RegulonDB

Gene
Name:	ycjM
Synonym(s):	ECK1304, G6647, b1309, ggaP
Genome position(nucleotides):	1370216 --> 1371895 Genome Browser
Strand:	forward
Sequence:	Get nucleotide sequence FastaFormat
GC content %:	43.87
External database links:

ASAP:	ABE-0004400
ECHOBASE:	EB3669
ECOLIHUB:	ycjM
OU-MICROARRAY:	b1309
STRING:	511145.b1309
COLOMBOS:	ycjM

Product

Name:

glucosylglycerate phosphorylase

Synonym(s):

alpha;-amylase catalytic domain family protein YcjM, GgaP, YcjM, putative glucosyltransferase

Sequence:

Get amino acid sequence Fasta Format

Cellular location:

cytosol

Molecular weight:

64.186

Isoelectric point:

6.313

Motif(s):

Type	Positions	Sequence
	84 -> 338	VHLLPFYPWSSDDGFSVIDYHQVASEAGEWQDIQQLGECSHLMFDFVCNHMSAKSEWFKNYLQQHPGFEDFFIAVDPQTDLSAVTRPRALPLLTPFQMRDHSTRHLWTTFSDDQIDLNYRSPEVLLAMVDVLLCYLAKGAEYVRLDAVGFMWKEPGTSCIHLEKTHLIIKLLRSIIDNVAPGTVIITETNVPHKDNIAYFGAGDDEAHMVYQFSLPPLVLHAVQKQNVEALCAWAQNLTLPSSNTTWFNFLASHD

Classification:

Multifun Terms (GenProtEC)
Multifun Terms (GenProtEC)		1 - metabolism
Gene Ontology Terms (GO)
	cellular_component	GO:0005829 - cytosol
	molecular_function	GO:0110068 - glucosylglycerate phosphorylase activity GO:0003824 - catalytic activity GO:0016757 - transferase activity, transferring glycosyl groups GO:0016740 - transferase activity
	biological_process	GO:0005975 - carbohydrate metabolic process

Note(s):

Note(s): ...[more].

Reference(s):

[1] Deng D., et al., 2014
[2] Deng D., et al., 2015

External database links:

CAZY:

ECOCYC:

ECOLIWIKI:

INTERPRO:

INTERPRO:

INTERPRO:

INTERPRO:

MODBASE:

PFAM:

PRIDE:

REFSEQ:

SMART:

UNIPROT:

Operon

Name:

ycjMNOPQRSTUV-ymjB-ompG

Operon arrangement:

Transcription unit	Promoter
ompG
ycjMNOPQRSTUV-ymjB-ompG

RNA cis-regulatory element

Attenuation:	Transcriptional

Elements in the selected gene context region unrelated to any object in RegulonDB

Type	Name	Post Left	Strand	Notes	Evidence (Confirmed, Strong, Weak)	References
promoter	ycjMp4	1370003	forward	Similarity to the consensus sequence of the set of known functional promoters for this sigma factor with strong experimental evidence: high homology. Score: 6.506. P-value: 1.965e-6 Read more >	[ICWHO]	[3]
promoter	ycjMp3	1370185	forward	Similarity to the consensus sequence of the set of known functional promoters for this sigma factor with strong experimental evidence: high homology. Score: 5.556. P-value: 8.632e-6 Read more >	[ICWHO]	[3]
promoter	TSS_1744	1370189	forward	nd	[RS-EPT-CBR]	[4]

Evidence
[ICWHO] Inferred computationally without human oversight
[RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates

Reference(s)
[1] Deng D., Zhang N., Mustapha A., Xu D., Wuliji T., Farley M., Yang J., Hua B., Liu F., Zheng G., 2014, Differentiating enteric Escherichia coli from environmental bacteria through the putative glucosyltransferase gene (ycjM)., Water Res 61:224-31
[2] Deng D., Zhang N., Xu D., Reed M., Liu F., Zheng G., 2015, Polymorphism of the glucosyltransferase gene (ycjM) in Escherichia coli and its use for tracking human fecal pollution in water., Sci Total Environ 537:260-7
[3] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78
[4] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.