RegulonDB RegulonDB 11.0: Gene Form
   

sad gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

sad glsB yneJ terminator yneKp6 yneKp6 TSS_1840 TSS_1840 sadp5 sadp5 yneJp6 yneJp6 yneJp5 yneJp5

Gene      
Name: sad    Texpresso search in the literature
Synonym(s): ECK1518, G6811, b1525, yneI
Genome position(nucleotides): 1613315 <-- 1614703
Strand: reverse
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
 53.06
External database links:  
ASAP:
 ABE-0005092
ECHOBASE:
 EB3578
ECOLIHUB:
 sad
OU-MICROARRAY:
 b1525
STRING:
 511145.b1525
COLOMBOS: sad


Product      
Name: succinate semialdehyde dehydrogenase (NAD(P)+) Sad
Synonym(s): Sad, YneI
Sequence: Get amino acid sequence Fasta Format
Cellular location: cytosol
Molecular weight: 49.718
Isoelectric point: 5.347
Motif(s):
 
Type Positions Sequence Comment
10 -> 457 ISINPATGEQLSVLPWAGADDIENALQLAAAGFRDWRETNIDYRAEKLRDIGKALRARSEEMAQMITREMGKPINQARAEVAKSANLCDWYAEHGPAMLKAEPTLVENQQAVIEYRPLGTILAIMPWNFPLWQVMRGAVPIILAGNGYLLKHAPNVMGCAQLIAQVFKDAGIPQGVYGWLNADNDGVSQMIKDSRIAAVTVTGSVRAGAAIGAQAGAALKKCVLELGGSDPFIVLNDADLELAVKAAVAGRYQNTGQVCAAAKRFIIEEGIASAFTERFVAAAAALKMGDPRDEENALGPMARFDLRDELHHQVEKTLAQGARLLLGGEKMAGAGNYYPPTVLANVTPEMTAFREEMFGPVAAITIAKDAEHALELANDSEFGLSATIFTTDETQARQMAARLECGGVFINGYCASDARVAFGGVKKSGFGRELSHFGLHEFCNIQTV
136 -> 137 WN UniProt: NAD or NADP.
160 -> 163 KHAP UniProt: NAD or NADP.
212 -> 217 GSVRAG UniProt: NAD or NADP.

 
Classification:
Multifun Terms (GenProtEC)  
  1 - metabolism --> 1.1 - carbon utilization --> 1.1.1 - carbon compounds
  1 - metabolism --> 1.8 - metabolism of other compounds --> 1.8.3 - nitrogen metabolism
  5 - cell processes --> 5.6 - protection --> 5.6.2 - detoxification
Gene Ontology Terms (GO)  
cellular_component GO:0005829 - cytosol
molecular_function GO:0036243 - succinate-semialdehyde dehydrogenase (NADP+) activity
GO:0016491 - oxidoreductase activity
GO:0016620 - oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor
GO:0004777 - succinate-semialdehyde dehydrogenase (NAD+) activity
GO:0009013 - succinate-semialdehyde dehydrogenase [NAD(P)+] activity
GO:0042803 - protein homodimerization activity
GO:0004030 - aldehyde dehydrogenase [NAD(P)+] activity
biological_process GO:0006807 - nitrogen compound metabolic process
GO:0009447 - putrescine catabolic process
GO:0009450 - gamma-aminobutyric acid catabolic process
GO:0006527 - arginine catabolic process
Note(s): Note(s): ...[more].
Reference(s): [1] Chen J., et al., 2018
[2] Li ZJ., et al., 2010
[3] Song CW., et al., 2016
[4] Zhou XY., et al., 2012
External database links:  
ALPHAFOLD:
 P76149
DIP:
 DIP-12758N
ECOCYC:
 G6811-MONOMER
ECOLIWIKI:
 b1525
INTERPRO:
 IPR016163
INTERPRO:
 IPR044148
INTERPRO:
 IPR016162
INTERPRO:
 IPR016161
INTERPRO:
 IPR016160
INTERPRO:
 IPR015590
MODBASE:
 P76149
PFAM:
 PF00171
PRIDE:
 P76149
PROSITE:
 PS00070
REFSEQ:
 NP_416042
SMR:
 P76149
UNIPROT:
 P76149


Operon      
Name: sad         
Operon arrangement:
Transcription unit        Promoter
sad


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter yneJp5 1614780 forward nd [ICWHO], [RS-EPT-CBR] [5], [6]
  promoter yneJp6 1614788 forward nd [ICWHO] [5]
  promoter sadp5 1614799 reverse nd [ICWHO] [5]
  promoter TSS_1840 1614845 reverse nd [RS-EPT-CBR] [6]
  promoter yneKp6 1615550 forward nd [ICWHO] [5]


Evidence    

 [ICWHO] Inferred computationally without human oversight

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates


Reference(s)    

 [1] Chen J., Li W., Zhang ZZ., Tan TW., Li ZJ., 2018, Metabolic engineering of Escherichia coli for the synthesis of polyhydroxyalkanoates using acetate as a main carbon source., Microb Cell Fact 17(1):102

 [2] Li ZJ., Shi ZY., Jian J., Guo YY., Wu Q., Chen GQ., 2010, Production of poly(3-hydroxybutyrate-co-4-hydroxybutyrate) from unrelated carbon sources by metabolically engineered Escherichia coli., Metab Eng 12(4):352-9

 [3] Song CW., Kim JW., Cho IJ., Lee SY., 2016, Metabolic Engineering of Escherichia coli for the Production of 3-Hydroxypropionic Acid and Malonic Acid through β-Alanine Route., ACS Synth Biol 5(11):1256-1263

 [4] Zhou XY., Yuan XX., Shi ZY., Meng DC., Jiang WJ., Wu LP., Chen JC., Chen GQ., 2012, Hyperproduction of poly(4-hydroxybutyrate) from glucose by recombinant Escherichia coli., Microb Cell Fact 11:54

 [5] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78

 [6] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

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