RegulonDB RegulonDB 11.1: Gene Form
   

rutG gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

rutG rutF ymdF ycdF rutGp2 rutGp2

Gene      
Name: rutG    Texpresso search in the literature
Synonym(s): ECK0997, G6517, b1006, ycdG
Genome position(nucleotides): 1068511 <-- 1069839
Strand: reverse
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
 54.03
External database links:  
ASAP:
 ABE-0003401
ECHOBASE:
 EB3613
ECOLIHUB:
 rutG
OU-MICROARRAY:
 b1006
STRING:
 511145.b1006
COLOMBOS: rutG


Product      
Name: pyrimidine:H+ symporter
Synonym(s): RutG, YcdG
Sequence: Get amino acid sequence Fasta Format
Cellular location: inner membrane
Molecular weight: 45.557
Isoelectric point: 8.814
Motif(s):
 
Type Positions Sequence Comment
31 -> 401 QTAVMGVQHAVAMFGATVLMPILMGLDPNLSILMSGIGTLLFFFITGGRVPSYLGSSAAFVGVVIAATGFNGQGINPNISIALGGIIACGLVYTVIGLVVMKIGTRWIERLMPPVVTGAVVMAIGLNLAPIAVKSVSASAFDSWMAVMTVLCIGLVAVFTRGMIQRLLILVGLIVACLLYGVMTNVLGLGKAVDFTLVSHAAWFGLPHFSTPAFNGQAMMLIAPVAVILVAENLGHLKAVAGMTGRNMDPYMGRAFVGDGLATMLSGSVGGSGVTTYAENIGVMAVTKVYSTLVFVAAAVIAMLLGFSPKFGALIHTIPAAVIGGASIVVFGLIAVAGARIWVQNRVDLSQNGNLIMVAVTLVLGAGDFAL
58 -> 78 PNLSILMSGIGTLLFFFITGG UniProt: Helical.
80 -> 100 VPSYLGSSAAFVGVVIAATGF UniProt: Helical.
87 -> 87 S S → V: loss of function mutation; no detectable uracil or thymine uptake activity
88 -> 88 A A → F: compromised for thymine uptake

 
Classification:
Multifun Terms (GenProtEC)  
  4 - transport --> 4.2 - Electrochemical potential driven transporters --> 4.2.A - Porters (Uni-, Sym- and Antiporters)
  6 - cell structure --> 6.1 - membrane
Gene Ontology Terms (GO)  
cellular_component GO:0016020 - membrane
GO:0005886 - plasma membrane
GO:0005887 - integral component of plasma membrane
GO:0016021 - integral component of membrane
molecular_function GO:0005350 - pyrimidine nucleobase transmembrane transporter activity
GO:0015293 - symporter activity
GO:0022857 - transmembrane transporter activity
GO:0015210 - uracil transmembrane transporter activity
GO:0015505 - uracil:cation symporter activity
GO:0015218 - pyrimidine nucleotide transmembrane transporter activity
biological_process GO:0098721 - uracil import across plasma membrane
GO:0055085 - transmembrane transport
GO:0006212 - uracil catabolic process
GO:0006864 - pyrimidine nucleotide transport
GO:1904082 - pyrimidine nucleobase transmembrane transport
Note(s): Note(s): ...[more].
Reference(s): [1] Jindal S., et al., 2019
External database links:  
ALPHAFOLD:
 P75892
ECOCYC:
 B1006-MONOMER
ECOLIWIKI:
 b1006
INTERPRO:
 IPR006043
INTERPRO:
 IPR006042
INTERPRO:
 IPR019918
PFAM:
 PF00860
PRIDE:
 P75892
PROSITE:
 PS01116
REFSEQ:
 NP_415526
SMR:
 P75892
UNIPROT:
 P75892


Operon      
Name: rutABCDEFG         
Operon arrangement:
Transcription unit        Promoter
rutABCDEFG


Transcriptional Regulation      
Display Regulation             
Activated by: PhoP, NtrC, ArcA
Repressed by: RutR


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter rutGp2 1070045 reverse nd [COMP-AINF] [2]


Evidence    

 [COMP-AINF] Inferred computationally without human oversight


Reference(s)    

 [1] Jindal S., Yang L., Day PJ., Kell DB., 2019, Involvement of multiple influx and efflux transporters in the accumulation of cationic fluorescent dyes by Escherichia coli., BMC Microbiol 19(1):195

 [2] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78

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