RegulonDB RegulonDB 10.9: Gene Form
   

puuA gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

puuA puuD ymjE puuP ArcA PuuR CRP PuuR PuuR PuuR FNR TSS_1741 TSS_1741 puuDp puuDp puuAp puuAp puuPp puuPp

Gene      
Name: puuA    Texpresso search in the literature
Synonym(s): ECK1292, G6644, b1297, ycjK
Genome position(nucleotides): 1359490 <-- 1360908 Genome Browser
Strand: reverse
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
 53.49
External database links:  
ASAP:
 ABE-0004365
ECHOBASE:
 EB3667
ECOLIHUB:
 puuA
OU-MICROARRAY:
 b1297
STRING:
 511145.b1297
COLOMBOS: puuA


Product      
Name: glutamate-putrescine ligase
Synonym(s): PuuA, YcjK
Sequence: Get amino acid sequence Fasta Format
Cellular location: cytosol
Molecular weight: 53.177
Isoelectric point: 4.571
Motif(s):
 
Type Positions Sequence
282 -> 282 H
357 -> 357 R
135 -> 467 EPRNVLNRLWQQLRQRGLFPVVAVELEFYLLDRQRDAEGYLQPPCAPGTDDRNTQSQVYSVDNLNHFADVLNDIDELAQLQLIPADGAVAEASPGQFEINLYHTDNVLEACDDALALKRLVRLMAEKHKMHATFMAKPYEEHAGSGMHIHISMQNNRGENVLSDAEGEDSPLLKKMLAGMIDLMPSSMALLAPNVNSYRRFQPGMYVPTQASWGHNNRTVALRIPCGDRHNHRVEYRVAGADANPYLVMAAIFAGILHGLDNELPLQEEVEGNGLEQEGLPFPIRQSDALGEFIENDHLRRYLGERFCHVYHACKNDELLQFERLITETEIEW

 
Classification:
Multifun Terms (GenProtEC)  
  1 - metabolism --> 1.1 - carbon utilization --> 1.1.4 - amines
Gene Ontology Terms (GO)  
cellular_component GO:0005829 - cytosol
molecular_function GO:0003824 - catalytic activity
GO:0016874 - ligase activity
GO:0000166 - nucleotide binding
GO:0005524 - ATP binding
GO:0004356 - glutamate-ammonia ligase activity
GO:0034024 - glutamate-putrescine ligase activity
biological_process GO:0006542 - glutamine biosynthetic process
GO:0006598 - polyamine catabolic process
GO:0006807 - nitrogen compound metabolic process
GO:0009447 - putrescine catabolic process
Note(s): Note(s): ...[more].
External database links:  
ECOCYC:
 G6644-MONOMER
ECOLIWIKI:
 b1297
INTERPRO:
 IPR027303
INTERPRO:
 IPR036651
INTERPRO:
 IPR014746
INTERPRO:
 IPR008146
MODBASE:
 P78061
PFAM:
 PF00120
PRIDE:
 P78061
PRODB:
 PRO_000023649
PROSITE:
 PS00181
REFSEQ:
 NP_415813
SMART:
 SM01230
SMR:
 P78061
UNIPROT:
 P78061


Operon      
Name: puuA-ymjE-puuP         
Operon arrangement:
Transcription unit        Promoter
puuP
puuAP


Transcriptional Regulation      
Display Regulation             
Repressed by: FNR, PuuR, ArcA


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter TSS_1741 1361667 forward nd [RS-EPT-CBR] [1]


Evidence    

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates


Reference(s)    

 [1] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

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