RegulonDB RegulonDB 11.2: Gene Form
   

dhaK gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

dhaR dhaK dhaL TSS_1533 TSS_1533 dhaRp dhaRp dhaKp dhaKp TSS_1532 TSS_1532 TSS_1531 TSS_1531 TSS_1530 TSS_1530 TSS_1529 TSS_1529 TSS_1528 TSS_1528 TSS_1527 TSS_1527 TSS_1526 TSS_1526 TSS_1525 TSS_1525 TSS_1524 TSS_1524 TSS_1523 TSS_1523 TSS_1522 TSS_1522

Gene      
Name: dhaK    Texpresso search in the literature
Synonym(s): ECK1188, G6627, b1200, dhaK1, ycgT
Genome position(nucleotides): 1249768 <-- 1250838
Strand: reverse
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
 53.03
Reference(s): [1] Gutknecht R., et al., 2001
[2] Paulsen IT., et al., 2000
External database links:  
ASAP:
 ABE-0004029
ECHOBASE:
 EB3660
ECOLIHUB:
 dhaK
OU-MICROARRAY:
 b1200
STRING:
 511145.b1200
COLOMBOS: dhaK


Product      
Name: dihydroxyacetone kinase subunit K
Synonym(s): DhaK, YcgT
Sequence: Get amino acid sequence Fasta Format
Cellular location: cytosol
Molecular weight: 38.215
Isoelectric point: 4.596
Motif(s):
 
Type Positions Sequence Comment
7 -> 352 DVQDVLDEQLAGLAKAHPSLTLHQDPVYVTRADAPVAGKVALLSGGGSGHEPMHCGYIGQGMLSGACPGEIFTSPTPDKIFECAMQVDGGEGVLLIIKNYTGDILNFETATELLHDSGVKVTTVVIDDDVAVKDSLYTAGRRGVANTVLIEKLVGAAAERGDSLDACAELGRKLNNQGHSIGIALGACTVPAAGKPSFTLADNEMEFGVGIHGEPGIDRRPFSSLDQTVDEMFDTLLVNGSYHRTLRFWDYQQGSWQEEQQTKQPLQSGDRVIALVNNLGATPLSELYGVYNRLTTRCQQAGLTIERNLIGAYCTSLDMTGFSITLLKVDDETLALWDAPVHTPAL UniProt: DhaK.
18 -> 351 GLAKAHPSLTLHQDPVYVTRADAPVAGKVALLSGGGSGHEPMHCGYIGQGMLSGACPGEIFTSPTPDKIFECAMQVDGGEGVLLIIKNYTGDILNFETATELLHDSGVKVTTVVIDDDVAVKDSLYTAGRRGVANTVLIEKLVGAAAERGDSLDACAELGRKLNNQGHSIGIALGACTVPAAGKPSFTLADNEMEFGVGIHGEPGIDRRPFSSLDQTVDEMFDTLLVNGSYHRTLRFWDYQQGSWQEEQQTKQPLQSGDRVIALVNNLGATPLSELYGVYNRLTTRCQQAGLTIERNLIGAYCTSLDMTGFSITLLKVDDETLALWDAPVHTPA
53 -> 56 GSGH UniProt: Dihydroxyacetone binding; Sequence Annotation Type: region of interest.
56 -> 56 H UniProt: Shows a moderate decrease in the catalytic efficiency but at least a 40- to 300-fold increase in affinity for dihydroxyacetone..
109 -> 109 D UniProt: Loss of kinase activity..

 
Classification:
Multifun Terms (GenProtEC)  
  1 - metabolism --> 1.1 - carbon utilization --> 1.1.1 - carbon compounds
Gene Ontology Terms (GO)  
cellular_component GO:0005829 - cytosol
GO:1990234 - transferase complex
molecular_function GO:0005515 - protein binding
GO:0016740 - transferase activity
GO:0016301 - kinase activity
GO:0047324 - phosphoenolpyruvate-glycerone phosphotransferase activity
GO:0004371 - glycerone kinase activity
GO:0042802 - identical protein binding
GO:0042803 - protein homodimerization activity
biological_process GO:0006071 - glycerol metabolic process
GO:0006090 - pyruvate metabolic process
GO:0006974 - cellular response to DNA damage stimulus
GO:0046365 - monosaccharide catabolic process
GO:0016310 - phosphorylation
GO:0019563 - glycerol catabolic process
GO:0046835 - carbohydrate phosphorylation
GO:0042182 - ketone catabolic process
GO:0061610 - glycerol to glycerone phosphate metabolic process
Note(s): Note(s): ...[more].
Reference(s): [3] Cheek S., et al., 2005
[4] Durnin G., et al., 2009
[5] Gonzalez R., et al., 2008
External database links:  
ALPHAFOLD:
 P76015
DIP:
 DIP-11563N
ECOCYC:
 G6627-MONOMER
ECOLIWIKI:
 b1200
INTERPRO:
 IPR012736
INTERPRO:
 IPR004006
MODBASE:
 P76015
PDB:
 3PNQ
PDB:
 4LRX
PDB:
 4LRY
PDB:
 3PNO
PDB:
 3PNM
PDB:
 3PNL
PDB:
 3PNK
PDB:
 1UOE
PDB:
 1UOD
PDB:
 1OI2
PDB:
 1OI3
PFAM:
 PF02733
PRIDE:
 P76015
PRODB:
 PRO_000022438
PROSITE:
 PS51481
REFSEQ:
 NP_415718
SMR:
 P76015
UNIPROT:
 P76015


Operon      
Name: dhaKLM         
Operon arrangement:
Transcription unit        Promoter
dhaKLM
dhaM


Transcriptional Regulation      
Display Regulation             
Activated by: DhaR


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter TSS_1522 1249128 reverse nd [RS-EPT-CBR] [6]
  promoter TSS_1523 1249497 reverse nd [RS-EPT-CBR] [6]
  promoter TSS_1524 1249736 reverse nd [RS-EPT-CBR] [6]
  promoter TSS_1525 1249868 reverse nd [RS-EPT-CBR] [6]
  promoter TSS_1526 1249893 reverse nd [RS-EPT-CBR] [6]
  promoter TSS_1527 1249898 reverse nd [RS-EPT-CBR] [6]
  promoter TSS_1528 1250032 reverse nd [RS-EPT-CBR] [6]
  promoter TSS_1529 1250044 reverse nd [RS-EPT-CBR] [6]
  promoter TSS_1530 1250148 reverse nd [RS-EPT-CBR] [6]
  promoter TSS_1531 1250415 reverse nd [RS-EPT-CBR] [6]
  promoter TSS_1532 1250830 reverse nd [RS-EPT-CBR] [6]
  promoter TSS_1533 1252373 reverse nd [RS-EPT-CBR] [6]


Evidence    

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates


Reference(s)    

 [1] Gutknecht R., Beutler R., Garcia-Alles LF., Baumann U., Erni B., 2001, The dihydroxyacetone kinase of Escherichia coli utilizes a phosphoprotein instead of ATP as phosphoryl donor., EMBO J 20(10):2480-6

 [2] Paulsen IT., Reizer J., Jin RZ., Lin EC., Saier MH., 2000, Functional genomic studies of dihydroxyacetone utilization in Escherichia coli., Microbiology 146 ( Pt 10):2343-4

 [3] Cheek S., Ginalski K., Zhang H., Grishin NV., 2005, A comprehensive update of the sequence and structure classification of kinases., BMC Struct Biol 5:6

 [4] Durnin G., Clomburg J., Yeates Z., Alvarez PJ., Zygourakis K., Campbell P., Gonzalez R., 2009, Understanding and harnessing the microaerobic metabolism of glycerol in Escherichia coli., Biotechnol Bioeng 103(1):148-61

 [5] Gonzalez R., Murarka A., Dharmadi Y., Yazdani SS., 2008, A new model for the anaerobic fermentation of glycerol in enteric bacteria: trunk and auxiliary pathways in Escherichia coli., Metab Eng 10(5):234-45

 [6] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

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