RegulonDB RegulonDB 11.2: Gene Form
   

glsB gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

sad dgcF glsB yneG terminator TSS_1838 TSS_1838 yneFp7 yneFp7 TSS_1837 TSS_1837

Gene      
Name: glsB    Texpresso search in the literature
Synonym(s): ECK1517, G6810, b1524, yneH
Genome position(nucleotides): 1612325 <-- 1613251
Strand: reverse
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
 52.0
External database links:  
ASAP:
 ABE-0005086
ECHOBASE:
 EB3577
ECOLIHUB:
 yneH
OU-MICROARRAY:
 b1524
STRING:
 511145.b1524
COLOMBOS: glsB


Product      
Name: glutaminase 2
Synonym(s): GlsB, YneH
Sequence: Get amino acid sequence Fasta Format
Cellular location: inner membrane
Molecular weight: 33.516
Isoelectric point: 6.402
Motif(s):
 
Type Positions Sequence Comment
24 -> 308 GKVADYIPALATVDGSRLGIAICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLVQLEMEQGIPRNPFINAGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARSEFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCVELARTFVFLANQGKAIHIDEPVVTPMQARQINALMATSGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAIAVWSPELDDAGNSLAGIAVLEQLTKQLGRSVY

 
Classification:
Multifun Terms (GenProtEC)  
  1 - metabolism
Gene Ontology Terms (GO)  
cellular_component GO:0005886 - plasma membrane
molecular_function GO:0016787 - hydrolase activity
GO:0004359 - glutaminase activity
GO:0042803 - protein homodimerization activity
biological_process GO:0006537 - glutamate biosynthetic process
GO:0006541 - glutamine metabolic process
GO:0045926 - negative regulation of growth
GO:0006543 - glutamine catabolic process
Note(s): Note(s): ...[more].
External database links:  
ALPHAFOLD:
 P0A6W0
DIP:
 DIP-12757N
ECOCYC:
 G6810-MONOMER
ECOLIWIKI:
 b1524
INTERPRO:
 IPR015868
INTERPRO:
 IPR012338
MODBASE:
 P0A6W0
PANTHER:
 PTHR12544
PFAM:
 PF04960
PRIDE:
 P0A6W0
PRODB:
 PRO_000022802
REFSEQ:
 NP_416041
SMR:
 P0A6W0
UNIPROT:
 P0A6W0


Operon      
Name: glsB-yneG         
Operon arrangement:
Transcription unit        Promoter
glsB-yneG


RNA cis-regulatory element    
Attenuation: Translational


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter TSS_1837 1611861 reverse nd [RS-EPT-CBR] [1]
  promoter yneFp7 1611874 reverse nd [COMP-AINF] [2]
  promoter TSS_1838 1611884 reverse nd [RS-EPT-CBR] [1]


Evidence    

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates

 [COMP-AINF] Inferred computationally without human oversight


Reference(s)    

 [1] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

 [2] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78

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