RegulonDB RegulonDB 10.9: Gene Form
   

insH2 gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

insH2 ybcQ essD MprA IHF CRP OmpR CRP terminator essDp essDp nmpCp nmpCp TSS_685 TSS_685 insHp4 insHp4 TSS_684 TSS_684

Gene      
Name: insH2    Texpresso search in the literature
Synonym(s): ECK0261, G6308, b0552, trs5-2, yi52_2
Genome position(nucleotides): 574737 <-- 575717 Genome Browser
Strand: reverse
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
 55.05
External database links:  
ASAP:
 ABE-0001886
ECOLIHUB:
 insH-2
OU-MICROARRAY:
 b0552
STRING:
 511145.b3505
COLOMBOS: insH2


Product      
Name: DLP12 prophage; IS5 transposase and trans-activator
Synonym(s): InsH2
Sequence: Get amino acid sequence Fasta Format
Cellular location: cytosol
Molecular weight: 37.851
Isoelectric point: 10.028
Motif(s):
 
Type Positions Sequence
51 -> 124 GRRPYPLETMLRIHCMQHWYNLSDGAMEDALYEIASMRLFARLSLDSALPDRTTIMNFRHLLEQHQLARQLFKT
138 -> 312 QGTLVDATIIEAPSSTKNKEQQRDPEMHQTKKGNQWHFGMKAHIGVDAKSGLTHSLVTTAANEHDLNQLGNLLHGEEQFVSADAGYQGAPQREELAEVDVDWLIAERPGKVRTLKQHPRKNKTAINIEYMKASIRARVEHPFRIIKRQFGFVKARYKGLLKNDNQLAMLFTLANL

 
Classification:
Multifun Terms (GenProtEC)  
  8 - extrachromosomal --> 8.1 - prophage genes and phage related functions
  8 - extrachromosomal --> 8.3 - transposon related
Gene Ontology Terms (GO)  
cellular_component GO:0005829 - cytosol
molecular_function GO:0003677 - DNA binding
GO:0004803 - transposase activity
biological_process GO:0006310 - DNA recombination
GO:0006313 - transposition, DNA-mediated
GO:0032196 - transposition
Note(s): Note(s): ...[more].
External database links:  
ECOCYC:
 MONOMER0-4234
ECOLIWIKI:
 b0552
INTERPRO:
 IPR002559
INTERPRO:
 IPR008490
PFAM:
 PF05598
PFAM:
 PF01609
PRIDE:
 P0CE50
REFSEQ:
 NP_415084
UNIPROT:
 P0CE50


Operon      
Name: insH2         
Operon arrangement:
Transcription unit        Promoter
insH-2


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter TSS_684 575744 reverse nd [RS-EPT-CBR] [1]
  promoter insHp4 575774 reverse Similarity to the consensus
Read more > 		[ICWHO] [2]
  promoter TSS_685 576858 forward nd [RS-EPT-CBR] [1]


Evidence    

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates

 [ICWHO] Inferred computationally without human oversight


Reference(s)    

 [1] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

 [2] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78

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