RegulonDB RegulonDB 10.8: Gene Form
   

thiK gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

nagZ thiK lpoB ycfL TSS_1438 TSS_1438 TSS_1437 TSS_1437 TSS_1436 TSS_1436

Gene      
Name: thiK    Texpresso search in the literature
Synonym(s): ECK1092, G6566, b1106, ycfN
Genome position(nucleotides): 1163260 --> 1164084 Genome Browser
Strand: forward
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
53.33
External database links:  
ASAP:
ABE-0003735
ECHOBASE:
EB3206
OU-MICROARRAY:
b1106
PortEco:
thiK
STRING:
511145.b1106
COLOMBOS: thiK


Product      
Name: thiamine kinase
Synonym(s): ThiK, YcfN
Sequence: Get amino acid sequence Fasta Format
Cellular location: cytosol
Molecular weight: 32.397
Isoelectric point: 10.16
Motif(s):
 
Type Positions Sequence
25 -> 222 VTTFNSGLSGGSFLIEHQGQRFVVRQPHDPDAPQSAFLRQYRALSQLPACIAPKPHLYLRDWMVVDYLPGAVKTYLPDTNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRMLKRLRKAREPRPLRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDIALELAAVWVENTEQHRQLVNDYATRAK

 

Classification:
Multifun Terms (GenProtEC)  
  1 - metabolism --> 1.5 - biosynthesis of building blocks --> 1.5.3 - cofactors, small molecule carriers --> 1.5.3.8 - thiamin
Gene Ontology Terms (GO)  
cellular_component GO:0005829 - cytosol
molecular_function GO:0016740 - transferase activity
GO:0016301 - kinase activity
GO:0000166 - nucleotide binding
GO:0005524 - ATP binding
GO:0019165 - thiamine kinase activity
biological_process GO:0006772 - thiamine metabolic process
GO:0016310 - phosphorylation
GO:0009229 - thiamine diphosphate biosynthetic process
Note(s): Note(s): ...[more].
External database links:  
ECOCYC:
THIKIN-MONOMER
ECOLIWIKI:
b1106
INTERPRO:
IPR002575
INTERPRO:
IPR014093
INTERPRO:
IPR011009
MODBASE:
P75948
PFAM:
PF01636
PRIDE:
P75948
PRODB:
PRO_000024060
REFSEQ:
NP_415624
SMR:
P75948
UNIPROT:
P75948


Operon      
Name: hinT-ycfL-lpoB-thiK-nagZ-ycfP         
Operon arrangement:
Transcription unit        Promoter
 


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter TSS_1436 1163603 forward nd [RS-EPT-CBR] [1]
  promoter TSS_1437 1164943 forward nd [RS-EPT-CBR] [1]
  promoter TSS_1438 1165016 forward nd [RS-EPT-CBR] [1]


Evidence    

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates



Reference(s)    

 [1] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.


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