RegulonDB RegulonDB 11.1: Gene Form
   

mmuM gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

mmuP afuC insH1 mmuM terminator anti-terminator anti-anti-terminator mmuPp2 mmuPp2 insHp5 insHp5 mmuPp5 mmuPp5 mmuPp7 mmuPp7 mmuPp6 mmuPp6 mmuPp1 mmuPp1 TSS_466 TSS_466

Gene      
Name: mmuM    Texpresso search in the literature
Synonym(s): ECK0263, G6136, b0261, yagD
Genome position(nucleotides): 276715 --> 277647
Strand: forward
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
 60.34
External database links:  
ASAP:
 ABE-0000895
ECHOBASE:
 EB3127
ECOLIHUB:
 mmuM
OU-MICROARRAY:
 b0261
STRING:
 511145.b0261
COLOMBOS: mmuM


Product      
Name: CP4-6 prophage; homocysteine S-methyltransferase
Synonym(s): MmuM, YagD
Sequence: Get amino acid sequence Fasta Format
Cellular location: cytosol
Molecular weight: 33.423
Isoelectric point: 5.334
Motif(s):
 
Type Positions Sequence Comment
1 -> 310 MSQNNPLRALLDKQDILLLDGAMATELEARGCNLADSLWSAKVLVENPELIREVHLDYYRAGAQCAITASYQATPAGFAARGLDEAQSKALIGKSVELARKAREAYLAENPQAGTLLVAGSVGPYGAYLADGSEYRGDYHCSVEAFQAFHRPRVEALLDAGADLLACETLPNFSEIEALAELLTAYPRARAWFSFTLRDSEHLSDGTPLRDVVALLAGYPQVVALGINCIALENTTAALQHLHGLTVLPLVVYPNSGEHYDAVSKTWHHHGEHCAQLADYLPQWQAAGARLIGGCCRTTPADIAALKARS UniProt: Hcy-binding.
17 -> 308 LLLDGAMATELEARGCNLADSLWSAKVLVENPELIREVHLDYYRAGAQCAITASYQATPAGFAARGLDEAQSKALIGKSVELARKAREAYLAENPQAGTLLVAGSVGPYGAYLADGSEYRGDYHCSVEAFQAFHRPRVEALLDAGADLLACETLPNFSEIEALAELLTAYPRARAWFSFTLRDSEHLSDGTPLRDVVALLAGYPQVVALGINCIALENTTAALQHLHGLTVLPLVVYPNSGEHYDAVSKTWHHHGEHCAQLADYLPQWQAAGARLIGGCCRTTPADIAALKA

 
Classification:
Multifun Terms (GenProtEC)  
  1 - metabolism --> 1.5 - biosynthesis of building blocks --> 1.5.1 - amino acids --> 1.5.1.9 - methionine
  8 - extrachromosomal --> 8.1 - prophage genes and phage related functions
Gene Ontology Terms (GO)  
cellular_component GO:0005829 - cytosol
molecular_function GO:0047150 - betaine-homocysteine S-methyltransferase activity
GO:0061627 - S-methylmethionine-homocysteine S-methyltransferase activity
GO:0008168 - methyltransferase activity
GO:0016740 - transferase activity
GO:0046872 - metal ion binding
GO:0008270 - zinc ion binding
GO:0008898 - S-adenosylmethionine-homocysteine S-methyltransferase activity
biological_process GO:0033528 - S-methylmethionine cycle
GO:0006974 - cellular response to DNA damage stimulus
GO:0008652 - cellular amino acid biosynthetic process
GO:0009086 - methionine biosynthetic process
GO:0032259 - methylation
GO:0033477 - S-methylmethionine metabolic process
Note(s): Note(s): ...[more].
Reference(s): [1] Wang H., et al., 2015
External database links:  
ALPHAFOLD:
 Q47690
ECOCYC:
 MMUM-MONOMER
ECOLIWIKI:
 b0261
INTERPRO:
 IPR017226
INTERPRO:
 IPR003726
INTERPRO:
 IPR036589
MODBASE:
 Q47690
PDB:
 5DML
PDB:
 5DMN
PDB:
 5DMM
PFAM:
 PF02574
PRIDE:
 Q47690
PRODB:
 PRO_000023251
PROSITE:
 PS50970
REFSEQ:
 NP_414795
SMR:
 Q47690
UNIPROT:
 Q47690


Operon      
Name: mmuPM         
Operon arrangement:
Transcription unit        Promoter
mmuPM


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter TSS_466 275108 reverse nd [RS-EPT-CBR] [2]
  promoter mmuPp1 275119 forward nd [COMP-AINF] [3]
  promoter mmuPp6 275206 forward nd [COMP-AINF] [3]
  promoter mmuPp7 275285 forward nd [COMP-AINF] [3]
  promoter mmuPp5 275287 forward nd [COMP-AINF] [3]
  promoter insHp5 275298 reverse nd [COMP-AINF] [3]
  promoter mmuPp2 275315 forward nd [COMP-AINF] [3]


Evidence    

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates

 [COMP-AINF] Inferred computationally without human oversight


Reference(s)    

 [1] Wang H., Bao B., Zhang Y., Zhang J., Zhang C., Li A., Lu J., Li P., Ying J., Liu Q., Xu T., Yi H., Li J., Zhou L., Zhou T., Xu Z., Ni L., Bao Q., 2015, Molecular variation and horizontal gene transfer of the homocysteine methyltransferase gene mmuM and its distribution in clinical pathogens., Int J Biol Sci 11(1):11-21

 [2] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

 [3] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78

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