RegulonDB RegulonDB 11.1: Gene Form
   

clcB gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

clcB mlc bidA dmsD ynfKp6 ynfKp6 TSS_1877 TSS_1877 ynfKp5 ynfKp5 TSS_1876 (cluster) TSS_1876 (cluster)

Gene      
Name: clcB    Texpresso search in the literature
Synonym(s): ECK1587, G6850, b1592, mriT, ynfJ
Genome position(nucleotides): 1665315 --> 1666571
Strand: forward
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
 54.26
External database links:  
ASAP:
 ABE-0005317
ECHOBASE:
 EB3609
ECOLIHUB:
 clcB
MIM:
 160800
MIM:
 255700
OU-MICROARRAY:
 b1592
STRING:
 511145.b1592
COLOMBOS: clcB


Product      
Name: putative chloride:H+ antiporter ClcB
Synonym(s): ClC-ec2, ClcB, MriT, YnfJ
Sequence: Get amino acid sequence Fasta Format
Cellular location: inner membrane
Molecular weight: 44.153
Isoelectric point: 8.214
Motif(s):
 
Type Positions Sequence Comment
2 -> 22 FHRLLIATVVGILAAFAVAGF UniProt: Helical.
54 -> 74 LLTPALGGLAAGLLLMGWQKF UniProt: Helical.
80 -> 402 HAPTDYMEALQTDGQFDYAASLVKSLASLLVVTSGSAIGREGAMILLAALAASCFAQRFTPRQEWKLWIACGAAAGMAAAYRAPLAGSLFIAEVLFGTMMLASLGPVIISAVVALLVSNLINHSDALLYNVQLSVTVQARDYALIISTGVLAGLCGPLLLTLMNACHRGFVSLKLAPPWQLALGGLIVGLLSLFTPAVWGNGYSTVQSFLTAPPLLMIIAGIFLCKLCAVLASSGSGAPGGVFTPTLFIGLAIGMLYGRSLGLWFPDGEEITLLLGLTGMATLLAATTHAPIMSTLMICEMTGEYQLLPGLLIACVIASVISR
146 -> 166 LWIACGAAAGMAAAYRAPLAG UniProt: Helical.
178 -> 200 MMLASLGPVIISAVVALLVSNLI UniProt: Helical.

 
Classification:
Multifun Terms (GenProtEC)  
  4 - transport
Gene Ontology Terms (GO)  
cellular_component GO:0016020 - membrane
GO:0005886 - plasma membrane
GO:0005887 - integral component of plasma membrane
GO:0016021 - integral component of membrane
GO:0034707 - chloride channel complex
molecular_function GO:0005244 - voltage-gated ion channel activity
GO:0005247 - voltage-gated chloride channel activity
GO:0005254 - chloride channel activity
biological_process GO:0006811 - ion transport
GO:0006821 - chloride transport
GO:0055085 - transmembrane transport
GO:0010447 - response to acidic pH
GO:0071468 - cellular response to acidic pH
GO:0034765 - regulation of ion transmembrane transport
GO:1902476 - chloride transmembrane transport
Note(s): Note(s): ...[more].
Reference(s): [1] Jentsch TJ., et al., 2002
External database links:  
ALPHAFOLD:
 P76175
DIP:
 DIP-28065N
ECOCYC:
 YNFJ-MONOMER
ECOLIWIKI:
 b1592
INTERPRO:
 IPR014743
INTERPRO:
 IPR001807
INTERPRO:
 IPR023790
MODBASE:
 P76175
PFAM:
 PF00654
PRIDE:
 P76175
PRINTS:
 PR00762
PRODB:
 PRO_000022295
REFSEQ:
 NP_416109
UNIPROT:
 P76175


Operon      
Name: clcB         
Operon arrangement:
Transcription unit        Promoter
clcB


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter TSS_1876 (cluster) 1667036 reverse nd [RS-EPT-CBR] [2]
  promoter ynfKp5 1667240 reverse nd [COMP-AINF] [3]
  promoter TSS_1877 1667270 reverse nd [RS-EPT-CBR] [2]
  promoter ynfKp6 1667335 reverse nd [COMP-AINF] [3]


Evidence    

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates

 [COMP-AINF] Inferred computationally without human oversight


Reference(s)    

 [1] Jentsch TJ., Stein V., Weinreich F., Zdebik AA., 2002, Molecular structure and physiological function of chloride channels., Physiol Rev 82(2):503-68

 [2] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

 [3] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78

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