RegulonDB

Gene
Name:	csgE
Synonym(s):	ECK1025, G6545, b1039
Genome position(nucleotides):	1102152 <-- 1102541 Genome Browser
Strand:	reverse
Sequence:	Get nucleotide sequence FastaFormat
GC content %:	43.08
External database links:

ASAP:	ABE-0003528
ECHOBASE:	EB3187
ECOLIHUB:	csgE
OU-MICROARRAY:	b1039
STRING:	511145.b1039
COLOMBOS:	csgE

Gene

Name:

csgE

Synonym(s):

ECK1025, G6545, b1039

Genome position(nucleotides):

1102152 <-- 1102541 Genome Browser

Strand:

reverse

Sequence:

Get nucleotide sequence FastaFormat

GC content %:

43.08

External database links:

ASAP:

ECHOBASE:

ECOLIHUB:

OU-MICROARRAY:

STRING:

COLOMBOS:

Product

Name:

curli assembly component CsgE

Synonym(s):

CsgE

Sequence:

Get amino acid sequence Fasta Format

Cellular location:

outer membrane,cytosol

Molecular weight:

14.879

Isoelectric point:

5.156

Motif(s):

Type	Positions	Sequence
	27 -> 128	VPGLLTDHTVSSIGHDFYRAFSDKWESDYTGNLTINERPSARWGSWITITVNQDVIFQTFLFPLKRDFEKTVVFALIQTEEALNRRQINQALLSTGDLAHDE
	52 -> 52	E
	1 -> 22	MKRYLRWIVAAEFLFAAGNLHA
	72 -> 72	W
	23 -> 129	VEVEVPGLLTDHTVSSIGHDFYRAFSDKWESDYTGNLTINERPSARWGSWITITVNQDVIFQTFLFPLKRDFEKTVVFALIQTEEALNRRQINQALLSTGDLAHDEF

Classification:

Multifun Terms (GenProtEC)
Multifun Terms (GenProtEC)		4 - transport --> 4.8.A - Accessory Factors Involved in Transport
Gene Ontology Terms (GO)
	cellular_component	GO:0062155 - curli secretion complex GO:0005829 - cytosol GO:0009279 - cell outer membrane
	molecular_function	GO:0005515 - protein binding GO:0042802 - identical protein binding
	biological_process	GO:0098775 - curli assembly GO:0098777 - protein secretion by the type VIII secretion system

Note(s):

Note(s): ...[more].

Evidence:

[IDA] Inferred from direct assay
[IMP] Inferred from mutant phenotype

Reference(s):

[1] Goyal P., et al., 2014
[2] Hammar M., et al., 1995
[3] Wang H., et al., 2015

External database links:

DIP:

ECOCYC:

ECOLIWIKI:

INTERPRO:

PDB:

PFAM:

PRIDE:

REFSEQ:

SMR:

UNIPROT:

Transcriptional Regulation
	Display Regulation
Activated by:	CRP, OmpR, IHF, RcdA, Cra, BasR, BolA, CsgD, MlrA
Repressed by:	FliZ, MqsA, H-NS, CpxR, RcsAB, RstA, BtsR

Transcriptional Regulation

Display Regulation

Activated by:

CRP, OmpR, IHF, RcdA, Cra, BasR, BolA, CsgD, MlrA

Repressed by:

FliZ, MqsA, H-NS, CpxR, RcsAB, RstA, BtsR

Elements in the selected gene context region unrelated to any object in RegulonDB

	Type	Name	Post Left	Post Right	Strand	Notes	Evidence (Confirmed, Strong, Weak)	References
	promoter	csgGp1	1101755		reverse	Similarity to the consensus sequence of the set of known functional promoters for this sigma factor with strong experimental evidence: poor homology. Score: -0.66. P-value: 8.895e-6 Read more >	[ICWHO]	[4]

Evidence
[ICWHO] Inferred computationally without human oversight

Evidence

[ICWHO] Inferred computationally without human oversight

Reference(s)
[1] Goyal P., Krasteva PV., Van Gerven N., Gubellini F., Van den Broeck I., Troupiotis-Tsailaki A., Jonckheere W., Pehau-Arnaudet G., Pinkner JS., Chapman MR., Hultgren SJ., Howorka S., Fronzes R., Remaut H., 2014, Structural and mechanistic insights into the bacterial amyloid secretion channel CsgG., Nature 516(7530):250-3
[2] Hammar M., Arnqvist A., Bian Z., Olsen A., Normark S., 1995, Expression of two csg operons is required for production of fibronectin- and congo red-binding curli polymers in Escherichia coli K-12., Mol Microbiol 18(4):661-70
[3] Wang H., Shu Q., Rempel DL., Frieden C., Gross ML., 2015, Continuous and pulsed hydrogen-deuterium exchange and mass spectrometry characterize CsgE oligomerization., Biochemistry 54(42):6475-81
[4] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78

Reference(s)

[1] Goyal P., Krasteva PV., Van Gerven N., Gubellini F., Van den Broeck I., Troupiotis-Tsailaki A., Jonckheere W., Pehau-Arnaudet G., Pinkner JS., Chapman MR., Hultgren SJ., Howorka S., Fronzes R., Remaut H., 2014, Structural and mechanistic insights into the bacterial amyloid secretion channel CsgG., Nature 516(7530):250-3

[2] Hammar M., Arnqvist A., Bian Z., Olsen A., Normark S., 1995, Expression of two csg operons is required for production of fibronectin- and congo red-binding curli polymers in Escherichia coli K-12., Mol Microbiol 18(4):661-70

[3] Wang H., Shu Q., Rempel DL., Frieden C., Gross ML., 2015, Continuous and pulsed hydrogen-deuterium exchange and mass spectrometry characterize CsgE oligomerization., Biochemistry 54(42):6475-81

[4] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78