RegulonDB

Gene
Name:	puuP
Synonym(s):	ECK1291, G6643, b1296, ycjJ
Genome position(nucleotides):	1357802 <-- 1359187 Genome Browser
Strand:	reverse
Sequence:	Get nucleotide sequence FastaFormat
GC content %:	53.32
External database links:

ASAP:	ABE-0004362
ECHOBASE:	EB3666
ECOLIHUB:	puuP
OU-MICROARRAY:	b1296
STRING:	511145.b1296
COLOMBOS:	puuP

Product

Name:

putrescine:H⁺ symporter PuuP

Synonym(s):

PuuP, YcjJ

Sequence:

Get amino acid sequence Fasta Format

Cellular location:

inner membrane

Molecular weight:

50.853

Isoelectric point:

9.648

Motif(s):

Type	Positions	Sequence
	204 -> 224	LIPIITGATIVCFSFLGFDAV
	54 -> 74	HVPASYLLALAGVLFTAISYG
	110 -> 110	Y
	162 -> 182	LFVLVQISIMVVFIFLVVQGL
	242 -> 262	IFLTAVYGGVIFIAASFFMQL

Classification:

Multifun Terms (GenProtEC)
		4 - transport --> 4.2 - Electrochemical potential driven transporters --> 4.2.A - Porters (Uni-, Sym- and Antiporters)
		6 - cell structure --> 6.1 - membrane
Gene Ontology Terms (GO)
	cellular_component	GO:0016020 - membrane GO:0005886 - plasma membrane GO:0016021 - integral component of membrane
	molecular_function	GO:0015295 - solute:proton symporter activity GO:0015489 - putrescine transmembrane transporter activity
	biological_process	GO:0006974 - cellular response to DNA damage stimulus GO:0009447 - putrescine catabolic process GO:0006865 - amino acid transport GO:0055085 - transmembrane transport GO:0015847 - putrescine transport GO:1902600 - proton transmembrane transport

Note(s):

Note(s): ...[more].

External database links:

DIP:

ECOCYC:

ECOLIWIKI:

INTERPRO:

PFAM:

PRIDE:

PRODB:

REFSEQ:

UNIPROT:

Operon

Name:

puuA-ymjE-puuP

Operon arrangement:

Transcription unit	Promoter
puuP	puuPp
puuAP	puuAp

Transcriptional Regulation
	Display Regulation
Repressed by:	FNR, PuuR, ArcA

Elements in the selected gene context region unrelated to any object in RegulonDB

Type	Name	Post Left	Strand	Notes	Evidence (Confirmed, Strong, Weak)	References
promoter	TSS_1739	1357699	reverse	nd	[RS-EPT-CBR]	[1]
promoter	TSS_1740	1357701	reverse	nd	[RS-EPT-CBR]	[1]
promoter	ymjAp5	1357810	reverse	Similarity to the consensus sequence of the set of known functional promoters for this sigma factor with strong experimental evidence: good homology. Score: 4.595. P-value: 7.120e-5 Read more >	[ICWHO]	[2]

Evidence
[RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates
[ICWHO] Inferred computationally without human oversight

Reference(s)
[1] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.
[2] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78

Reference(s)

[1] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

[2] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78