RegulonDB

Gene
Name:	insB4
Synonym(s):	ECK0022, G6509, b0988
Genome position(nucleotides):	1050027 --> 1050530 Genome Browser
Strand:	forward
Sequence:	Get nucleotide sequence FastaFormat
GC content %:	54.76
External database links:

ASAP:	ABE-0003338
ECHOBASE:	EB4719
ECOLIHUB:	insB-4
ECOO157CYC:	Z1123
ECOO157CYC:	Z5491
ECOO157CYC:	Z1632
ECOO157CYC:	Z1192
ECOO157CYC:	Z1562
OU-MICROARRAY:	b0988
STRING:	511145.b0988
COLOMBOS:	insB4

Product

Name:

IS1 protein InsB

Synonym(s):

InsB4

Sequence:

Get amino acid sequence Fasta Format

Cellular location:

cytosol

Molecular weight:

19.7

Isoelectric point:

10.672

Motif(s):

Type	Positions	Sequence
	37 -> 167	VIVCAEMDEQWGYVGAKSRQRWLFYAYDRLRKTVVAHVFGERTMATLGRLMSLLSPFDVVIWMTDGWPLYESRLKGKLHVISKRYTQRIERYNLNLRQHLARLGRKSLSFSKSVELHDKVIGHYLNIKHYQ

Classification:

Multifun Terms (GenProtEC)
Multifun Terms (GenProtEC)		8 - extrachromosomal --> 8.3 - transposon related
Gene Ontology Terms (GO)
	cellular_component	GO:0005829 - cytosol
	molecular_function	GO:0003677 - DNA binding GO:0004803 - transposase activity
	biological_process	GO:0006310 - DNA recombination GO:0006313 - transposition, DNA-mediated GO:0032196 - transposition

Note(s):

Note(s): ...[more].

Evidence:

[IMP] Inferred from mutant phenotype

Reference(s):

[1] Escoubas JM., et al., 1994

External database links:

ECOCYC:

ECOLIWIKI:

ECOO157CYC:

ECOO157CYC:

ECOO157CYC:

ECOO157CYC:

ECOO157CYC:

INTERPRO:

PFAM:

PRIDE:

PRODB:

REFSEQ:

UNIPROT:

Operon

Name:

insA4AB4B4

Operon arrangement:

Transcription unit	Promoter
insA4AB4B4

Elements in the selected gene context region unrelated to any object in RegulonDB

Type	Name	Post Left	Strand	Notes	Evidence (Confirmed, Strong, Weak)	References
promoter	gfcAp1	1049834	reverse	Similarity to the consensus sequence of the set of known functional promoters for this sigma factor with strong experimental evidence: poor homology. Score: -3.53. P-value: 9.516e-5 Read more >	[ICWHO]	[2]
promoter	TSS_1308	1049998	reverse	nd	[RS-EPT-CBR]	[3]
promoter	TSS_1309 (cluster)	1050701	reverse	For this promoter, there is a cluster of 2 transcription start sites,in the interval 1050700 to 1050701, where the highest frequency is at position 1049924. The most upstream position of this cluster is assigned as TSS position in the promoter. Read more >	[RS-EPT-CBR]	[3]

Evidence
[ICWHO] Inferred computationally without human oversight
[RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates

Reference(s)
[1] Escoubas JM., Lane D., Chandler M., 1994, Is the IS1 transposase, InsAB', the only IS1-encoded protein required for efficient transposition?, J Bacteriol 176(18):5864-7
[2] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78
[3] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.