RegulonDB

Gene
Name:	insA2
Synonym(s):	ECK0023, G6139, b0265
Genome position(nucleotides):	279600 <-- 279875 Genome Browser
Strand:	reverse
Sequence:	Get nucleotide sequence FastaFormat
GC content %:	53.26
External database links:

ASAP:	ABE-0000909
ECOLIHUB:	insA-2
OU-MICROARRAY:	b0265
STRING:	511145.b4516
COLOMBOS:	insA2

Product

Name:

IS1 protein InsA

Synonym(s):

InsA2

Sequence:

Get amino acid sequence Fasta Format

Molecular weight:

9.902

Isoelectric point:

10.862

Motif(s):

Type	Positions	Sequence
	43 -> 88	FTYTASQPGTHQKIIDMAMNGVGCRATARIMGVGLNTIFRHLKNSG
	1 -> 36	MASVSISCPSCSATDGVVRNGKSTAGHQRYLCSHCR

Classification:

Multifun Terms (GenProtEC)
Multifun Terms (GenProtEC)		8 - extrachromosomal --> 8.3 - transposon related
Gene Ontology Terms (GO)
Gene Ontology Terms (GO)	biological_process	GO:0006310 - DNA recombination GO:0006313 - transposition, DNA-mediated GO:0032196 - transposition

Note(s):

Note(s): ...[more].

Evidence:

[IMP] Inferred from mutant phenotype

Reference(s):

[1] Escoubas JM., et al., 1994

External database links:

ECOCYC:

ECOLIWIKI:

INTERPRO:

INTERPRO:

PFAM:

PFAM:

PRIDE:

REFSEQ:

UNIPROT:

Operon

Name:

insAB2A2B2-afuBC

Operon arrangement:

Transcription unit	Promoter
insA2B2AB2-afuBC

Elements in the selected gene context region unrelated to any object in RegulonDB

Type	Name	Post Left	Strand	Notes	Evidence (Confirmed, Strong, Weak)	References
promoter	TSS_467 (cluster)	279709	forward	For this promoter, there is a cluster of 2 transcription start sites,in the interval 279709 to 279710, where the highest frequency is at position 278934. The most upstream position of this cluster is assigned as TSS position in the promoter. Read more >	[RS-EPT-CBR]	[2]
promoter	TSS_468	279891	reverse	nd	[RS-EPT-CBR]	[2]
promoter	TSS_469	279893	reverse	nd	[RS-EPT-CBR]	[2]

Evidence
[RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates

Reference(s)
[1] Escoubas JM., Lane D., Chandler M., 1994, Is the IS1 transposase, InsAB', the only IS1-encoded protein required for efficient transposition?, J Bacteriol 176(18):5864-7
[2] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

Reference(s)

[1] Escoubas JM., Lane D., Chandler M., 1994, Is the IS1 transposase, InsAB', the only IS1-encoded protein required for efficient transposition?, J Bacteriol 176(18):5864-7

[2] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.