RegulonDB RegulonDB 11.1: Gene Form
   

paaF gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

paaH paaE paaG paaF paaD paaI

Gene      
Name: paaF    Texpresso search in the literature
Synonym(s): ECK1390, G6714, b1393, ydbR, ydbS
Genome position(nucleotides): 1457497 --> 1458264
Strand: forward
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
 54.56
External database links:  
ASAP:
 ABE-0004659
ECHOBASE:
 EB3503
ECOLIHUB:
 paaF
MIM:
 616277
OU-MICROARRAY:
 b1393
STRING:
 511145.b1393
COLOMBOS: paaF


Product      
Name: putative 2,3-dehydroadipyl-CoA hydratase
Synonym(s): PaaF, YdbR, YdbS
Sequence: Get amino acid sequence Fasta Format
Cellular location: cytosol,inner membrane
Molecular weight: 27.237
Isoelectric point: 8.053
Motif(s):
 
Type Positions Sequence Comment
8 -> 254 RQQRVLLLTLNRPAARNALNNALLMQLVNELEAAATDTSISVCVITGNARFFAAGADLNEMAEKDLAATLNDTRPQLWARLQAFNKPLIAAVNGYALGAGCELALLCDVVVAGENARFGLPEITLGIMPGAGGTQRLIRSVGKSLASKMVLSGESITAQQAQQAGLVSDVFPSDLTLEYALQLASKMARHSPLALQAAKQALRQSQEVALQAGLAQERQLFTLLAATEDRHEGISAFLQKRTPDFKG
11 -> 11 R UniProt: In strain: W..
32 -> 32 M UniProt: In strain: W..
45 -> 45 T UniProt: In strain: W..

 
Classification:
Multifun Terms (GenProtEC)  
  1 - metabolism --> 1.1 - carbon utilization --> 1.1.1 - carbon compounds
Gene Ontology Terms (GO)  
cellular_component GO:0005829 - cytosol
GO:0005886 - plasma membrane
GO:1902494 - catalytic complex
molecular_function GO:0003824 - catalytic activity
GO:0005515 - protein binding
GO:0016829 - lyase activity
GO:0004300 - enoyl-CoA hydratase activity
GO:0042802 - identical protein binding
biological_process GO:0006629 - lipid metabolic process
GO:0006631 - fatty acid metabolic process
GO:0010124 - phenylacetate catabolic process
GO:0006635 - fatty acid beta-oxidation
Note(s): Note(s): ...[more].
Evidence: [EXP-IMP] Inferred from mutant phenotype
Reference(s): [1] Ismail W., et al., 2003
[2] Park SJ., et al., 2004
External database links:  
ALPHAFOLD:
 P76082
DIP:
 DIP-10425N
ECOCYC:
 G6714-MONOMER
ECOLIWIKI:
 b1393
INTERPRO:
 IPR018376
INTERPRO:
 IPR029045
INTERPRO:
 IPR014748
INTERPRO:
 IPR001753
MODBASE:
 P76082
PDB:
 4FZW
PFAM:
 PF00378
PRIDE:
 P76082
PROSITE:
 PS00166
REFSEQ:
 NP_415911
SMR:
 P76082
SWISSMODEL:
 P76082
UNIPROT:
 P76082


Operon      
Name: paaABCDEFGHIJK         
Operon arrangement:
Transcription unit        Promoter
paaABCDEFGHIJK
paaJ
paaJ
paaJ


Transcriptional Regulation      
Display Regulation             
Activated by: CRP, SlyA, IHF
Repressed by: PaaX


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References


Reference(s)    

 [1] Ismail W., El-Said Mohamed M., Wanner BL., Datsenko KA., Eisenreich W., Rohdich F., Bacher A., Fuchs G., 2003, Functional genomics by NMR spectroscopy. Phenylacetate catabolism in Escherichia coli., Eur J Biochem 270(14):3047-54

 [2] Park SJ., Yup Lee S., 2004, New FadB homologous enzymes and their use in enhanced biosynthesis of medium-chain-length polyhydroxyalkanoates in FadB mutant Escherichia coli., Biotechnol Bioeng 86(6):681-6

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