RegulonDB RegulonDB 10.9: Gene Form
   

hcp gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

hcp hcr lysO NarP NarL NarP NarL FNR NsrR hcpp2 hcpp2 hcpp hcpp TSS_1024 TSS_1024 hcrp3 hcrp3

Gene      
Name: hcp    Texpresso search in the literature
Synonym(s): ECK0864, G6457, b0873, priS, ybjW
Genome position(nucleotides): 912162 <-- 913814 Genome Browser
Strand: reverse
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
54.75
External database links:  
ASAP:
ABE-0002962
ECHOBASE:
EB3456
ECOLIHUB:
hcp
OU-MICROARRAY:
b0873
STRING:
511145.b0873
COLOMBOS: hcp


Product      
Name: protein S-nitrosylase
Synonym(s): Hcp, PriS, YbjW, fuscoredoxin, hybrid-cluster protein, nitric oxide reductase, prismane protein
Sequence: Get amino acid sequence Fasta Format
Cellular location: cytosol
Molecular weight: 60.064
Isoelectric point: 5.016
Motif(s):
 
Type Positions Sequence
134 -> 134 E
1 -> 545 MFCVQCEQTIRTPAGNGCSYAQGMCGKTAETSDLQDLLIAALQGLSAWAVKAREYGIINHDVDSFAPRAFFSTLTNVNFDSPRIVGYAREAIALREALKAQCLAVDANARVDNPMADLQLVSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLKGAAAYMEHAHVLGQYDNDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSILDAGETGKYGHPTPTQVNVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLPAHGYPELRKFKHLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVGAYDDRIWTRSIVGWPGVRHLDGDDFSAVITQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVSREKLRHIFLLGGCDGARGERHYFTDFATSVPDDCLILTLACGKYRFNKLEFGDIEGLPRLVDAGQCNDAYSAIILAVTLAEKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVKNIVTGPTAPGFLTPDLLAVLNEKFGLRSITTVEEDMK

 

Classification:
Multifun Terms (GenProtEC)  
  1 - metabolism --> 1.8 - metabolism of other compounds --> 1.8.3 - nitrogen metabolism
Gene Ontology Terms (GO)  
cellular_component GO:0005737 - cytoplasm
GO:0005829 - cytosol
molecular_function GO:0003824 - catalytic activity
GO:0005515 - protein binding
GO:0046872 - metal ion binding
GO:0016491 - oxidoreductase activity
GO:0004601 - peroxidase activity
GO:0051536 - iron-sulfur cluster binding
GO:0051537 - 2 iron, 2 sulfur cluster binding
GO:0016661 - oxidoreductase activity, acting on other nitrogenous compounds as donors
GO:0050418 - hydroxylamine reductase activity
biological_process GO:0035606 - peptidyl-cysteine S-trans-nitrosylation
GO:0018119 - peptidyl-cysteine S-nitrosylation
GO:0006807 - nitrogen compound metabolic process
GO:0042542 - response to hydrogen peroxide
GO:0055114 - oxidation-reduction process
GO:0046210 - nitric oxide catabolic process
GO:0098869 - cellular oxidant detoxification
Note(s): Note(s): ...[more].
Evidence: [APPH] Assay of protein purified to homogeneity
Reference(s): [1] Chismon DL., et al., 2010
[2] Rodionov DA., et al., 2005
[3] Seth D., et al., 2018
[4] van den Berg WA., et al., 2000
External database links:  
DIP:
DIP-9870N
ECOCYC:
G6457-MONOMER
ECOLIWIKI:
b0873
INTERPRO:
IPR016099
INTERPRO:
IPR004137
INTERPRO:
IPR010048
INTERPRO:
IPR011254
INTERPRO:
IPR016100
MODBASE:
P75825
PANTHER:
PTHR30109:SF0
PANTHER:
PTHR30109
PFAM:
PF03063
PRIDE:
P75825
PRODB:
PRO_000022865
REFSEQ:
NP_415394
SMR:
P75825
UNIPROT:
P75825


Operon      
Name: hcp-hcr-poxB-ltaE-ybjT         
Operon arrangement:
Transcription unit        Promoter
ltaE-ybjT
poxB
poxB
hcp-hcr
hcp-hcr-poxB
hcp-hcr
poxB-ltaE-ybjT


Transcriptional Regulation      
Display Regulation             
Activated by: FNR, OxyR, NarL, NarP
Repressed by: NsrR


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter hcrp3 912200 reverse Similarity to the consensus
Read more >
[ICWHO] [5]
  promoter TSS_1024 912408 reverse nd [RS-EPT-CBR] [6]


Evidence    

 [ICWHO] Inferred computationally without human oversight

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates



Reference(s)    

 [1] Chismon DL., Browning DF., Farrant GK., Busby SJ., 2010, Unusual organization, complexity and redundancy at the Escherichia coli hcp-hcr operon promoter., Biochem J 430(1):61-8

 [2] Rodionov DA., Dubchak IL., Arkin AP., Alm EJ., Gelfand MS., 2005, Dissimilatory metabolism of nitrogen oxides in bacteria: comparative reconstruction of transcriptional networks., PLoS Comput Biol 1(5):e55

 [3] Seth D., Hess DT., Hausladen A., Wang L., Wang YJ., Stamler JS., 2018, A Multiplex Enzymatic Machinery for Cellular Protein S-nitrosylation., Mol Cell 69(3):451-464.e6

 [4] van den Berg WA., Hagen WR., van Dongen WM., 2000, The hybrid-cluster protein ('prismane protein') from Escherichia coli. Characterization of the hybrid-cluster protein, redox properties of the [2Fe-2S] and [4Fe-2S-2O] clusters and identification of an associated NADH oxidoreductase containing FAD and [2Fe-2S]., Eur J Biochem 267(3):666-76

 [5] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78

 [6] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.


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