RegulonDB

Gene
Name:	torT
Synonym(s):	ECK0985, G6515, b0994, yccH
Genome position(nucleotides):	1056261 --> 1057289
Strand:	forward
Sequence:	Get nucleotide sequence FastaFormat
GC content %:	53.45
External database links:

ASAP:	ABE-0003360
ECHOBASE:	EB2500
ECOLIHUB:	torT
OU-MICROARRAY:	b0994
STRING:	511145.b0994
COLOMBOS:	torT

Product

Name:

periplasmic trimethylamine-N-oxide binding protein

Synonym(s):

TorT, YccH

Sequence:

Get amino acid sequence Fasta Format

Cellular location:

periplasmic space

Molecular weight:

37.865

Isoelectric point:

9.385

Motif(s):

Type	Positions	Sequence	Comment
	1 -> 18	MRVLLFLLLSLFMLPAFS
	19 -> 342	ADNLLRWHDAQHFTVQASTPLKAKRAWKLCALYPSLKDSYWLSLNYGMQEAARRYGVDLKVLEAGGYSQLATQQAQIDQCKQWGAEAILLGSSTTSFPDLQKQVASLPVIELVNAIDAPQVKSRVGVPWFQMGYQPGRYLVQWAHGKPLNVLLMPGPDNAGGSKEMVEGFRAAIAGSPVRIVDIALGDNDIEIQRNLLQEMLERHPEIDVVAGTAIAAEAAMGEGRNLKTPLTVVSFYLSHQVYRGLKRGRVIMAASDQMVWQGELAVEQAIRQLQGQSVSDNVSPPILVLTPKNADREHIRRSLSPGGFRPVYFYQHTSAAKK	UniProt: Periplasmic protein TorT.
	44 -> 337	AWKLCALYPSLKDSYWLSLNYGMQEAARRYGVDLKVLEAGGYSQLATQQAQIDQCKQWGAEAILLGSSTTSFPDLQKQVASLPVIELVNAIDAPQVKSRVGVPWFQMGYQPGRYLVQWAHGKPLNVLLMPGPDNAGGSKEMVEGFRAAIAGSPVRIVDIALGDNDIEIQRNLLQEMLERHPEIDVVAGTAIAAEAAMGEGRNLKTPLTVVSFYLSHQVYRGLKRGRVIMAASDQMVWQGELAVEQAIRQLQGQSVSDNVSPPILVLTPKNADREHIRRSLSPGGFRPVYFYQHT
	59 -> 59	W	UniProt: In Ref. 1; CAA63921..
	80 -> 80	L	L → P: is not resistant to proteoytic digestion in the presence of TMAO suggesting that this mutant is unable to bind TMAO

Classification:

Multifun Terms (GenProtEC)
		1 - metabolism --> 1.3 - energy metabolism, carbon --> 1.3.7 - anaerobic respiration
		2 - information transfer --> 2.3 - protein related --> 2.3.3 - posttranslational modification
		3 - regulation --> 3.1 - type of regulation --> 3.1.2 - transcriptional level --> 3.1.2.4 - complex regulation --> 3.1.2.4.3 - two component regulatory systems (external signal)
Gene Ontology Terms (GO)
	cellular_component	GO:0030288 - outer membrane-bounded periplasmic space GO:0042597 - periplasmic space
	molecular_function	GO:0003700 - DNA-binding transcription factor activity GO:0000976 - transcription cis-regulatory region binding
	biological_process	GO:0006355 - regulation of transcription, DNA-templated GO:0009061 - anaerobic respiration

Note(s):

Note(s): ...[more].

Evidence:

[EXP-IDA-PURIFIED-PROTEIN] Assay of protein purified to homogeneity
[EXP-IMP] Inferred from mutant phenotype

Reference(s):

[1] Baraquet C., et al., 2006
[2] Jourlin C., et al., 1996

External database links:

ALPHAFOLD:

ECOCYC:

ECOLIWIKI:

INTERPRO:

INTERPRO:

INTERPRO:

MODBASE:

PFAM:

PRIDE:

PRODB:

REFSEQ:

SMR:

UNIPROT:

Type	Name	Post Left	Strand	Notes	Evidence (Confirmed, Strong, Weak)	References
promoter	torSp5	1056182	reverse	nd	[COMP-AINF]	[3]
promoter	torTp7	1056207	forward	nd	[COMP-AINF]	[3]
promoter	torTp1	1056220	forward	nd	[COMP-AINF]	[3]
promoter	torTp2	1056222	forward	nd	[COMP-AINF]	[3]

Reference(s)
[1] Baraquet C., Theraulaz L., Guiral M., Lafitte D., Mejean V., Jourlin-Castelli C., 2006, TorT, a member of a new periplasmic binding protein family, triggers induction of the Tor respiratory system upon TMAO electron-acceptor binding in Escherichia coli., J Biol Chem 281(50):38189-99
[2] Jourlin C., Simon G., Pommier J., Chippaux M., Mejean V., 1996, The periplasmic TorT protein is required for trimethylamine N-oxide reductase gene induction in Escherichia coli., J Bacteriol 178(4):1219-23
[3] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78

RNA cis-regulatory element

Attenuation:	Translational