RegulonDB RegulonDB 11.0: Gene Form
   

csgF gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

csgG csgF csgE csgGp1 csgGp1

Gene      
Name: csgF    Texpresso search in the literature
Synonym(s): ECK1024, G6544, b1038
Genome position(nucleotides): 1101711 <-- 1102127
Strand: reverse
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
 42.45
External database links:  
ASAP:
 ABE-0003519
ECHOBASE:
 EB3188
ECOLIHUB:
 csgF
OU-MICROARRAY:
 b1038
STRING:
 511145.b1038
COLOMBOS: csgF


Product      
Name: curli assembly component CsgF
Synonym(s): CsgF
Sequence: Get amino acid sequence Fasta Format
Cellular location: outer membrane
Molecular weight: 15.056
Isoelectric point: 5.753
Motif(s):
 
Type Positions Sequence Comment
1 -> 19 MRVKHAVVLLMLISPLSWA
6 -> 129 AVVLLMLISPLSWAGTMTFQFRNPNFGGNPNNGAFLLNSAQAQNSYKDPSYNDDFGIETPSALDNFTQAIQSQILGGLLSNINTGKPGRMVTNDYIVDIANRDGQLQLNVTDRKTGQTSTIQVS
20 -> 138 GTMTFQFRNPNFGGNPNNGAFLLNSAQAQNSYKDPSYNDDFGIETPSALDNFTQAIQSQILGGLLSNINTGKPGRMVTNDYIVDIANRDGQLQLNVTDRKTGQTSTIQVSGLQNNSTDF UniProt: Curli production assembly/transport component CsgF.
24 -> 24 F F → D: loss of interaction with CsgF; curli fibers do not bind to cell surface
26 -> 26 F F → D: loss of interaction with CsgF; curli fibers do not bind to cell surface

 
Classification:
Multifun Terms (GenProtEC)  
  1 - metabolism --> 1.6 - biosynthesis of macromolecules (cellular constituents) --> 1.6.13 - fimbri, pili
  6 - cell structure --> 6.1 - membrane
Gene Ontology Terms (GO)  
cellular_component GO:0062155 - curli secretion complex
GO:0009279 - cell outer membrane
GO:0016020 - membrane
molecular_function GO:0005515 - protein binding
GO:0042802 - identical protein binding
biological_process GO:0098775 - curli assembly
GO:0098777 - protein secretion by the type VIII secretion system
Note(s): Note(s): ...[more].
Evidence: [APPH] Assay of protein purified to homogeneity
[IMP] Inferred from mutant phenotype
Reference(s): [1] Hammar M., et al., 1995
[2] Schubeis T., et al., 2018
External database links:  
ALPHAFOLD:
 P0AE98
DISPROT:
 DP01177
ECOCYC:
 G6544-MONOMER
ECOLIWIKI:
 b1038
INTERPRO:
 IPR018893
PDB:
 6LQJ
PDB:
 7BRM
PDB:
 6SI7
PDB:
 6LQH
PDB:
 6L7C
PDB:
 5M1U
PDB:
 6L7A
PFAM:
 PF10614
PRIDE:
 P0AE98
REFSEQ:
 NP_415556
SMR:
 P0AE98
UNIPROT:
 P0AE98


Operon      
Name: csgDEFG         
Operon arrangement:
Transcription unit        Promoter
csgDEFG
csgDEFG
csgDEFG
csgDEFG


Transcriptional Regulation      
Display Regulation             
Activated by: CRP, OmpR, IHF, Cra, RcdA, BasR, BolA, CsgD, MlrA
Repressed by: MqsA, FliZ, H-NS, CpxR, RcsAB, RstA, BtsR


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter csgGp1 1101755 reverse nd [ICWHO] [3]


Evidence    

 [ICWHO] Inferred computationally without human oversight


Reference(s)    

 [1] Hammar M., Arnqvist A., Bian Z., Olsen A., Normark S., 1995, Expression of two csg operons is required for production of fibronectin- and congo red-binding curli polymers in Escherichia coli K-12., Mol Microbiol 18(4):661-70

 [2] Schubeis T., Spehr J., Viereck J., Kopping L., Nagaraj M., Ahmed M., Ritter C., 2018, Structural and functional characterization of the Curli adaptor protein CsgF., FEBS Lett 592(6):1020-1029

 [3] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78

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