InsH1, Trs5-1, Yi52_1

Sequence:

Get amino acid sequence Fasta Format

Cellular location:

cytosol

Molecular weight:

37.851

Isoelectric point:

10.028

Motif(s):

Type	Positions	Sequence
	51 -> 124	GRRPYPLETMLRIHCMQHWYNLSDGAMEDALYEIASMRLFARLSLDSALPDRTTIMNFRHLLEQHQLARQLFKT
	138 -> 312	QGTLVDATIIEAPSSTKNKEQQRDPEMHQTKKGNQWHFGMKAHIGVDAKSGLTHSLVTTAANEHDLNQLGNLLHGEEQFVSADAGYQGAPQREELAEVDVDWLIAERPGKVRTLKQHPRKNKTAINIEYMKASIRARVEHPFRIIKRQFGFVKARYKGLLKNDNQLAMLFTLANL

Classification:

Multifun Terms (GenProtEC)
		8 - extrachromosomal --> 8.1 - prophage genes and phage related functions
		8 - extrachromosomal --> 8.3 - transposon related
Gene Ontology Terms (GO)
	cellular_component	GO:0005829 - cytosol
	molecular_function	GO:0003677 - DNA binding GO:0004803 - transposase activity
	biological_process	GO:0006310 - DNA recombination GO:0006313 - transposition, DNA-mediated GO:0032196 - transposition

Note(s):

Note(s): ...[more].

External database links:

ECOCYC:

ECOLIWIKI:

INTERPRO:

INTERPRO:

PFAM:

PFAM:

PRIDE:

REFSEQ:

SMR:

UNIPROT:

Operon

Name:

insH1

Operon arrangement:

Transcription unit	Promoter
insH-1

Elements in the selected gene context region unrelated to any object in RegulonDB

Type	Name	Post Left	Strand	Notes	Evidence (Confirmed, Strong, Weak)	References
promoter	TSS_466	275108	reverse	nd	[RS-EPT-CBR]	[1]
promoter	mmuPp1	275119	forward	Similarity to the consensus sequence of the set of known functional promoters for this sigma factor with strong experimental evidence: poor homology. Score: -3.26. P-value: 6.190e-5 Read more >	[ICWHO]	[2]
promoter	mmuPp6	275206	forward	Similarity to the consensus sequence of the set of known functional promoters for this sigma factor with strong experimental evidence: poor homology. Score: 3.785. P-value: 3.501e-5 Read more >	[ICWHO]	[2]
promoter	mmuPp7	275285	forward	Similarity to the consensus sequence of the set of known functional promoters for this sigma factor with strong experimental evidence: high homology. Score: 4.136. P-value: 8.440e-5 Read more >	[ICWHO]	[2]
promoter	mmuPp5	275287	forward	Similarity to the consensus sequence of the set of known functional promoters for this sigma factor with strong experimental evidence: poor homology. Score: 3.03. P-value: 8.872e-5 Read more >	[ICWHO]	[2]
promoter	insHp5	275298	reverse	Similarity to the consensus sequence of the set of known functional promoters for this sigma factor with strong experimental evidence: good homology. Score: 3.24. P-value: 8.872e-5 Read more >	[ICWHO]	[2]
promoter	mmuPp2	275315	forward	Similarity to the consensus sequence of the set of known functional promoters for this sigma factor with strong experimental evidence: poor homology. Score: -1.67. P-value: 2.166e-5 Read more >	[ICWHO]	[2]

Evidence
[RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates
[ICWHO] Inferred computationally without human oversight

Reference(s)
[1] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.
[2] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78

Reference(s)

[1] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

[2] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78