RegulonDB

Gene
Name:	csgF
Synonym(s):	ECK1024, G6544, b1038
Genome position(nucleotides):	1101711 <-- 1102127 Genome Browser
Strand:	reverse
Sequence:	Get nucleotide sequence FastaFormat
GC content %:	42.45
External database links:

ASAP:	ABE-0003519
ECHOBASE:	EB3188
ECOLIHUB:	csgF
OU-MICROARRAY:	b1038
STRING:	511145.b1038
COLOMBOS:	csgF

Product

Name:

curli assembly component CsgF

Synonym(s):

CsgF

Sequence:

Get amino acid sequence Fasta Format

Cellular location:

outer membrane

Molecular weight:

15.056

Isoelectric point:

5.753

Motif(s):

Type	Positions	Sequence
	1 -> 19	MRVKHAVVLLMLISPLSWA
	26 -> 26	F
	31 -> 31	F
	20 -> 138	GTMTFQFRNPNFGGNPNNGAFLLNSAQAQNSYKDPSYNDDFGIETPSALDNFTQAIQSQILGGLLSNINTGKPGRMVTNDYIVDIANRDGQLQLNVTDRKTGQTSTIQVSGLQNNSTDF
	24 -> 24	F

Classification:

Multifun Terms (GenProtEC)
		1 - metabolism --> 1.6 - biosynthesis of macromolecules (cellular constituents) --> 1.6.13 - fimbri, pili
		6 - cell structure --> 6.1 - membrane
Gene Ontology Terms (GO)
	cellular_component	GO:0062155 - curli secretion complex GO:0009279 - cell outer membrane GO:0016020 - membrane
	molecular_function	GO:0005515 - protein binding GO:0042802 - identical protein binding
	biological_process	GO:0098775 - curli assembly GO:0098777 - protein secretion by the type VIII secretion system

Note(s):

Note(s): ...[more].

Evidence:

[APPH] Assay of protein purified to homogeneity
[IMP] Inferred from mutant phenotype

Reference(s):

[1] Hammar M., et al., 1995
[2] Schubeis T., et al., 2018

External database links:

DISPROT:

ECOCYC:

ECOLIWIKI:

INTERPRO:

PDB:

PFAM:

PRIDE:

REFSEQ:

SMR:

UNIPROT:

Transcriptional Regulation
	Display Regulation
Activated by:	CRP, OmpR, IHF, Cra, RcdA, BasR, BolA, CsgD, MlrA
Repressed by:	MqsA, FliZ, CpxR, H-NS, RcsAB, RstA, BtsR

Elements in the selected gene context region unrelated to any object in RegulonDB

	Type	Name	Post Left	Post Right	Strand	Notes	Evidence (Confirmed, Strong, Weak)	References
	promoter	csgGp1	1101755		reverse	Similarity to the consensus sequence of the set of known functional promoters for this sigma factor with strong experimental evidence: poor homology. Score: -0.66. P-value: 8.895e-6 Read more >	[ICWHO]	[3]

Reference(s)
[1] Hammar M., Arnqvist A., Bian Z., Olsen A., Normark S., 1995, Expression of two csg operons is required for production of fibronectin- and congo red-binding curli polymers in Escherichia coli K-12., Mol Microbiol 18(4):661-70
[2] Schubeis T., Spehr J., Viereck J., Kopping L., Nagaraj M., Ahmed M., Ritter C., 2018, Structural and functional characterization of the Curli adaptor protein CsgF., FEBS Lett 592(6):1020-1029
[3] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78