RegulonDB RegulonDB 11.0: Gene Form
   

ycaM gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

ycaM ycaD ycaN terminator anti-terminator anti-anti-terminator ycaKp3 ycaKp3 ycaMp3 ycaMp3 ycaMp5 ycaMp5 ycaMp4 ycaMp4 ycaMp2 ycaMp2

Gene      
Name: ycaM    Texpresso search in the literature
Synonym(s): ECK0890, G6466, b0899
Genome position(nucleotides): 947229 --> 948659
Strand: forward
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
 47.17
External database links:  
ASAP:
 ABE-0003058
ECHOBASE:
 EB3461
ECOLIHUB:
 ycaM
OU-MICROARRAY:
 b0899
STRING:
 511145.b0899
COLOMBOS: ycaM


Product      
Name: putative transporter YcaM
Synonym(s): YcaM
Sequence: Get amino acid sequence Fasta Format
Cellular location: inner membrane
Molecular weight: 52.496
Isoelectric point: 9.502
Motif(s):
 
Type Positions Sequence Comment
10 -> 30 LRWYNIALMSFITVWGFGNVV UniProt: Helical.
11 -> 448 RWYNIALMSFITVWGFGNVVNNYANQGLVVVFSWVFIFALYFTPYALIVGQLGSTFKDGKGGVSTWIKHTMGPGLAYLAAWTYWVVHIPYLAQKPQAILIALGWAMKGDGSLIKEYSVVALQGLTLVLFIFFMWVASRGMKSLKIVGSVAGIAMFVMSLLYVAMAVTAPAITEVHIATTNITWETFIPHIDFTYITTISMLVFAVGGAEKISPYVNQTRNPGKEFPKGMLCLAVMVAVCAILGSLAMGMMFDSRNIPDDLMTNGQYYAFQKLGEYYNMGNTLMVIYAIANTLGQVAALVFSIDAPLKVLLGDADSKYIPASLCRTNASGTPVNGYFLTLVLVAILIMLPTLGIGDMNNLYKWLLNLNSVVMPLRYLWVFVAFIAVVRLAQKYKPEYVFIRNKPLAMTVGIWCFAFTAFACLTGIFPKMEAFTAEWTFQ
39 -> 59 VVVFSWVFIFALYFTPYALIV UniProt: Helical.
81 -> 101 MGPGLAYLAAWTYWVVHIPYL UniProt: Helical.
126 -> 146 YSVVALQGLTLVLFIFFMWVA UniProt: Helical.

 
Classification:
Multifun Terms (GenProtEC)  
  4 - transport --> 4.2 - Electrochemical potential driven transporters --> 4.2.A - Porters (Uni-, Sym- and Antiporters)
  6 - cell structure --> 6.1 - membrane
Gene Ontology Terms (GO)  
cellular_component GO:0016020 - membrane
GO:0005886 - plasma membrane
GO:0016021 - integral component of membrane
molecular_function GO:0015171 - amino acid transmembrane transporter activity
GO:0022857 - transmembrane transporter activity
biological_process GO:0006865 - amino acid transport
GO:0055085 - transmembrane transport
GO:0003333 - amino acid transmembrane transport
Note(s): Note(s): ...[more].
Evidence: [IMP] Inferred from mutant phenotype
Reference(s): [1] Jindal S., et al., 2019
[2] Serina S., et al., 2004
External database links:  
ALPHAFOLD:
 P75835
DIP:
 DIP-11468N
ECOCYC:
 YCAM
ECOLIWIKI:
 b0899
INTERPRO:
 IPR002293
PFAM:
 PF13520
PRIDE:
 P75835
REFSEQ:
 NP_415419
UNIPROT:
 P75835


Operon      
Name: ycaM         
Operon arrangement:
Transcription unit        Promoter
ycaM


RNA cis-regulatory element    
Attenuation: Transcriptional


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter ycaMp2 947120 forward nd [ICWHO] [3]
  promoter ycaMp4 947127 forward nd [ICWHO] [3]
  promoter ycaMp5 947144 forward nd [ICWHO] [3]
  promoter ycaMp3 947145 forward nd [ICWHO] [3]
  promoter ycaKp3 949547 forward nd [ICWHO] [3]


Evidence    

 [ICWHO] Inferred computationally without human oversight


Reference(s)    

 [1] Jindal S., Yang L., Day PJ., Kell DB., 2019, Involvement of multiple influx and efflux transporters in the accumulation of cationic fluorescent dyes by Escherichia coli., BMC Microbiol 19(1):195

 [2] Serina S., Nozza F., Nicastro G., Faggioni F., Mottl H., Deho G., Polissi A., 2004, Scanning the Escherichia coli chromosome by random transposon mutagenesis and multiple phenotypic screening., Res Microbiol 155(8):692-701

 [3] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78

RegulonDB