RegulonDB RegulonDB 11.1: Gene Form
   

panE gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

thiI panE yajL yajQ terminator anti-terminator yajQp yajQp TSS_544 TSS_544 TSS_543 TSS_543 TSS_542 (cluster) TSS_542 (cluster) TSS_541 (cluster) TSS_541 (cluster) TSS_540 TSS_540 yajLp2 yajLp2

Gene      
Name: panE    Texpresso search in the literature
Synonym(s): ECK0419, G6239, apbA, b0425
Genome position(nucleotides): 443604 <-- 444515
Strand: reverse
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
 51.86
External database links:  
ASAP:
 ABE-0001474
ECHOBASE:
 EB3056
ECOLIHUB:
 panE
OU-MICROARRAY:
 b0425
STRING:
 511145.b0425
COLOMBOS: panE


Product      
Name: 2-dehydropantoate 2-reductase
Synonym(s): ApbA, PanE
Sequence: Get amino acid sequence Fasta Format
Cellular location: cytosol
Molecular weight: 33.871
Isoelectric point: 5.906
Motif(s):
 
Type Positions Sequence Comment
3 -> 143 ITVLGCGALGQLWLTALCKQGHEVQGWLRVPQPYCSVNLVETDGSIFNESLTANDPDFLATSDLLLVTLKAWQVSDAVKSLASTLPVTTPILLIHNGMGTIEELQNIQQPLLMGTTTHAARRDGNVIIHVANGITHIGPAR
7 -> 12 GCGALG UniProt: NADP.
31 -> 31 R UniProt: Increases KM for NADP 4-fold..
72 -> 72 K UniProt: Increases KM for NADP 10-fold..
98 -> 98 N UniProt: Increases KM for ketopantoate 140-fold. Strongly decreases catalytic activity..

 
Classification:
Multifun Terms (GenProtEC)  
  1 - metabolism --> 1.5 - biosynthesis of building blocks --> 1.5.3 - cofactors, small molecule carriers --> 1.5.3.5 - Coenzyme A and its modification
Gene Ontology Terms (GO)  
cellular_component GO:0005737 - cytoplasm
GO:0005829 - cytosol
molecular_function GO:0016491 - oxidoreductase activity
GO:0008677 - 2-dehydropantoate 2-reductase activity
GO:0050661 - NADP binding
biological_process GO:0015940 - pantothenate biosynthetic process
Note(s): Note(s): ...[more].
Reference(s): [1] Headey SJ., et al., 2008
External database links:  
ALPHAFOLD:
 P0A9J4
ECOCYC:
 2-DEHYDROPANTOATE-REDUCT-MONOMER
ECOLIWIKI:
 B0425
INTERPRO:
 IPR013752
INTERPRO:
 IPR036291
INTERPRO:
 IPR013332
INTERPRO:
 IPR013328
INTERPRO:
 IPR008927
INTERPRO:
 IPR003710
MODBASE:
 P0A9J4
PDB:
 1YJQ
PDB:
 1KS9
PDB:
 1YON
PDB:
 2OFP
PFAM:
 PF08546
PFAM:
 PF02558
PRIDE:
 P0A9J4
PRODB:
 PRO_000023491
REFSEQ:
 NP_414959
SMR:
 P0A9J4
UNIPROT:
 P0A9J4


Operon      
Name: panE-yajL         
Operon arrangement:
Transcription unit        Promoter
panE-yajL


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter yajLp2 443748 reverse nd [COMP-AINF] [2]
  promoter TSS_540 443792 reverse nd [RS-EPT-CBR] [3]
  promoter TSS_541 (cluster) 444555 reverse nd [RS-EPT-CBR] [3]
  promoter TSS_542 (cluster) 444561 reverse nd [RS-EPT-CBR] [3]
  promoter TSS_543 444600 forward nd [RS-EPT-CBR] [3]
  promoter TSS_544 444603 forward nd [RS-EPT-CBR] [3]


Evidence    

 [COMP-AINF] Inferred computationally without human oversight

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates


Reference(s)    

 [1] Headey SJ., Vom A., Simpson JS., Scanlon MJ., 2008, Backbone assignments of the 34 kDa ketopantoate reductase from E. coli., Biomol NMR Assign 2(1):93-6

 [2] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78

 [3] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

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