RegulonDB RegulonDB 11.1: Gene Form
   

mntP gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

rlmA mntP yobD cspC Fur MntR MntR Cis-reg; thermoregulator anti-terminator terminator terminator rlmAp9 rlmAp9 TSS_2172 TSS_2172 mntPp mntPp

Gene      
Name: mntP    Texpresso search in the literature
Synonym(s): ECK1819, G6999, b1821, yebN
Genome position(nucleotides): 1905688 --> 1906254
Strand: forward
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
 51.68
External database links:  
ASAP:
 ABE-0006065
ECHOBASE:
 EB3768
ECOLIHUB:
 yebN
OU-MICROARRAY:
 b1821
STRING:
 511145.b1821
COLOMBOS: mntP


Product      
Name: Mn2+ exporter
Synonym(s): MntP, YebN
Sequence: Get amino acid sequence Fasta Format
Cellular location: inner membrane
Molecular weight: 20.117
Isoelectric point: 7.746
Motif(s):
 
Type Positions Sequence Comment
3 -> 23 ITATVLLAFGMSMDAFAASIG UniProt: Helical.
16 -> 16 D D → A: loss of function mutation; unable to complement a ΔmntP strain in a manganese sensitivity assay and is not associated with growth cessation in an ROS sensitivity assay
31 -> 182 PKFSEALRTGLIFGAVETLTPLIGWGMGMLASRFVLEWNHWIAFVLLIFLGGRMIIEGFRGADDEDEEPRRRHGFWLLVTTAIATSLDAMAVGVGLAFLQVNIIATALAIGCATLIMSTLGMMVGRFIGSIIGKKAEILGGLVLIGIGVQIL
35 -> 35 E E → A: affects function in manganese sensitivity assay and in ROS sensitivity assay; see |CITS: [29440394]| for details
47 -> 47 E E → A: affects function in manganese sensitivity assay and in ROS sensitivity assay; see |CITS: [29440394]| for details

 
Classification:
Multifun Terms (GenProtEC)  
  4 - transport --> 4.9.B - Putative uncharacterized transport protein
  6 - cell structure --> 6.1 - membrane
Gene Ontology Terms (GO)  
cellular_component GO:0016020 - membrane
GO:0005886 - plasma membrane
GO:0005887 - integral component of plasma membrane
GO:0016021 - integral component of membrane
molecular_function GO:0005384 - manganese ion transmembrane transporter activity
biological_process GO:0006811 - ion transport
GO:0071421 - manganese ion transmembrane transport
GO:0010042 - response to manganese ion
Note(s): Note(s): ...[more].
Reference(s): [1] Kaur G., et al., 2017
[2] Kaur G., et al., 2014
[3] Price IR., et al., 2015
[4] Yamamoto K., et al., 2011
External database links:  
ALPHAFOLD:
 P76264
ECOCYC:
 G6999-MONOMER
ECOLIWIKI:
 b1821
INTERPRO:
 IPR003810
INTERPRO:
 IPR022929
PANTHER:
 PTHR35529
PFAM:
 PF02659
PRIDE:
 P76264
REFSEQ:
 NP_416335
UNIPROT:
 P76264


Operon      
Name: mntP         
Operon arrangement:
Transcription unit        Promoter
mntP


Transcriptional Regulation      
Display Regulation             
Activated by: Fur, MntR


RNA cis-regulatory element    
Attenuation: Translational


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter TSS_2172 1905462 forward nd [RS-EPT-CBR] [5]
  promoter rlmAp9 1907086 reverse nd [COMP-AINF] [6]


Evidence    

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates

 [COMP-AINF] Inferred computationally without human oversight


Reference(s)    

 [1] Kaur G., Kumar V., Arora A., Tomar A., Ashish., Sur R., Dutta D., 2017, Affected energy metabolism under manganese stress governs cellular toxicity., Sci Rep 7(1):11645

 [2] Kaur G., Sengupta S., Kumar V., Kumari A., Ghosh A., Parrack P., Dutta D., 2014, Novel MntR-independent mechanism of manganese homeostasis in Escherichia coli by the ribosome-associated protein HflX., J Bacteriol 196(14):2587-97

 [3] Price IR., Gaballa A., Ding F., Helmann JD., Ke A., 2015, Mn(2+)-sensing mechanisms of yybP-ykoY orphan riboswitches., Mol Cell 57(6):1110-1123

 [4] Yamamoto K., Ishihama A., Busby SJ., Grainger DC., 2011, The Escherichia coli K-12 MntR miniregulon includes dps, which encodes the major stationary-phase DNA-binding protein., J Bacteriol 193(6):1477-80

 [5] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

 [6] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78

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