RegulonDB

Gene
Name:	sufS
Synonym(s):	ECK1676, G6906, b1680, csdB, ynhB
Genome position(nucleotides):	1759303 <-- 1760523 Genome Browser
Strand:	reverse
Sequence:	Get nucleotide sequence FastaFormat
GC content %:	54.87
External database links:

ASAP:	ABE-0005610
ECHOBASE:	EB3720
ECOO157CYC:	Z2708
OU-MICROARRAY:	b1680
PortEco:	sufS
STRING:	511145.b1680
COLOMBOS:	sufS

Product

Name:

L-cysteine desulfurase

Synonym(s):

CsdB, SufS, YnhB

Sequence:

Get amino acid sequence Fasta Format

Cellular location:

cytosol

Molecular weight:

44.434

Isoelectric point:

6.326

Motif(s):

Type	Positions	Sequence
	26 -> 394	YLDSAASAQKPSQVIDAEAEFYRHGYAAVHRGIHTLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVANSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLETLPTLFDEKTRLLAITHVSNVLGTENPLAEMITLAHQHGAKVLVDGAQAVMHHPVDVQALDCDFYVFSGHKLYGPTGIGILYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEAGTPNTGGIIGLGAALEYVSALGLNNIAEYEQNLMHYALSQLESVPDLTLYGPQNRLGVIAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLMAYYNVPAMCRASLAMYNTHEEVDRL
	123 -> 123	H
	379 -> 379	R
	364 -> 364	C
	55 -> 55	H

Classification:

Multifun Terms (GenProtEC)
Multifun Terms (GenProtEC)		1 - metabolism --> 1.8 - metabolism of other compounds --> 1.8.2 - sulfur metabolism
Gene Ontology Terms (GO)
	cellular_component	GO:0005737 - cytoplasm GO:0005829 - cytosol
	molecular_function	GO:0003824 - catalytic activity GO:0005515 - protein binding GO:0016740 - transferase activity GO:0016829 - lyase activity GO:0009000 - selenocysteine lyase activity GO:0031071 - cysteine desulfurase activity GO:0030170 - pyridoxal phosphate binding GO:0042803 - protein homodimerization activity
	biological_process	GO:0006790 - sulfur compound metabolic process GO:0006534 - cysteine metabolic process GO:0016226 - iron-sulfur cluster assembly GO:0001887 - selenium compound metabolic process GO:0031162 - sulfur incorporation into metallo-sulfur cluster

Note(s):

Note(s): ...[more].

Reference(s):

[1] Bolstad HM., et al., 2010
[2] Dai Y., et al., 2015
[3] Kim S., et al., 2013
[4] Lee J., et al., 2010
[5] Mihara H., et al., 2002
[6] Prabhakar R., et al., 2005
[7] Rojas DM., et al., 2005
[8] Tokumoto U., et al., 2004

External database links:

DIP:

ECOCYC:

ECOLIWIKI:

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REFSEQ:

SMR:

UNIPROT:

Transcriptional Regulation
	Display Regulation
Activated by:	IscR, IHF, OxyR
Repressed by:	NsrR, Fur

Elements in the selected gene context region unrelated to any object in RegulonDB

Type	Name	Post Left	Strand	Notes	Evidence (Confirmed, Strong, Weak)	References
promoter	ynhGp7	1758944	reverse	Similarity to the consensus sequence of the set of known functional promoters for this sigma factor with strong experimental evidence: high homology. Score: 5.733. P-value: 6.007e-5 Read more >	[ICWHO]	[9]
promoter	ynhGp4	1758945	reverse	Similarity to the consensus sequence of the set of known functional promoters for this sigma factor with strong experimental evidence: poor homology. Score: 3.585. P-value: 4.406e-5 Read more >	[ICWHO]	[9]
promoter	sufEp3	1759481	reverse	Similarity to the consensus sequence of the set of known functional promoters for this sigma factor with strong experimental evidence: high homology. Score: 4.688. P-value: 4.027e-5 Read more >	[ICWHO]	[9]

Reference(s)
[1] Bolstad HM., Wood MJ., 2010, An in vivo method for characterization of protein interactions within sulfur trafficking systems of E. coli., J Proteome Res 9(12):6740-51
[2] Dai Y., Kim D., Dong G., Busenlehner LS., Frantom PA., Outten FW., 2015, SufE D74R Substitution Alters Active Site Loop Dynamics To Further Enhance SufE Interaction with the SufS Cysteine Desulfurase., Biochemistry 54(31):4824-33
[3] Kim S., Park S., 2013, Structural changes during cysteine desulfurase CsdA and sulfur acceptor CsdE interactions provide insight into the trans-persulfuration., J Biol Chem 288(38):27172-80
[4] Lee J., Hiibel SR., Reardon KF., Wood TK., 2010, Identification of stress-related proteins in Escherichia coli using the pollutant cis-dichloroethylene., J Appl Microbiol 108(6):2088-102
[5] Mihara H., Kato S., Lacourciere GM., Stadtman TC., Kennedy RA., Kurihara T., Tokumoto U., Takahashi Y., Esaki N., 2002, The iscS gene is essential for the biosynthesis of 2-selenouridine in tRNA and the selenocysteine-containing formate dehydrogenase H., Proc Natl Acad Sci U S A 99(10):6679-83
[6] Prabhakar R., Morokuma K., Musaev DG., 2005, A comparative study of various computational approaches in calculating the structure of pyridoxal 5'-phosphate (PLP)-dependent beta-lyase protein. The importance of protein environment., J Comput Chem 26(5):443-6
[7] Rojas DM., Vasquez CC., 2005, Sensitivity to potassium tellurite of Escherichia coli cells deficient in CSD, CsdB and IscS cysteine desulfurases., Res Microbiol 156(4):465-71
[8] Tokumoto U., Kitamura S., Fukuyama K., Takahashi Y., 2004, Interchangeability and distinct properties of bacterial Fe-S cluster assembly systems: functional replacement of the isc and suf operons in Escherichia coli with the nifSU-like operon from Helicobacter pylori., J Biochem (Tokyo) 136(2):199-209
[9] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78