RegulonDB RegulonDB 10.8: Gene Form
   

puuP gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

puuA puuP ymjA ymjE terminator puuPp puuPp ymjAp5 ymjAp5 TSS_1740 TSS_1740 TSS_1739 TSS_1739

Gene      
Name: puuP    Texpresso search in the literature
Synonym(s): ECK1291, G6643, b1296, ycjJ
Genome position(nucleotides): 1357802 <-- 1359187 Genome Browser
Strand: reverse
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
 53.32
External database links:  
ASAP:
 ABE-0004362
ECHOBASE:
 EB3666
OU-MICROARRAY:
 b1296
PortEco:
 puuP
STRING:
 511145.b1296
COLOMBOS: puuP


Product      
Name: putrescine:H+ symporter PuuP
Synonym(s): PuuP, YcjJ
Sequence: Get amino acid sequence Fasta Format
Cellular location: inner membrane
Molecular weight: 50.853
Isoelectric point: 9.648
Motif(s):
 
Type Positions Sequence
204 -> 224 LIPIITGATIVCFSFLGFDAV
54 -> 74 HVPASYLLALAGVLFTAISYG
110 -> 110 Y
162 -> 182 LFVLVQISIMVVFIFLVVQGL
242 -> 262 IFLTAVYGGVIFIAASFFMQL

 
Classification:
Multifun Terms (GenProtEC)  
  4 - transport --> 4.2 - Electrochemical potential driven transporters --> 4.2.A - Porters (Uni-, Sym- and Antiporters)
  6 - cell structure --> 6.1 - membrane
Gene Ontology Terms (GO)  
cellular_component GO:0016020 - membrane
GO:0005886 - plasma membrane
GO:0016021 - integral component of membrane
molecular_function GO:0015295 - solute:proton symporter activity
GO:0015489 - putrescine transmembrane transporter activity
GO:0022857 - transmembrane transporter activity
biological_process GO:0006974 - cellular response to DNA damage stimulus
GO:0009447 - putrescine catabolic process
GO:0006865 - amino acid transport
GO:0055085 - transmembrane transport
GO:0015847 - putrescine transport
GO:1902600 - proton transmembrane transport
Note(s): Note(s): ...[more].
External database links:  
DIP:
 DIP-11601N
ECOCYC:
 B1296-MONOMER
ECOLIWIKI:
 b1296
INTERPRO:
 IPR004841
INTERPRO:
 IPR002293
MINT:
 MINT-1252906
PANTHER:
 PTHR11785
PFAM:
 PF00324
PRIDE:
 P76037
PRODB:
 PRO_000023653
PROSITE:
 PS00218
REFSEQ:
 NP_415812
SMR:
 P76037
UNIPROT:
 P76037


Operon      
Name: puuA-ymjE-puuP         
Operon arrangement:
Transcription unit        Promoter
puuP
puuAP


Transcriptional Regulation      
Display Regulation             
Repressed by: FNR, PuuR, ArcA


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter TSS_1739 1357699 reverse nd [RS-EPT-CBR] [1]
  promoter TSS_1740 1357701 reverse nd [RS-EPT-CBR] [1]
  promoter ymjAp5 1357810 reverse Similarity to the consensus
Read more > 		[ICWHO] [2]


Evidence    

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates

 [ICWHO] Inferred computationally without human oversight


Reference(s)    

 [1] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

 [2] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78

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