RegulonDB RegulonDB 11.0: Gene Form
   

puuE gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

puuB puuE pspF PspF Spot 42

Gene      
Name: puuE    Texpresso search in the literature
Synonym(s): ECK1297, G6646, b1302, goaG
Genome position(nucleotides): 1365550 --> 1366815
Strand: forward
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
 57.66
External database links:  
ASAP:
 ABE-0004380
ECHOBASE:
 EB2979
ECOLIHUB:
 puuE
OU-MICROARRAY:
 b1302
STRING:
 511145.b1302
COLOMBOS: puuE


Product      
Name: 4-aminobutyrate aminotransferase PuuE
Synonym(s): GoaG, PuuE
Sequence: Get amino acid sequence Fasta Format
Cellular location: cytosol
Molecular weight: 44.729
Isoelectric point: 6.135
Motif(s):
 
Type Positions Sequence Comment
21 -> 419 MCNFFAQSAENATLKDVEGNEYIDFAAGIAVLNTGHRHPDLVAAVEQQLQQFTHTAYQIVPYESYVTLAEKINALAPVSGQAKTAFFTTGAEAVENAVKIARAHTGRPGVIAFSGGFHGRTYMTMALTGKVAPYKIGFGPFPGSVYHVPYPSDLHGISTQDSLDAIERLFKSDIEAKQVAAIIFEPVQGEGGFNVAPKELVAAIRRLCDEHGIVMIADEVQSGFARTGKLFAMDHYADKPDLMTMAKSLAGGMPLSGVVGNANIMDAPAPGGLGGTYAGNPLAVAAAHAVLNIIDKESLCERANQLGQRLKNTLIDAKESVPAIAAVRGLGSMIAVEFNDPQTGEPSAAIAQKIQQRALAQGLLLLTCGAYGNVIRFLYPLTIPDAQFDAAMKILQDAL
110 -> 111 GA UniProt: Pyridoxal phosphate binding; Sequence Annotation Type: region of interest.
238 -> 241 DEVQ UniProt: Pyridoxal phosphate binding; Sequence Annotation Type: region of interest.
267 -> 267 K UniProt: No GABA-AT activity..

 
Classification:
Multifun Terms (GenProtEC)  
  1 - metabolism --> 1.1 - carbon utilization --> 1.1.4 - amines
Gene Ontology Terms (GO)  
cellular_component GO:0005829 - cytosol
molecular_function GO:0034386 - 4-aminobutyrate:2-oxoglutarate transaminase activity
GO:0003824 - catalytic activity
GO:0016740 - transferase activity
GO:0008483 - transaminase activity
GO:0030170 - pyridoxal phosphate binding
GO:0003867 - 4-aminobutyrate transaminase activity
biological_process GO:0009447 - putrescine catabolic process
GO:0009448 - gamma-aminobutyric acid metabolic process
GO:0009450 - gamma-aminobutyric acid catabolic process
Note(s): Note(s): ...[more].
Reference(s): [1] Pham VD., et al., 2016
[2] Pham VD., et al., 2016
[3] Zhao A., et al., 2017
External database links:  
ALPHAFOLD:
 P50457
DIP:
 DIP-9825N
ECOCYC:
 G6646-MONOMER
ECOLIWIKI:
 b1302
INTERPRO:
 IPR004632
INTERPRO:
 IPR015421
INTERPRO:
 IPR015422
INTERPRO:
 IPR005814
INTERPRO:
 IPR015424
MODBASE:
 P50457
PDB:
 7JH3
PFAM:
 PF00202
PRIDE:
 P50457
PROSITE:
 PS00600
REFSEQ:
 NP_415818
SMR:
 P50457
SWISSMODEL:
 P50457
UNIPROT:
 P50457


Operon      
Name: puuDRCBE         
Operon arrangement:
Transcription unit        Promoter
puuDRCBE
puuCBE


Transcriptional Regulation      
Display Regulation             
Repressed by: CRP, PuuR, ArcA


Regulation by small RNA    
  Display Regulation
small RNA spf


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References


Reference(s)    

 [1] Pham VD., Somasundaram S., Lee SH., Park SJ., Hong SH., 2016, Engineering the intracellular metabolism of Escherichia coli to produce gamma-aminobutyric acid by co-localization of GABA shunt enzymes., Biotechnol Lett 38(2):321-7

 [2] Pham VD., Somasundaram S., Lee SH., Park SJ., Hong SH., 2016, Redirection of Metabolic Flux into Novel Gamma-Aminobutyric Acid Production Pathway by Introduction of Synthetic Scaffolds Strategy in Escherichia Coli., Appl Biochem Biotechnol 178(7):1315-24

 [3] Zhao A., Hu X., Wang X., 2017, Metabolic engineering of Escherichia coli to produce gamma-aminobutyric acid using xylose., Appl Microbiol Biotechnol 101(9):3587-3603

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