RegulonDB RegulonDB 11.1: Gene Form
   

rclR gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

rclA rclR ykgE anti-anti-terminator anti-terminator terminator ykgEp10 ykgEp10 ykgEp7 ykgEp7 ykgEp8 ykgEp8 ykgEp9 ykgEp9 rclAp rclAp ykgDp2 ykgDp2 ykgCp4 ykgCp4 ykgDp3 ykgDp3 ykgCp8 ykgCp8 TSS_478 TSS_478

Gene      
Name: rclR    Texpresso search in the literature
Synonym(s): ECK0304, G6175, b0305, ykgD
Genome position(nucleotides): 320227 --> 321081
Strand: forward
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
 51.23
External database links:  
ASAP:
 ABE-0001054
ECHOBASE:
 EB3351
ECOLIHUB:
 ykgD
OU-MICROARRAY:
 b0305
STRING:
 511145.b0305
COLOMBOS: rclR


Product      
Name: DNA-binding transcriptional activator RclR
Synonym(s): RclR, YkgD
Sequence: Get amino acid sequence Fasta Format
Regulator Family: AraC/XylS
Cellular location: cytosol
Molecular weight: 31.474
Isoelectric point: 7.293
Motif(s):
 
Type Positions Sequence Comment
2 -> 162 DALSRLLMLNAPQGTIDKNCVLGSDWQLPHGAGELSVIRWHALTQGAAKLEMPTGEIFTLRPGNVVLLPQNSAHRLSHVDNESTCIVCGTLRLQHSARYFLTSLPETLFLAPVNHSVEYNWLREAIPFLQQESRSAMPGVDALCSQICATFFTLAVREWIA
21 -> 21 C UniProt: Does not induce expression of target genes in response to HOCl..
42 -> 42 H UniProt: Increases expression of the target genes in the absence of HOCl..
75 -> 75 H UniProt: Increases expression of the target genes in the absence of HOCl. In vitro, increases DNA binding under reducing conditions..
89 -> 89 C UniProt: Does not induce expression of target genes in response to HOCl..

 
Classification:
Multifun Terms (GenProtEC)  
  2 - information transfer --> 2.2 - RNA related --> 2.2.2 - Transcription related
  3 - regulation --> 3.1 - type of regulation --> 3.1.2 - transcriptional level --> 3.1.2.2 - activator
Gene Ontology Terms (GO)  
cellular_component GO:0005829 - cytosol
molecular_function GO:0003677 - DNA binding
GO:0003700 - DNA-binding transcription factor activity
GO:0043565 - sequence-specific DNA binding
GO:0001216 - DNA-binding transcription activator activity
biological_process GO:0006351 - transcription, DNA-templated
GO:0006355 - regulation of transcription, DNA-templated
GO:0045893 - positive regulation of transcription, DNA-templated
GO:0090347 - regulation of cellular organohalogen metabolic process
Note(s): Note(s): ...[more].
Evidence: [COMP-HINF-FN-FROM-SEQ] Human inference of function from sequence
Reference(s): [1] Perez-Rueda E., et al., 2000
[2] Perez-Rueda E., et al., 2004
External database links:  
ALPHAFOLD:
 P77379
ECOCYC:
 G6175-MONOMER
ECOLIWIKI:
 b0305
INTERPRO:
 IPR011051
INTERPRO:
 IPR009057
INTERPRO:
 IPR020449
INTERPRO:
 IPR032783
INTERPRO:
 IPR018060
MODBASE:
 P77379
PFAM:
 PF12833
PFAM:
 PF12852
PRIDE:
 P77379
PRINTS:
 PR00032
PROSITE:
 PS01124
REFSEQ:
 NP_414839
SMART:
 SM00342
UNIPROT:
 P77379


Operon      
Name: rclR         
Operon arrangement:
Transcription unit        Promoter
rclR


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter TSS_478 320033 reverse nd [RS-EPT-CBR] [3]
  promoter ykgCp8 320088 reverse nd [COMP-AINF] [4]
  promoter ykgDp3 320116 forward nd [COMP-AINF] [4]
  promoter ykgCp4 320170 reverse nd [COMP-AINF] [4]
  promoter ykgDp2 320193 forward nd [COMP-AINF] [4]
  promoter ykgEp9 321481 forward nd [COMP-AINF] [4]
  promoter ykgEp8 321484 forward nd [COMP-AINF] [4]
  promoter ykgEp7 321511 forward nd [COMP-AINF] [4]
  promoter ykgEp10 321537 forward nd [COMP-AINF] [4]


Evidence    

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates

 [COMP-AINF] Inferred computationally without human oversight


Reference(s)    

 [1] Perez-Rueda E., Collado-Vides J., 2000, The repertoire of DNA-binding transcriptional regulators in Escherichia coli K-12., Nucleic Acids Res 28(8):1838-47

 [2] Perez-Rueda E., Collado-Vides J., Segovia L., 2004, Phylogenetic distribution of DNA-binding transcription factors in bacteria and archaea., Comput Biol Chem 28(5-6):341-50

 [3] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

 [4] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78

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