RegulonDB RegulonDB 11.1: Gene Form
   

menI gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

ydiJ ydiK menI ydiH terminator TSS_1988 TSS_1988 TSS_1987 TSS_1987 ydiHp2 ydiHp2 ydiHp3 ydiHp3 TSS_1986 TSS_1986

Gene      
Name: menI    Texpresso search in the literature
Synonym(s): ECK1683, G6912, b1686, ydiI
Genome position(nucleotides): 1765222 <-- 1765632
Strand: reverse
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
 51.58
External database links:  
ASAP:
 ABE-0005628
ECHOBASE:
 EB3725
ECOLIHUB:
 ydiI
OU-MICROARRAY:
 b1686
STRING:
 511145.b1686
COLOMBOS: menI


Product      
Name: 1,4-dihydroxy-2-naphthoyl-CoA hydrolase
Synonym(s): MenI, YdiI
Sequence: Get amino acid sequence Fasta Format
Cellular location: cytosol
Molecular weight: 14.945
Isoelectric point: 7.512
Motif(s):
 
Type Positions Sequence Comment
48 -> 48 Q UniProt: 51-fold decrease in catalytic efficiency toward benzoyl-CoA..
50 -> 127 FGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSAREGRVRGVCKPLHLGSRHQVWQIEIFDEKGRLCC
54 -> 54 H UniProt: 514-fold decrease in catalytic efficiency toward benzoyl-CoA..
63 -> 63 E UniProt: Loss of activity with benzoyl-CoA as substrate..
64 -> 64 S UniProt: Almost no change in catalytic efficiency toward benzoyl-CoA..

 
Classification:
Multifun Terms (GenProtEC)  
  1 - metabolism --> 1.5 - biosynthesis of building blocks --> 1.5.3 - cofactors, small molecule carriers --> 1.5.3.11 - menaquinone, ubiquinone
Gene Ontology Terms (GO)  
cellular_component GO:0005829 - cytosol
molecular_function GO:0016787 - hydrolase activity
GO:0016790 - thiolester hydrolase activity
GO:0016289 - CoA hydrolase activity
GO:0061522 - 1,4-dihydroxy-2-naphthoyl-CoA thioesterase activity
biological_process GO:0009234 - menaquinone biosynthetic process
External database links:  
ALPHAFOLD:
 P77781
DIP:
 DIP-11750N
ECOCYC:
 G6912-MONOMER
ECOLIWIKI:
 b1686
INTERPRO:
 IPR029069
INTERPRO:
 IPR030863
INTERPRO:
 IPR003736
INTERPRO:
 IPR006683
MODBASE:
 P77781
PDB:
 4K4B
PDB:
 4K4A
PDB:
 4K49
PDB:
 1VI8
PDB:
 1SBK
PDB:
 1VH5
PFAM:
 PF03061
PRIDE:
 P77781
REFSEQ:
 NP_416201
SMR:
 P77781
UNIPROT:
 P77781


Operon      
Name: ydiJ-menI-ydiH         
Operon arrangement:
Transcription unit        Promoter
ydiJ-menI-ydiH


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter TSS_1986 1765146 reverse nd [RS-EPT-CBR] [1]
  promoter ydiHp3 1765147 reverse nd [COMP-AINF] [2]
  promoter ydiHp2 1765291 reverse nd [COMP-AINF] [2]
  promoter TSS_1987 1767630 reverse nd [RS-EPT-CBR] [1]
  promoter TSS_1988 1768977 forward nd [RS-EPT-CBR] [1]


Evidence    

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates

 [COMP-AINF] Inferred computationally without human oversight


Reference(s)    

 [1] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

 [2] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78

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