RegulonDB RegulonDB 10.8: Gene Form
   

yajR gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

yajR cyoE yajQ anti-anti-terminator anti-terminator terminator TSS_546 TSS_546 yajRp yajRp

Gene      
Name: yajR    Texpresso search in the literature
Synonym(s): ECK0421, G6241, b0427
Genome position(nucleotides): 445302 <-- 446666 Genome Browser
Strand: reverse
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
 52.6
External database links:  
ASAP:
 ABE-0001481
ECHOBASE:
 EB3380
OU-MICROARRAY:
 b0427
PortEco:
 yajR
STRING:
 511145.b0427
COLOMBOS: yajR


Product      
Name: putative transport protein YajR
Synonym(s): YajR
Sequence: Get amino acid sequence Fasta Format
Cellular location: inner membrane
Molecular weight: 48.81
Isoelectric point: 9.428
Motif(s):
 
Type Positions Sequence
20 -> 354 VFSLRMLGMFMVLPVLTTYGMALQGASEALIGIAIGIYGLTQAVFQIPFGLLSDRIGRKPLIVGGLAVFAAGSVIAALSDSIWGIILGRALQGSGAIAAAVMALLSDLTREQNRTKAMAFIGVSFGITFAIAMVLGPIITHKLGLHALFWMIAILATTGIALTIWVVPNSSTHVLNRESGMVKGSFSKVLAEPRLLKLNFGIMCLHILLMSTFVALPGQLADAGFPAAEHWKVYLATMLIAFGSVVPFIIYAEVKRKMKQVFVFCVGLIVVAEIVLWNAQTQFWQLVVGVQLFFVAFNLMEALLPSLISKESPAGYKGTAMGVYSTSQFLGVAIG
307 -> 327 VGVQLFFVAFNLMEALLPSLI
364 -> 384 MFDGQGVFLAGAMLAAVWLTV
48 -> 68 ALIGIAIGIYGLTQAVFQIPF
78 -> 97 KPLIVGGLAVFAAGSVIAAL

 
Classification:
Multifun Terms (GenProtEC)  
  4 - transport --> 4.2 - Electrochemical potential driven transporters --> 4.2.A - Porters (Uni-, Sym- and Antiporters)
  5 - cell processes --> 5.6 - protection --> 5.6.4 - drug resistance/sensitivity
  6 - cell structure --> 6.1 - membrane
Gene Ontology Terms (GO)  
cellular_component GO:0016020 - membrane
GO:0005886 - plasma membrane
GO:0005887 - integral component of plasma membrane
GO:0016021 - integral component of membrane
molecular_function GO:0022857 - transmembrane transporter activity
biological_process GO:0055085 - transmembrane transport
Note(s): Note(s): ...[more].
Reference(s): [1] Jiang D., et al., 2013
[2] Jindal S., et al., 2019
External database links:  
ECOCYC:
 B0427-MONOMER
ECOLIWIKI:
 b0427
INTERPRO:
 IPR011701
INTERPRO:
 IPR036259
INTERPRO:
 IPR020846
MODBASE:
 P77726
PDB:
 3WDO
PFAM:
 PF07690
PRIDE:
 P77726
PROSITE:
 PS50850
REFSEQ:
 NP_414961
SMR:
 P77726
UNIPROT:
 P77726


Operon      
Name: yajR         
Operon arrangement:
Transcription unit        Promoter
yajR


RNA cis-regulatory element    
Attenuation: Transcriptional


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter TSS_546 447668 reverse nd [RS-EPT-CBR] [3]


Evidence    

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates


Reference(s)    

 [1] Jiang D., Zhao Y., Wang X., Fan J., Heng J., Liu X., Feng W., Kang X., Huang B., Liu J., Zhang XC., 2013, Structure of the YajR transporter suggests a transport mechanism based on the conserved motif A., Proc Natl Acad Sci U S A 110(36):14664-9

 [2] Jindal S., Yang L., Day PJ., Kell DB., 2019, Involvement of multiple influx and efflux transporters in the accumulation of cationic fluorescent dyes by Escherichia coli., BMC Microbiol 19(1):195

 [3] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

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