RegulonDB RegulonDB 10.10: Gene Form
   

cueR gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

ybaT ybbJ cueR qmcA cueRp6 cueRp6 TSS_658 (cluster) TSS_658 (cluster) TSS_657 TSS_657 TSS_656 TSS_656 TSS_655 TSS_655

Gene      
Name: cueR    Texpresso search in the literature
Synonym(s): ECK0481, G6263, b0487, copR, ybbI
Genome position(nucleotides): 513993 --> 514400 Genome Browser
Strand: forward
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
 58.58
Reference(s): [1] Outten FW., et al., 2000
External database links:  
ASAP:
 ABE-0001693
ECHOBASE:
 EB3044
ECOLIHUB:
 cueR
OU-MICROARRAY:
 b0487
STRING:
 511145.b0487
COLOMBOS: cueR


Product      
Name: DNA-binding transcriptional dual regulator CueR
Synonym(s): CopR, CueR, YbbI
Sequence: Get amino acid sequence Fasta Format
Cellular location: cytosol
Molecular weight: 15.235
Isoelectric point: 6.008
Motif(s):
 
Type Positions Sequence
120 -> 120 C
130 -> 130 C
129 -> 129 C
4 -> 23 SDVAKITGLTSKAIRFYEEK
112 -> 112 C

 
Classification:
Multifun Terms (GenProtEC)  
  2 - information transfer --> 2.2 - RNA related --> 2.2.2 - Transcription related
  3 - regulation --> 3.1 - type of regulation --> 3.1.2 - transcriptional level --> 3.1.2.2 - activator
  3 - regulation --> 3.1 - type of regulation --> 3.1.2 - transcriptional level --> 3.1.2.3 - repressor
Gene Ontology Terms (GO)  
cellular_component GO:0005737 - cytoplasm
GO:0005829 - cytosol
GO:0032993 - protein-DNA complex
molecular_function GO:0003677 - DNA binding
GO:0005507 - copper ion binding
GO:0046872 - metal ion binding
GO:0003700 - DNA-binding transcription factor activity
GO:0042802 - identical protein binding
GO:0000976 - transcription cis-regulatory region binding
GO:0000986 - bacterial-type cis-regulatory region sequence-specific DNA binding
GO:0001216 - DNA-binding transcription activator activity
biological_process GO:0006351 - transcription, DNA-templated
GO:0006355 - regulation of transcription, DNA-templated
GO:0045893 - positive regulation of transcription, DNA-templated
Evidence: [APPH] Assay of protein purified to homogeneity
[GEA] Gene expression analysis
[IFC] Inferred by functional complementation
Reference(s): [1] Outten FW., et al., 2000
[2] Petersen C., et al., 2000
[3] Stoyanov JV., et al., 2003
[4] Stoyanov JV., et al., 2001
[5] Yamamoto K., et al., 2005
External database links:  
DIP:
 DIP-51206N
ECOCYC:
 G6263-MONOMER
ECOLIWIKI:
 b0487
INTERPRO:
 IPR011789
INTERPRO:
 IPR009061
INTERPRO:
 IPR000551
MODBASE:
 P0A9G4
PDB:
 4WLW
PDB:
 1Q05
PDB:
 4WLS
PDB:
 1Q06
PDB:
 1Q07
PFAM:
 PF13411
PRIDE:
 P0A9G4
PRINTS:
 PR00040
PRODB:
 PRO_000022348
PROSITE:
 PS50937
PROSITE:
 PS00552
REFSEQ:
 NP_415020
SMART:
 SM00422
SMR:
 P0A9G4
UNIPROT:
 P0A9G4


Operon      
Name: cueR         
Operon arrangement:
Transcription unit        Promoter
cueR


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter TSS_655 513563 forward nd [RS-EPT-CBR] [6]
  promoter TSS_656 513566 forward nd [RS-EPT-CBR] [6]
  promoter TSS_657 513642 forward nd [RS-EPT-CBR] [6]
  promoter TSS_658 (cluster) 513647 forward nd [RS-EPT-CBR] [6]
  promoter cueRp6 513974 forward nd [ICWHO] [7]


Evidence    

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates

 [ICWHO] Inferred computationally without human oversight


Reference(s)    

 [1] Outten FW., Outten CE., Hale J., O'Halloran TV., 2000, Transcriptional activation of an Escherichia coli copper efflux regulon by the chromosomal MerR homologue, cueR., J Biol Chem 275(40):31024-9

 [2] Petersen C., Moller LB., 2000, Control of copper homeostasis in Escherichia coli by a P-type ATPase, CopA, and a MerR-like transcriptional activator, CopR., Gene 261(2):289-98

 [3] Stoyanov JV., Brown NL., 2003, The Escherichia coli copper-responsive copA promoter is activated by gold., J Biol Chem 278(3):1407-10

 [4] Stoyanov JV., Hobman JL., Brown NL., 2001, CueR (YbbI) of Escherichia coli is a MerR family regulator controlling expression of the copper exporter CopA., Mol Microbiol 39(2):502-11

 [5] Yamamoto K., Ishihama A., 2005, Transcriptional response of Escherichia coli to external copper., Mol Microbiol 56(1):215-27

 [6] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

 [7] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78

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