RegulonDB

Gene
Name:	puuE
Synonym(s):	ECK1297, G6646, b1302, goaG
Genome position(nucleotides):	1365550 --> 1366815 Genome Browser
Strand:	forward
Sequence:	Get nucleotide sequence FastaFormat
GC content %:	57.66
External database links:

ASAP:	ABE-0004380
ECHOBASE:	EB2979
ECOLIHUB:	puuE
OU-MICROARRAY:	b1302
STRING:	511145.b1302
COLOMBOS:	puuE

Product

Name:

4-aminobutyrate aminotransferase PuuE

Synonym(s):

GoaG, PuuE

Sequence:

Get amino acid sequence Fasta Format

Cellular location:

cytosol

Molecular weight:

44.729

Isoelectric point:

6.135

Motif(s):

Type	Positions	Sequence
	21 -> 419	MCNFFAQSAENATLKDVEGNEYIDFAAGIAVLNTGHRHPDLVAAVEQQLQQFTHTAYQIVPYESYVTLAEKINALAPVSGQAKTAFFTTGAEAVENAVKIARAHTGRPGVIAFSGGFHGRTYMTMALTGKVAPYKIGFGPFPGSVYHVPYPSDLHGISTQDSLDAIERLFKSDIEAKQVAAIIFEPVQGEGGFNVAPKELVAAIRRLCDEHGIVMIADEVQSGFARTGKLFAMDHYADKPDLMTMAKSLAGGMPLSGVVGNANIMDAPAPGGLGGTYAGNPLAVAAAHAVLNIIDKESLCERANQLGQRLKNTLIDAKESVPAIAAVRGLGSMIAVEFNDPQTGEPSAAIAQKIQQRALAQGLLLLTCGAYGNVIRFLYPLTIPDAQFDAAMKILQDAL
	110 -> 111	GA
	267 -> 267	K
	238 -> 241	DEVQ

Classification:

Multifun Terms (GenProtEC)
Multifun Terms (GenProtEC)		1 - metabolism --> 1.1 - carbon utilization --> 1.1.4 - amines
Gene Ontology Terms (GO)
	cellular_component	GO:0005829 - cytosol
	molecular_function	GO:0034386 - 4-aminobutyrate:2-oxoglutarate transaminase activity GO:0003824 - catalytic activity GO:0016740 - transferase activity GO:0008483 - transaminase activity GO:0030170 - pyridoxal phosphate binding GO:0003867 - 4-aminobutyrate transaminase activity
	biological_process	GO:0009447 - putrescine catabolic process GO:0009448 - gamma-aminobutyric acid metabolic process GO:0009450 - gamma-aminobutyric acid catabolic process

Note(s):

Note(s): ...[more].

Reference(s):

[1] Pham VD., et al., 2016
[2] Pham VD., et al., 2016
[3] Zhao A., et al., 2017

External database links:

DIP:

ECOCYC:

ECOLIWIKI:

INTERPRO:

INTERPRO:

INTERPRO:

INTERPRO:

INTERPRO:

MODBASE:

PFAM:

PRIDE:

PROSITE:

REFSEQ:

SMR:

SWISSMODEL:

UNIPROT:

Operon

Name:

puuDRCBE

Operon arrangement:

Transcription unit	Promoter
puuDRCBE	puuDp
puuCBE	puuCp

Transcriptional Regulation
	Display Regulation
Repressed by:	CRP, PuuR, ArcA

Regulation by small RNA
	Display Regulation
small RNA	spf

Elements in the selected gene context region unrelated to any object in RegulonDB

	Type	Name	Post Left	Post Right	Strand	Notes	Evidence (Confirmed, Strong, Weak)	References

Reference(s)
[1] Pham VD., Somasundaram S., Lee SH., Park SJ., Hong SH., 2016, Engineering the intracellular metabolism of Escherichia coli to produce gamma-aminobutyric acid by co-localization of GABA shunt enzymes., Biotechnol Lett 38(2):321-7
[2] Pham VD., Somasundaram S., Lee SH., Park SJ., Hong SH., 2016, Redirection of Metabolic Flux into Novel Gamma-Aminobutyric Acid Production Pathway by Introduction of Synthetic Scaffolds Strategy in Escherichia Coli., Appl Biochem Biotechnol 178(7):1315-24
[3] Zhao A., Hu X., Wang X., 2017, Metabolic engineering of Escherichia coli to produce gamma-aminobutyric acid using xylose., Appl Microbiol Biotechnol 101(9):3587-3603