RegulonDB RegulonDB 11.0: Gene Form
   

ycjG gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

ycjG mpaA tpx ymjC ArcA FNR TSS_1752 TSS_1752 mpaAp4 mpaAp4 mpaAp4 mpaAp4 mpaAp2 mpaAp2 TSS_1751 TSS_1751 TSS_1750 TSS_1750 ycjGp ycjGp tpxp tpxp TSS_1749 TSS_1749 TSS_1748 (cluster) TSS_1748 (cluster)

Gene      
Name: ycjG    Texpresso search in the literature
Synonym(s): ECK1321, G6661, b1325, ycjH
Genome position(nucleotides): 1388930 --> 1389895
Strand: forward
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
 54.66
Reference(s): [1] Schmidt DM., et al., 2001
External database links:  
ASAP:
 ABE-0004448
ECHOBASE:
 EB3018
ECOLIHUB:
 ycjG
OU-MICROARRAY:
 b1325
STRING:
 511145.b1325
COLOMBOS: ycjG


Product      
Name: L-Ala-D/L-Glu epimerase
Synonym(s): YcjG, YcjH
Sequence: Get amino acid sequence Fasta Format
Cellular location: cytosol
Molecular weight: 34.674
Isoelectric point: 4.698
Motif(s):
 
Type Positions Sequence Comment
1 -> 112 MRTVKVFEEAWPLHTPFVIARGSRSEARVVVVELEEEGIKGTGECTPYPRYGESDASVMAQIMSVVPQLEKGLTREELQKILPAGAARNALDCALWDLAARRQQQSLADLIG
133 -> 319 MANSASTLWQAGAKLLKVKLDNHLISERMVAIRTAVPDATLIVDANESWRAEGLAARCQLLADLGVAMLEQPLPAQDDAALENFIHPLPICADESCHTRSNLKALKGRYEMVNIKLDKTGGLTEALALATEARAQGFSLMLGCMLCTSRAISAALPLVPQVSFADLDGPTWLAVDVEPALQFTTGEL
236 -> 236 A UniProt: In Ref. 1; U33213..
244 -> 244 V UniProt: In Ref. 1; U33213..
257 -> 257 A UniProt: In Ref. 1; U33213..

 
Classification:
Multifun Terms (GenProtEC)  
  1 - metabolism --> 1.7 - central intermediary metabolism --> 1.7.1 - unassigned reversible reactions
Gene Ontology Terms (GO)  
cellular_component GO:0005829 - cytosol
molecular_function GO:0103031 - L-Ala-D/L-Glu epimerase activity
GO:0016853 - isomerase activity
GO:0046872 - metal ion binding
GO:0016855 - racemase and epimerase activity, acting on amino acids and derivatives
biological_process GO:0009063 - cellular amino acid catabolic process
GO:0009254 - peptidoglycan turnover
GO:0071555 - cell wall organization
Note(s): Note(s): ...[more].
Reference(s): [2] Vick JE., et al., 2007
External database links:  
ALPHAFOLD:
 P51981
ECOCYC:
 G6661-MONOMER
ECOLIWIKI:
 b1325
INTERPRO:
 IPR029065
INTERPRO:
 IPR018110
INTERPRO:
 IPR013342
INTERPRO:
 IPR013341
INTERPRO:
 IPR034593
INTERPRO:
 IPR036849
INTERPRO:
 IPR034603
INTERPRO:
 IPR029017
MODBASE:
 P51981
PANTHER:
 PTHR48080
PDB:
 1JPD
PFAM:
 PF13378
PFAM:
 PF02746
PRIDE:
 P51981
PROSITE:
 PS00908
PROSITE:
 PS00909
REFSEQ:
 NP_415841
SMART:
 SM00922
SMR:
 P51981
UNIPROT:
 P51981


Operon      
Name: ycjG         
Operon arrangement:
Transcription unit        Promoter
ycjG


Transcriptional Regulation      
Display Regulation             
Repressed by: PgrR


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter TSS_1748 (cluster) 1388610 reverse nd [RS-EPT-CBR] [3]
  promoter TSS_1749 1388656 reverse nd [RS-EPT-CBR] [3]
  promoter TSS_1750 1390618 reverse nd [RS-EPT-CBR] [3]
  promoter TSS_1751 1390623 reverse nd [RS-EPT-CBR] [3]
  promoter mpaAp2 1390637 reverse nd [ICWHO] [4]
  promoter mpaAp4 1390639 reverse nd [ICWHO] [4]
  promoter mpaAp4 1390639 reverse nd [ICWHO] [4]
  promoter TSS_1752 1390681 reverse nd [RS-EPT-CBR] [3]


Evidence    

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates

 [ICWHO] Inferred computationally without human oversight


Reference(s)    

 [1] Schmidt DM., Hubbard BK., Gerlt JA., 2001, Evolution of enzymatic activities in the enolase superfamily: functional assignment of unknown proteins in Bacillus subtilis and Escherichia coli as L-Ala-D/L-Glu epimerases., Biochemistry 40(51):15707-15

 [2] Vick JE., Gerlt JA., 2007, Evolutionary potential of (beta/alpha)8-barrels: stepwise evolution of a "new" reaction in the enolase superfamily., Biochemistry 46(50):14589-97

 [3] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

 [4] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78

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